A B S T R A C TThe concentration of greenhouse gases, including nitrous oxide (N 2 O), methane (CH 4 ), and compounds such as total dimethylsulfoniopropionate (DMSP t ), along with other oceanographic variables were measured in the icecovered Arctic Ocean within the Eurasian Basin (EAB). The EAB is affected by the perennial ice-pack and has seasonal microalgal blooms, which in turn may stimulate microbes involved in trace gas cycling. Data collection was carried out on board the LOMROG III cruise during the boreal summer of 2012. Water samples were collected from the surface to the bottom layer (reaching 4300 m depth) along a South-North transect (SNT), from 82.19°N, 8.75°E to 89.26°N, 58.84°W, crossing the EAB through the Nansen and Amundsen Basins. The Polar Mixed Layer and halocline waters along the SNT showed a heterogeneous distribution of N 2 O, CH 4 and DMSP t , fluctuating between 42-111 and 27-649% saturation for N 2 O and CH 4, respectively; and from 3.5 to 58.9 nmol L −1 for DMSP t . Spatial patterns revealed that while CH 4 and DMSP t peaked in the Nansen Basin, N 2 O was higher in the Amundsen Basin. In the Atlantic Intermediate Water and Arctic Deep Water N 2 O and CH 4 distributions were also heterogeneous with saturations between 52% and 106% and 28% and 340%, respectively. Remarkably, the Amundsen Basin contained less CH 4 than the Nansen Basin and while both basins were mostly under-saturated in N 2 O. We propose that part of the CH 4 and N 2 O may be microbiologically consumed via methanotrophy, denitrification, or even diazotrophy, as intermediate and deep waters move throughout EAB associated with the overturning water mass circulation. This study contributes to baseline information on gas distribution in a region that is increasingly subject to rapid environmental changes, and that has an important role on global ocean circulation and climate regulation.