Observations on the Effects of Insulin Introduced Into the Medium of Tissue Cultures

Gey, George O.; Thalhimer, William

doi:10.1001/jama.1924.26520460003014d

Cited by 82 publications

(18 citation statements)

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“…Al ready a few years after the detection of insu lin. it was shown that insulin enhances in vitro growth of chick fibroblasts [6]. This growth-promoting effect of insulin has been shown since then for many culture systems of different cells.…”

Section: Discussionmentioning

confidence: 82%

Trophic Effect of Portal Blood in Maintenance Cultures of Adult Rat Hepatocytes

Junge¹,

Creutzfeldt²

1981

Digestion

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The hepatotrophic effect of portal serum was demonstrated in primary monolayer cultures of adult rat hepatocytes: DNA synthesis in cultures incubated for 4 days with pooled portal serum of fed rats was significantly higher than in cultures with peripheral serum from the same animals. This difference was not observed when portal blood was treated with charcoal or obtained from fasting rats. Portal serum from diabetic rats stimulated DNA synthesis less than portal control serum. The trophic effect of portal serum from normal rats was enhanced after tolbutamide injection. The lost trophic effect of charcoal-treated portal serum was completely restored when insulin was added to reobtain a normal portal immunoreactive insulin concentration. Thus, insulin seems to be the main factor responsible for the hepatotrophic action of portal blood in vitro.

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Section: Discussionmentioning

confidence: 82%

Trophic Effect of Portal Blood in Maintenance Cultures of Adult Rat Hepatocytes

Junge¹,

Creutzfeldt²

1981

Digestion

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“…Furthermore, the basal medium must contain an essential, so called, ITS supplement (insulin, transferrin and selenium). Insulin, the first of the components of the ITS supplement, has been known to be essential in cell culture from 1924 and is now the most commonly used hormone in culture media (Gey and Thalhimer, 1924). Transferrin is also an essential protein in culture medium where the main action is to transfer iron into the cells (Bjare, 1992).…”

Section: Basal Mediummentioning

confidence: 99%

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Valk

Brunner

Smet³

et al. 2010

Toxicology in Vitro

500

379

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“…Insulin is required for cellular metabolism of glucose and is thus necessary for cell culture. 25 Transferrin is an essential protein that is known to transport iron into cells, where iron plays a role in the hydroxylation of proline during collagen synthesis, 26,27 and selenium is an essential trace element that boosts the activity of glutathione peroxidases in shielding cells from oxidative stress. 28 ITS-supplemented media have been shown to viably support and promote matrix deposition within chondrocyte-seeded agarose constructs.…”

Section: Introductionmentioning

confidence: 99%

Insulin, Ascorbate, and Glucose Have a Much Greater Influence Than Transferrin and Selenous Acid on theIn VitroGrowth of Engineered Cartilage in Chondrogenic Media

Cigáň

Nims

Albro

et al. 2013

Tissue Engineering Part A

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The primary goal of this study was to characterize the response of chondrocyte-seeded agarose constructs to varying concentrations of several key nutrients in a chondrogenic medium, within the overall context of optimizing the key nutrients and the placement of nutrient channels for successful growth of cartilage tissue constructs large enough to be clinically relevant in the treatment of osteoarthritis (OA). To this end, chondrocyte-agarose constructs (ø4 · 2.34 mm, 30 · 10 6 cells/mL) were subjected to varying supplementation levels of insulin (0· to 30· relative to standard supplementation), transferrin (0· to 30·), selenous acid (0· to 10·), ascorbate (0· to 30· ), and glucose (0· to 3·). The quality of resulting engineered tissue constructs was evaluated by their compressive modulus (E -Y ), tensile modulus (E +Y ), hydraulic permeability (k), and content of sulfated glycosaminoglycans (sGAG) and collagen (COL); DNA content was also quantified. Three control groups from two separate castings of constructs (1· concentrations of all medium constituents) were used. After 42 days of culture, values in each of these controls were, respectively, E -Y = 518 -78, 401 -113, 236 -67 kPa; E +Y = 1420 -430, 1140 -490, 1240 -280 kPa; k = 2.3 -0.8 · 10 -3 , 5.4 -7.0 · 10 -3 , 3.3 -1.3 · 10 -3 mm 4 /N$s; sGAG = 7.8 -0.3, 6.3 -0.4, 4.1 -0.5%/ww; COL = 1.3 -0.2, 1.1 -0.3, 1.4 -0.4%/ww; and DNA = 11.5 -2.2, 12.1 -0.6, 5.2 -2.8 mg/disk. The presence of insulin and ascorbate was essential, but their concentrations may drop as low as 0.3· without detrimental effects on any of the measured properties; excessive supplementation of ascorbate (up to 30·) was detrimental to E -Y , and 30· insulin was detrimental to both E +Y and E -Y . The presence of glucose was similarly essential, and matrix elaboration was significantly dependent on its concentration ( p < 10 -6

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Observations on the Effects of Insulin Introduced Into the Medium of Tissue Cultures

Cited by 82 publications

References 0 publications

Trophic Effect of Portal Blood in Maintenance Cultures of Adult Rat Hepatocytes

Trophic Effect of Portal Blood in Maintenance Cultures of Adult Rat Hepatocytes

Optimization of chemically defined cell culture media – Replacing fetal bovine serum in mammalian in vitro methods

Insulin, Ascorbate, and Glucose Have a Much Greater Influence Than Transferrin and Selenous Acid on theIn VitroGrowth of Engineered Cartilage in Chondrogenic Media

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