Observations on Antiviral Activity of Viscosin.

Groupé, Vincent; Pugh, Leonora H.; Weiss, David S.; Kochi, Mutsuyuki

doi:10.3181/00379727-78-19071

Cited by 35 publications

(14 citation statements)

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“…For the beneficial Pseudomonas species, a range of functions and activities have been reported (Raaijmakers et al ., 2006). For example, viscosin was shown to have antiviral activity (Groupe et al ., 1951) and to cause lysis of trypomastigotes of the human pathogen Trypanosoma cruzi , the causal agent of Chagas' disease (Mercado and Colon‐Whitt, 1982). For strain P. fluorescens SBW25, we focused on the role of CLP synthesis in surface motility, biofilm formation and activity against zoospores of P. infestans , the causal agent of late blight of tomato and potato.…”

Section: Resultsmentioning

confidence: 99%

Genome‐based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species

Bruijn

Kock

Yang

et al. 2006

Molecular Microbiology

232

249

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SummaryAnalysis of microbial genome sequences have revealed numerous genes involved in antibiotic biosynthesis. In Pseudomonads, several gene clusters encoding non-ribosomal peptide synthetases (NRPSs) were predicted to be involved in the synthesis of cyclic lipopeptide (CLP) antibiotics. Most of these predictions, however, are untested and the association between genome sequence and biological function of the predicted metabolite is lacking. Here we report the genome-based identification of previously unknown CLP gene clusters in plant pathogenic Pseudomonas syringae strains B728a and DC3000 and in plant beneficial Pseudomonas fluorescens Pf0-1 and SBW25. For P. fluorescens SBW25, a model strain in studying bacterial evolution and adaptation, the structure of the CLP with a predicted 9-amino acid peptide moiety was confirmed by chemical analyses. Mutagenesis confirmed that the three identified NRPS genes are essential for CLP synthesis in strain SBW25. CLP production was shown to play a key role in motility, biofilm formation and in activity of SBW25 against zoospores of Phytophthora infestans. This is the first time that an antimicrobial metabolite is identified from strain SBW25. The results indicate that genome mining may enable the discovery of unknown gene clusters and traits that are highly relevant in the lifestyle of plant beneficial and plant pathogenic bacteria.

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Section: Resultsmentioning

confidence: 99%

Genome‐based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species

Bruijn

Kock

Yang

et al. 2006

Molecular Microbiology

232

249

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“…As more Ps-CLPs were isolated, specific families or “groups” were defined with chemically similar Ps-CLPs and named for a particular prototype Ps-CLP as shown in Figure 1 . These include the viscosin ( Groupé et al, 1951 ), orfamide ( Gross et al, 2007 ; Ma et al, 2016a ), amphisin ( Sorensen et al, 2001 ), syringomycin ( Segre et al, 1989 ), syringopeptin ( Ballio et al, 1991 ), and tolaasin groups ( Rainey et al, 1991 ; Bassarello et al, 2004 ). New CLPs are continuously found in numerous environments, and can be assigned to an existing group ( D’Aes et al, 2014 ; Weisshoff et al, 2014 ; Johnston et al, 2015 ; Michelsen et al, 2015a ; Zachow et al, 2015 ; Ma et al, 2016a ; Gotze et al, 2017 ) or constitute a new group, such as the recently discovered bananamides, ( Nguyen et al, 2016 ) and xantholysins ( Li et al, 2013 ).…”

Section: Pseudomonas Spp Produce a Large Variety Of Clpsmentioning

confidence: 99%

“…As a result, data on antiviral activity of Ps-CLPs appears quite sparse. Nevertheless, the very first report on the lipodepsipeptide viscosin in 1951 already mentioned the inhibition and inactivation of human pathogenic viruses such as the infectious bronchitis virus, influenza A virus, and Newcastle disease virus ( Groupé et al, 1951 ). Recently, Shimura et al (2013) described the antiviral activity of the xantholysin-like lipopeptide MA026 against Hepatitis C.…”

