Observations on<i>Aeromonas hydrophila</i>septicaemia in a patient with leukaemia

Abrams, Elizabeth A.; Zierdt, Charles H.; Brown, Joe R.

doi:10.1136/jcp.24.6.491

Cited by 18 publications

(7 citation statements)

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“…A. punctata is also associated with joint infections, meningitis, peritonitis, and infection of the eyes, while their involvement in gastroenteritis remains controversial to some degree [24]. The infection rate of A. punctata is higher in individuals who have either chronic diseases [7,16] or a compromised immune system [2,17]. A. punctata harbors antibiotic resistance plasmid pFBAOT6 which enables it to become immune against all major classes of antimicrobial drugs [39].…”

Section: Discussionmentioning

confidence: 99%

“…Aeromonas is considered to be an opportunistic or secondary pathogen. Most reports of Aeromonas infections describe patients who were either immunologically compromised [2,17] or having chronic diseases [7,16]. A. punctata harbors antibiotic resistance plasmid pFBAOT6 which enables it to become immune against all major classes of antimicrobial drugs [39].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Partial Characterization of a Virulent Bacteriophage IHQ1 Specific for Aeromonas punctata from Stream Water

et al. 2011

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Aeromonas punctata is the causative agent of septicemia, diarrhea, wound infections, meningitis, peritonitis, and infections of the joints, bones and eyes. Bacteriophages are often considered alternative agents for controlling bacterial infection and contamination. In this study, we described the isolation and preliminary characterization of bacteriophage IHQ1 (family Myoviridae) active against the Gram-negative bacterial strain A. punctata. This virulent bacteriophage was isolated from stream water sample. Genome analysis indicated that phage IHQ1 was a double-stranded DNA virus with an approximate genome size of 25-28 kb. The initial characterization of this newly isolated phage showed that it has a narrow host range and infects only A. punctata as it failed to infect seven other clinically isolated pathogenic strains, i.e., methicillin-resistant Staphylococcus aureus 6403, MRSA 17644, Acinetobacter 33408, Acinetobacter 1172, Pseudomonas aeruginosa 22250, P. aeruginosa 11219, and Escherichia coli. Proteomic pattern of phage IHQ1, generated by SDS-PAGE using purified phage particles, showed three major and three minor protein bands with molecular weights ranging from 25 to 70 kDa. The adsorption rate of phage IHQ1 to the host bacterium was also determined, which was significantly enhanced by the addition of 10 mM CaCl(2). From the single-step growth experiment, it was inferred that the latent time period of phage IHQ1 was 24 min and a burst size of 626 phages per cell. Moreover, the pH and thermal stability of phage IHQ1 were also investigated. The maximum stability of the phage was observed at optimal pH 7.0, and it was totally unstable at extreme acidic pH 3; however, it was comparatively stable at alkaline pH 11.0. At 37°C the phage showed maximum number of plaques, and the viability was almost 100%. The existence of Aeromonas bacteriophage is very promising for the eradication of this opportunistic pathogen and also for future applications such as the design of new detection and phage typing (diagnosis) methods. The specificity of the bacteriophage for A. punctata makes it an attractive candidate for phage therapy of A. punctata infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolation and Partial Characterization of a Virulent Bacteriophage IHQ1 Specific for Aeromonas punctata from Stream Water

et al. 2011

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“…The resistance of Aeromonas to penicillin and ampicillin is probably due to the production of beta-lactamase (Bulger and Sherris, 1966). Aeromonas species are consistently sensitive to chloramphenicol and tetracycline and Abrams, Zierdt, and Brown (1971) have suggested that these antibiotics be given in combination as soon as a tentative diagnosis of Aeromonas infection is made. However, in patients with Aeromonas septicaemia the use of an appropriate bactericidal antibiotic such as kanamycin should also be considered.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Aeromonas species from clinical sources

McCracken¹,

Barkley²

1972

J Clin Pathol

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SYNOPSIS In a period of one year, in a general hospital, Aeromonas hydrophila was isolated from 13 patients and Aeromonas shigelloides from one patient. Eight of the patients had superficial infections, two had urinary tract infections, and four had bacteriaemia. The association of Aeromonas bacteriaemia with cirrhosis of the liver and malignant disease, which has been previously reported, was observed in three of the four bacteriaemic patients. The key to laboratory diagnosis of this genus is the routine performance of the oxidase test in bacteriological procedures for the identification of Gram-negative bacilli.Organisms belonging to the genus Aeromonas are seldom associated with human infections. Nevertheless, there are indications from sources in the United States and Australia that, in common with 'Present address:

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“…The resistance of the patient influences the type of organism. Johanson, Pierce, and Sanford (1969) report that Gramnegative colonization of the pharynx is common in the sick but rare in the healthy hospital patient even when no antibiotics are used, while Gramnegative bacillaemia is extensively reported in immunosuppressed patients whether the immunosuppression is due to steroids, uraemia or cytotoxic drugs (Brumfitt and Leigh, 1969;Grant, Horowitz, and Lorian, 1969;Abrams, Zierdt, and Brown, 1971 ;Mummery et al, 1971).…”

Section: Discussionmentioning

confidence: 99%

Bacteraemia after open-heart surgery

Lockey¹,

Gonzalez‐Lavin²,

Ray³

et al. 1973

Thorax

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We have analysed the bacteraemias occurring before discharge from hospital in patients subjected to open-heart surgery. The overall incidence was 2-7%. The predominant organisms at first were staphylococci and streptococci, but in the later years of the study almost all positive blood cultures were due to Gram-negative bacilli. Short-term prophylactic antibiotics were used throughout. Eleven of the 12 Gram-positive bacteraemias occurred after the period of antibiotic prophylaxis; eight of the patients had endocarditis and a further three had serious wound infections. Most of the 32 Gram-negative bacillaemias occurred while antibiotics were being given. Some were transient phenomena in well patients; others were associated with terminal states. Infection was a major cause of death in four patients, however, and two patients had endocarditis. Serratia, providence, hafnia, and citrobacter were all identified as well as the more usual escherichia, klebsiella, and pseudomonas. We discuss the possible origins and management of these bacteraemias.

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Observations onAeromonas hydrophilasepticaemia in a patient with leukaemia

Cited by 18 publications

References 1 publication

Isolation and Partial Characterization of a Virulent Bacteriophage IHQ1 Specific for Aeromonas punctata from Stream Water

Isolation and Partial Characterization of a Virulent Bacteriophage IHQ1 Specific for Aeromonas punctata from Stream Water

Isolation of Aeromonas species from clinical sources

Bacteraemia after open-heart surgery

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