Observation of the orientation of membrane protein crystals grown in high magnetic force fields

Numoto, Nobutaka; Shimizu, Kenichi; Matsumoto, Kazuya; Miki, Kunio; Kita, Akiko

doi:10.1016/j.jcrysgro.2013.01.003

Cited by 18 publications

(7 citation statements)

References 19 publications

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“…The impact of the magnetic field on protein crystallization was found for the first time by Japanese scientists in 1997. , The main characteristic of magnetic fields, whether homogeneous or nonhomogeneous, is that they always act differently on protein samples. Nonhomogeneous magnetic fields are responsible for the reduction of the gravity forces on the solution through the action of the magnetic force. − By applying a vertical magnetic field gradient, a magnetizing force is generated on the sample. If this force is opposite to the gravitational force, the result will be a reduction in the vertical acceleration (effective gravity) with the subsequent diminution in natural convection .…”

Section: Resultsmentioning

confidence: 99%

Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

Pareja-Rivera

Cuéllar‐Cruz

Esturau‐Escofet

et al. 2016

Crystal Growth & Design

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In this contribution we use nonconventional methods that help to increase the success rate of a protein crystal growth, and consequently of structural projects using Xray diffraction techniques. In order to achieve this purpose, this contribution presents new approaches involving more sophisticated techniques of protein crystallization, not just for growing protein crystals of different sizes by using electric fields, but also for controlling crystal size and orientation. This latter was possible through the use of magnetic fields that allow to obtain protein crystals suitable for both high-resolution X-ray and neutron diffraction crystallography where big crystals are required. This contribution discusses some pros, cons and realities of the role of electromagnetic fields in protein crystallization research, and their effect on protein crystal contacts. Additionally, we discuss the importance of room and low temperatures during data collection. Finally, we also discuss the effect of applying a rather strong magnetic field of 16.5 T, for shorts and long periods of time, on protein crystal growth, and on the 3D structure of two model proteins.

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Section: Resultsmentioning

confidence: 99%

Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

Pareja-Rivera

Cuéllar‐Cruz

Esturau‐Escofet

et al. 2016

Crystal Growth & Design

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“…They found that the orientation of multiple crystals occurred when they used different paramagnetic salts (CoCl 2 and NiCl 2 ) as precipitants at different concentrations [39]. In 2013, Numoto et al [37] reported the orientation of membrane protein (light-harvesting complex 2) crystals under high magnetic force fields for the first time; the R value and mosaicity of the crystals grown with this magnet were significantly improved compared with controls. In 2013, Cao and her co-workers [36] compared the quality of protein crystals grown in three container-less environments (agarose gel, silicone oil and diamagnetic levitation) and concluded that the diamagnetic levitation technique had the greatest benefits for obtaining high-quality protein crystals.…”

Section: Magnet Systems That Provide a Strong And Stable Magnetic mentioning

confidence: 99%

An Overview of Hardware for Protein Crystallization in a Magnetic Field

Yan

Zhang

et al. 2016

IJMS

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Protein crystallization under a magnetic field is an interesting research topic because a magnetic field may provide a special environment to acquire improved quality protein crystals. Because high-quality protein crystals are very useful in high-resolution structure determination using diffraction techniques (X-ray, neutron, and electron diffraction), research using magnetic fields in protein crystallization has attracted substantial interest; some studies have been performed in the past two decades. In this research field, the hardware is especially essential for successful studies because the environment is special and the design and utilization of the research apparatus in such an environment requires special considerations related to the magnetic field. This paper reviews the hardware for protein crystallization (including the magnet systems and the apparatus designed for use in a magnetic field) and progress in this area. Future prospects in this field will also be discussed.

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“…Lundager studied the influence of the magnetic field on the precipitations of calcium carbonate, and the results indicated that both nucleation rates and crystal growth were accelerated with the use of a magnetic field 9 . Moreover, the magnetic field can also influence the crystal size, accelerate the crystallization rate, promote crystal formation and precipitation, and enhance crystal separation process 10,11 …”

Section: Introductionmentioning

confidence: 99%

Effect of magnetic field on sodium arsenate metastable zone width and crystal nucleation kinetics for crystallization

Guan

Liu

Ling

et al. 2020

Int J of Chemical Kinetics

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Sodium arsenate, the main component of arsenic-containing solid waste pollutants, causes serious environmental health threats. Crystallization is one of the effective methods for separating and purifying sodium arsenate from arsenic-alkali residue lixivium. However, the crystallization process is limited for its low observability and the lack of separation and purification data. In this work, a laser detection system with a magnetic field generator was designed, and the solubility, metastable zone width, interfacial tension, interfacial entropy factor, crystal nucleation, and growth rate of sodium arsenate were investigated in a constant composition environment. The results showed that the solubility, metastable zone width, interfacial tension, and interfacial entropy factor decreases with the presence of a magnetic field. The magnetic field shortened the crystallization induction time and changed the nucleation and growth rate of sodium arsenate. Under the magnetic field, the nucleation rate increased from 2.43 × 10 16 to 8.98 × 10 17 (s m 3 ) −1 , and the growth rate decreased from 4.94 × 10 −8 to 2.73 × 10 −8 (s m 3 ) −1 , the growth mechanism of sodium arsenate as a continuous growth mode was unchanged. In addition, the X-ray diffraction and infrared showed that the crystal structure of sodium arsenate is unaffected by the magnetic field, indicating that the enhancement of the crystallization process of sodium arsenate with the magnetic field could be a feasible method in engineering application. K E Y W O R D S crystal nucleation, crystallization, magnetic field, metastable zone width, sodium arsenate Int J Chem Kinet. 2020;52:463-471. How to cite this article: Guan Q, Liu Y, Ling B, et al. Effect of magnetic field on sodium arsenate metastable zone width and crystal nucleation kinetics for crystallization. Int J Chem Kinet. 2020;52:463-471.

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Observation of the orientation of membrane protein crystals grown in high magnetic force fields

Cited by 18 publications

References 19 publications

Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

Recent Advances in the Understanding of the Influence of Electric and Magnetic Fields on Protein Crystal Growth

An Overview of Hardware for Protein Crystallization in a Magnetic Field

Effect of magnetic field on sodium arsenate metastable zone width and crystal nucleation kinetics for crystallization

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