Section: Pseudomonas Spp Produce a Large Variety Of Clpsmentioning

confidence: 99%

Cyclic Lipodepsipeptides From Pseudomonas spp. – Biological Swiss-Army Knives

2018

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Cyclic lipodepsipeptides produced by Pseudomonas spp. (Ps-CLPs) are biosurfactants that constitute a diverse class of versatile bioactive natural compounds with promising application potential. While chemically diverse, they obey a common structural blue-print, allowing the definition of 14 distinct groups with multiple structurally homologous members. In addition to antibacterial and antifungal properties the reported activity profile of Ps-CLPs includes their effect on bacterial motility, biofilm formation, induced defense responses in plants, their insecticidal activity and anti-proliferation effects on human cancer cell-lines. To further validate their status of potential bioactive substances, we assessed the results of 775 biological tests on 51 Ps-CLPs available from literature. From this, a fragmented view emerges. Taken as a group, Ps-CLPs present a broad activity profile. However, reports on individual Ps-CLPs are often much more limited in the scope of organisms that are challenged or activities that are explored. As a result, our analysis shows that the available data is currently too sparse to allow biological function to be correlated to a particular group of Ps-CLPs. Consequently, certain generalizations that appear in literature with respect to the biological activities of Ps-CLPs should be nuanced. This notwithstanding, the data for the two most extensively studied Ps-CLPs does indicate they can display activities against various biological targets. As the discovery of novel Ps-CLPs accelerates, current challenges to complete and maintain a useful overview of biological activity are discussed.

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“…The second and third hits were both associated to the BGC involved in the production of erythrochelin, a non-ribossomally assembled siderophore isolated from the actinobacterium, Saccharopolyspora erythraea [86]. Additional matches included pyoverdine [87], also a siderophore and viscosin, a biosurfactant [88] and antibiotic [89] NRP, which were both isolated from Pseudomonas strains.…”

Section: Assigned Bgc/metabolites To Ad Domainmentioning

confidence: 99%

Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica

et al. 2020

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Bacterial natural products (NPs) are still a major source of new drug leads. Polyketides (PKs) and non-ribosomal peptides (NRP) are two pharmaceutically important families of NPs and recent studies have revealed Antarctica to harbor endemic polyketide synthase (PKS) and non-ribosomal peptide synthetase (NRPS) genes, likely to be involved in the production of novel metabolites. Despite this, the diversity of secondary metabolites genes in Antarctica is still poorly explored. In this study, a computational bioprospection approach was employed to study the diversity and identity of PKS and NRPS genes to one of the most biodiverse areas in maritime Antarctica-Maxwell Bay. Amplicon sequencing of soil samples targeting ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively, revealed abundant and unexplored chemical diversity in this peninsula. About 20% of AD domain sequences were only distantly related to characterized biosynthetic genes. Several PKS and NRPS genes were found to be closely associated to recently described metabolites including those from uncultured and candidate phyla. The combination of new approaches in computational biology and new culture-dependent and -independent strategies is thus critical for the recovery of the potential novel chemistry encoded in Antarctica microorganisms. target the highly conserved ketosynthase (KS) and adenylation (AD) domains of PKS and NRPS genes, respectively. A growing number of studies have employed the PCR-based sequence tag approach to uncover PKS and NRPS gene diversity across different environmental microbiomes [7][8][9].Extreme environments, such as Antarctica, have the potential to reveal novel molecules since they are still a poorly explored source of chemical scaffolds and novel chemical diversity [10,11]. As such, metabarcoding approaches to study PKS and NRPS diversity in Antarctic ecosystems represent a simple and effective strategy to understand the biosynthetic potential of a large number of sampling sites [7]. A couple of such studies have been carried out very recently in Antarctica [12,13] revealing that soils at different Antarctica locations harbor multiple endemic PKS and NRPS genes, likely to be involved in the synthesis of new chemical diversity. In Antarctic soils, the percentage of yet-uncultured genera is expected to be much higher [14] than the 82% described for general soils [15]. A considerable fraction of such uncultured bacteria corresponds to members of the NP-rich Actinobacteria [16,17] and Cyanobacteria [18,19] phyla.King George Island is one of the largest ice-free and biodiverse areas in maritime Antarctica [20]. It harbors a high density of scientific stations, particularly in Maxwell Bay [21], where most of terrestrial biology research takes place. Although bacterial diversity studies have been conducted in this area [22,23], the diversity of bacterial biosynthetic genes has not been a target of study. Here, we perform a detailed study on soil samples from Maxwell Bay, with the main goal of assessing the...

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Observations on Antiviral Activity of Viscosin.

Cited by 35 publications

References 0 publications

Genome‐based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species

Genome‐based discovery, structure prediction and functional analysis of cyclic lipopeptide antibiotics in Pseudomonas species

Cyclic Lipodepsipeptides From Pseudomonas spp. – Biological Swiss-Army Knives

Diversity of Bacterial Biosynthetic Genes in Maritime Antarctica

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