Observations on development and morphological effects of histamine liberator 48/80 on PAS‐positive granular leukocytes and heterophils of <i>Catostomus commersoni</i>

Barber, D. L.; Westermann, J. E. Mills

doi:10.1111/j.1095-8649.1978.tb03469.x

Cited by 22 publications

(10 citation statements)

References 15 publications

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“…This process is essentially identical to that reported for EGC of the trout intestine with release of granules being preceded by appearance of perigranular halos and a reticulated appearance of the granule contents [25,26]. The homogeneous appearance of these granules was similar to that reported for most fish EGC and some mammalian MC [14,15,26,27]. Complex ''crystalline'' or ''scroll'' sub-structures often reported in mammalian MC granules and occasionally in fish EGC were not observed in the damselfish.…”

Section: Discussionsupporting

confidence: 63%

“…Exposure of EGC in the submucosa of the rainbow trout (Oncorhynchus mykiss) and the white sucker (Catostomus commersoni) to agents such as compound 48/80 was reported to result in significant decreases in the number of EGC observable with light microscopy, apparently due to a loss of sufficient granules to allow identification of these cells by light microscopy (as confirmed using electron microscopy) with a secondary loss due to emigration of EGC to other tissue regions [14,26,27]. In contrast, degranulating cells in the damselfish were readily observed and scored as such by light microscopy in both the gut and in tumors.…”

Section: Discussionmentioning

confidence: 93%

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Degranulation of eosinophilic granule cells in neurofibromas and gastrointestinal tract in the bicolor damselfish

Schmale

Vicha

Cacal

2004

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 93%

Degranulation of eosinophilic granule cells in neurofibromas and gastrointestinal tract in the bicolor damselfish

Schmale

Vicha

Cacal

2004

Fish & Shellfish Immunology

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“…However, both PAS-GLs and basophils were present in the peripheral blood of N. ater in this study, and the concurrent presence of metachromatic basophils and PAS-GLs has been reported in other teleost species, such as S. lima (Bianchi et al 2014). This evidence suggests that PAS-GLs may not be the evolutionary precursor of basophils, as suggested by Barber and Westermann (1978b) ater were similar to those described in other Siluriformes such as I. punctatus (Williams and Warner 1976;Tavares-Dias and de Moraes 2007) and moustache catfish (Synodontis membranacea) (Owolabi 2011). Metachromatic staining of muccopoysaccharide granules in toluidine blue occurs in mammalian basophils (Cooper and Cruickshank 1966), and the use of 0.5% alkaline (pH 9) toluidine blue, as used in this study, has revealed metchromatic granules within basophils in several fish species (Tavares-Dias 2006b).…”

Section: Discussionmentioning

confidence: 63%

“…Since many of these histamine-induced morphological changes were similar to those observed in mammalian basophils and mast cells during histamine release, and basophils were absent in the peripheral blood of C. commersoni, it was suggested that PAS-GLs may be functionally equivalent to, or evolutionary precursors of, basophils or mast cells (Barber and Westermann 1978b;Tavares-Dias 2006a). However, both PAS-GLs and basophils were present in the peripheral blood of N. ater in this study, and the concurrent presence of metachromatic basophils and PAS-GLs has been reported in other teleost species, such as S. lima (Bianchi et al 2014).…”

Section: Discussionmentioning

confidence: 91%

“…Similar cells with a weak PAS reaction, as seen in N. graeffei and N. midgleyi in this study, have been reported by Zinkl et al (1991) in granular leukocytes of goldfish (Carassius auratus). In white suckers (Catostomus commersoni), injection of compound 48/80, which promotes histamine release, altered the morphology and number of PAS-GLs (Barber and Westermann 1978b).…”

Section: Discussionmentioning

confidence: 99%

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The haematology of six species of native catfish from northern Australia

Kelly

Gibson-Kueh

2015

Aust. J. Zool.

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Haematology has the potential to be a valuable tool in determining the health status of wild fish populations and wider ecosystem health. However, limited haematological data are available for wild . graeffei, N. midgleyi, N. ater and T. tandanus. This is the first report on the leukocyte morphology and cytochemistry of any native Australian catfish species, and provides useful baseline data for future assessments of fish health and ecosystem integrity in Australia.

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Mast cells enter a teleost's brain by Xth cranial nerve in response toDiplostomum phoxini (Trematoda)

Ali

1984

Experientia

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Summary. Experimental infection by Diplostomum phoxini of the brain of laboratory hatched and reared Phoxinus phoxinus induces the migration along the Xth cranial nerve of periodic acid Schiff positive granular leucocytes (PAS-GLs).These differentiate and grow into cells that lie between the parasite and the neurones of the host. The transformed ceils are associated with elevated levels of heparin and serotonin in the brain; little histamine was detected. The reactive cell is identified as a mast cell.More than 1000 metacercariae of the trematode Diplostomum phoxini can live in the brain of the teleost Phoxinus phoxinus (the European minnow) and although the parasite is associated with a host response 2 it survives for at least 2 years 3. The host can be induced to encapsulate and kill D.phoxini in the brain 4 but it has not been recorded to do so in natural conditions. The cells of the capsule, but not those of the normal response, were identified as astrocytes 4 but in view of the reported abundance of ependymal cells in the teleost brain 5 the participation of ependymal cells in capsule formation cannot be ruled out. The origin and nature of the cells that contribute to the normal response have not been determined although following an appreciation of the extreme solubility of the cell's contents, some 2 histochemical data was recorded. The course of the normal host cell reponse has now been studied through the experimental infection with D.phoxini of young, laboratory hatched and raised minnows.Materials' and methods. Spermatozoa stripped from 1 male P.phoxinus were used to fertilize ova from a single female minnow collected from Lake Frongoch. This lake has been the source of material used in a number of studies of D.phoxini 6-9. Artificially fertilized eggs were maintained in the laboratory at 18 ~ in running lake water and they hatched in 7 days. The young fish were kept at 18 ~ and they measured 1.4-2.3 cm and weighed 60-123 mg at 89 days of age. The gonads in the young fish at this time were developing and primary oocytes were observed in females; the fish were judged to be physiologically normal. These fish were then infected with about 50 cercariae of D.phoxini from 1 specimen of Lymnaea peregrer collected in Lake Frongoch. The infected fish were kept at 18 ~ and individuals were killed 4, 6, 8, h; 3, 4, 6, 8, 10, 12, 15, 20, 25, 29, 30, 34 days post-infection. The fish were fixed in Rossman's fixative 2, embedded without decalcification in Paraplast (Lancer, Oxford), most serial sections were stained with PAS, the use of aqueous solutions was avoided 2. A few sections were stained in PAS using the standard technique J~ to determine the water solubility of intracellular granules. Because the fish were small it was possible to transversely section whole animals. Furthermore, because they had been kept isolated from other fish in the aquarium they were free of pathogens except

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Observations on development and morphological effects of histamine liberator 48/80 on PAS‐positive granular leukocytes and heterophils of Catostomus commersoni

Cited by 22 publications

References 15 publications

Degranulation of eosinophilic granule cells in neurofibromas and gastrointestinal tract in the bicolor damselfish

Degranulation of eosinophilic granule cells in neurofibromas and gastrointestinal tract in the bicolor damselfish

The haematology of six species of native catfish from northern Australia

Mast cells enter a teleost's brain by Xth cranial nerve in response toDiplostomum phoxini (Trematoda)

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