Observations regarding DNA replication sites in human cells in vivo following infusions of iododeoxyuridine and bromodeoxyuridine

Abstract

In studies using bromodeoxyuridine (BrdUrd) and/or iododeoxyuridine (IdUrd) to label S phase cells in cancer patients, several unique observations were made regarding DNA replication sites and the organization of newly synthesized DNA in post-mitotic cells. While the majority of tumour specimens removed at the end of infusions demonstrated concentration of replication sites around the nuclear membrane, biopsies obtained in leukaemic patients 1 week later demonstrated several distinct patterns of labelling. For example, one, two or all lobes of granulocytes were labelled. Scavenger macrophages bearing labelled leukaemic cells in their cytoplasm were also seen. Sequential IdUrd/BrdUrd labelling of solid tumours showed various patterns of nuclear/nucleolar/membrane labelling, allowing more precise localization of early versus late replication sites.

A high‐resolution, double‐labeling method for the study of in vivo red blood cell aging

“…Here, we describe a simple double‐labeling technique that allows for identification of a narrow, age‐specific cell cohort throughout the RBC life span. Analogous to studies of the mitotic cell cycle, 11,12 two distinct labeling steps are separated by a well‐defined time interval. Cells are subsequently classified by the combination of labels they possess.…”

Culture-Independent Identification of Microorganisms That Respond to Specified Stimuli

“…However, the growth kinetics for specific bacterial cultures obtained with and without BrdU were shown to be similar (20). In addition, if used at modest concentrations, BrdU appears to be relatively safe and is currently being administered to human cancer patients (3,15). Despite these potential shortcomings, both of these reports demonstrate an approach that can assist in the investigation of uncultured microorganisms.…”

Kinetic Analysis of Tumor Cells in Bone and Soft Tissue Sarcomas.

“…In addition to our study with nude mice, studies with combined use of double-staining and flow cytometry [18,23] and a study on observation of DNA replication site [21] have been reported, indicating the usefulness of this double-staining. Although no reports are available in the field of orthopaedics to date, this study preserving the tissue architecture will become more important in orthopaedics, because histopathological pleomorphism and heterogeneity are the characteristics of bone and soft tissues tumors.…”