Observing the development of the temporomandibular joint in embryonic and post-natal mice using various staining methods

Liang, Wenna; Li, Xihai; Gao, Bizhen; Gan, Huijuan; Lin, Xuejuan; Liao, Linghong; Li, Candong

doi:10.3892/etm.2015.2937

Cited by 26 publications

(22 citation statements)

References 51 publications

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“…Mice are representative models for the study of TMJ development and physiopathology . In contrast to rats, mice exhibit lateral mandibular movements similar to humans .…”

Section: Discussionmentioning

confidence: 99%

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Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction

Balanta‐Melo

Torres‐Quintana

Bemmann

et al. 2018

J of Oral Rehabilitation

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Summary Background Masseter muscle function influences mandibular bone homeostasis. As previously reported, bone resorption markers increased in the mouse mandibular condyle two days after masseter paralysis induced with botulinum toxin type A (BoNTA), followed by local bone loss. Objective This study aimed to evaluate the bone quality of both the mandibular condyle and alveolar process in the mandible of adult mice during the early stage of a BoNTA‐induced masseter muscle atrophy, using a combined 3D histomorphometrics and shape analysis approach. Methods Adult BALB/c mice were divided into an untreated control group and an experimental group; the latter received one single BoNTA injection in the right masseter (BoNTA‐right) and saline in the left masseter (Saline‐left). 3D bone microstructural changes in the mandibular condyle and alveolar process were determined with high‐resolution microtomography. Additionally, landmark‐based geometric morphometrics was implemented to assess external shape changes. Results After 2 weeks, masseter mass was significantly reduced (P‐value <0.001). When compared to Saline‐left and untreated condyles, BoNTA‐right condyles showed significant bone loss (P‐value <0.001) and shape changes. No significant bone loss was observed in the alveolar processes of any of the groups (P‐value >0.05). Conclusion Condyle bone quality deteriorates at an early stage of BoNTA‐induced masseter muscle atrophy, and before the alveolar process is affected. Since the observed bone microstructural changes resemble those in human temporomandibular joint degenerative disorders, the clinical safety of BoNTA intervention in the masticatory apparatus remains to be clarified.

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“…Mice are representative models for the study of TMJ development and physiopathology . In contrast to rats, mice exhibit lateral mandibular movements similar to humans .…”

Section: Discussionmentioning

confidence: 99%

“…Mice are representative models for the study of TMJ development and physiopathology. 34,35 In contrast to rats, mice exhibit lateral mandibular movements similar to humans. 36 This feature of masticatory kinematics allows the use of mice for understanding basic processes behind masticatory movement disorders.…”

Section: Discussionmentioning

confidence: 99%

Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction

Balanta‐Melo

Torres‐Quintana

Bemmann

et al. 2018

J of Oral Rehabilitation

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“…It plays an important role in mediating chondrocyte-chondrocyte and chondrocyte-matrix interactions through its ability to bind hyaluronan. 32 We did not observe any variation in aggrecan expression in fibrocartilage chondrocytes between K/BxN and control mice.…”

Section: Discussionmentioning

confidence: 48%

Temporomandibular joint damage in K/BxN arthritic mice

et al. 2020

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Rheumatoid arthritis (RA) is an autoimmune disease affecting 1% of the world population and is characterized by chronic inflammation of the joints sometimes accompanied by extra-articular manifestations. K/BxN mice, originally described in 1996 as a model of polyarthritis, exhibit knee joint alterations. The aim of this study was to describe temporomandibular joint (TMJ) inflammation and damage in these mice. We used relevant imaging modalities, such as micro-magnetic resonance imaging (μMRI) and micro-computed tomography (μCT), as well as histology and immunofluorescence techniques to detect TMJ alterations in this mouse model. Histology and immunofluorescence for Col-I, Col-II, and aggrecan showed cartilage damage in the TMJ of K/BxN animals, which was also evidenced by μCT but was less pronounced than that seen in the knee joints. μMRI observations suggested an increased volume of the upper articular cavity, an indicator of an inflammatory process. Fibroblast-like synoviocytes (FLSs) isolated from the TMJ of K/BxN mice secreted inflammatory cytokines (IL-6 and IL-1β) and expressed degradative mediators such as matrix metalloproteinases (MMPs). K/BxN mice represent an attractive model for describing and investigating spontaneous damage to the TMJ, a painful disorder in humans with an etiology that is still poorly understood.

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“…This alignment is important for optimum tensile strength of various tissues. 15,17,18 Moreover, the development of condylar articular cartilage displays a special multilevel organization, which consists of a thin, shallow zone of flat and tightly bound cells, the polymorphic (pm)/ancestral (pr) layer, where chondroprogenitors actively multiply and produce chondrocytes with suitable growth. The lower zones contain flattened/mature and hypertrophic chondrocytes, where cells undergo the endochondral bone formation.…”

Section: Discussionmentioning

confidence: 99%

<p>Single Nucleotide Polymorphisms (SNPs) of COL1A1 and COL11A1 in Class II Skeletal Malocclusion of Ethnic Javanese Patient</p>

Ardani¹,

Aulanni’am²,

Diyatri³

2020

CCIDE

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Background: The prevalence of malocclusion cases in the orthodontic specialist clinic in Airlangga University's Dental Hospital in Surabaya, Indonesia, in 2014-2016 is fairly high, as 55.34% of the occurrences were identified as class II skeletal malocclusion. This type of skeletal malocclusion, which is usually recognized in adults, occurs as a result of variation during growth and development. Lately, there have been many reports on gene polymorphisms of COL1A1 and COL11A1, which are assumed to be associated with class II skeletal malocclusion in Caucasians. Purpose: This study aims to analyze the relationship between single nucleotide polymorphisms (SNPs) of COL1A1 and COL11A1 with class II skeletal malocclusion in Javanese ethnic group patients with mandibular micrognathism. Materials and Methods: The diagnosis of class II skeletal malocclusion was established using the lateral cephalometric radiographs (ANB angle ≥4°) (n=50). DNA was extracted from the patient's peripheral blood. After that, PCR, electrophoresis, and DNA sequencing were conducted on the extracted DNA based on COL1A1 and COL11A1 primers. Results: The SNPs in COL1A1 are c.20980G/A in 27 patients and c.20980G>A in 8 patients, whereas SNPs in COL11A1 are both c.134373C/A and c.134555C/T in 8 patients and both c.[134373A>C] and c.134582G>A in 10 patients. All samples show the deletion (c. [134227delA]) in COL11A1. Conclusion: SNPs in COL1A1 and COL11A1 have been found in class II skeletal malocclusion of Javanese ethnic group patients. Seventy percent of SNPs in COL1A1 occur in rs.2249492, whereas 36% of newly discovered SNPs appear in COL11A1. All samples also have deletion in COL11A1.

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Observing the development of the temporomandibular joint in embryonic and post-natal mice using various staining methods

Cited by 26 publications

References 51 publications

Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction

Masseter muscle atrophy impairs bone quality of the mandibular condyle but not the alveolar process early after induction

Temporomandibular joint damage in K/BxN arthritic mice

<p>Single Nucleotide Polymorphisms (SNPs) of COL1A1 and COL11A1 in Class II Skeletal Malocclusion of Ethnic Javanese Patient</p>

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