Occupational Hazards of Xylene and its Preventive Measures

Sumathy, Govindarajan; Sathyapriya, Bhaskaran; Chandrakala, B; Koshy, Jinu Merlin; Govindaraj, Jayamathi; Padmavathy, Kesavaram

doi:10.37506/v10/i12/2019/ijphrd/192342

Cited by 3 publications

(2 citation statements)

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“…[ 6 ] One such agent is cedarwood oil which is a safe, natural alternative, eco-friendly, nonflammable, readily available, easy to handle and specifically it is noncarcinogenic. [ 7 ] Cedarwood oil is an essential oil derived from various types of conifers, most in the pine or cypress botanical families. It is produced from the foliage, and sometimes the wood, roots and stumps.…”

Section: Introductionmentioning

confidence: 99%

Cedarwood oil as an alternative to xylene as a clearing agent in histopathological tissue processing – A comparative study

Thamilselvan

Sherlin

Jayaraj

et al. 2021

Journal of Oral and Maxillofacial Pathology

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Background: Clearing in histopathological tissue processing should be able to make the tissues translucent and clear for the tissues to be visible under light microscopy and should render the clearing agent to be miscible with the dehydrant and the impregnation wax in the preceding and following processing steps. Xylene is a gold standard clearing agent but increasing concerns about the potential carcinogenicity, implementing eco-friendly agents in routine histopathology is necessary. Aim: The aim of the study is to assess the clearing ability of Cedarwood oil as an alternative to Xylene in routine tissue processing. Materials & Methods: The study was carried out in the Department of Oral Pathology and Microbiology. Formalin fixed 50 tissue samples of size 3-7mm were taken and subsequent dehydration done with acetone and alcohol. The dehydrated tissue is later processed using 90ml of Cedarwood oil with few drops of Xylene and Thymol. After clearing the tissues were subjected to impregnation and embedded in paraffin wax, later which sections were made and stained using H & E stain. Results: The results of our study on comparison showed better outcome in tissues processed with cedarwood oil than xylene. Statistical Significant correlation was observed in nuclear staining (p value = 0.001) ; cytoplasmic staining (p value = 0.08) and background staining (p value = 0.045) indicating a positive results on using cedarwood oil as clearing agent. Conclusion: The cedarwood oil can be considered as a safer natural alternative to xylene in laboratories. The cedarwood oil is eco - friendly and easily available with enhanced tissue processing qualities.

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Section: Introductionmentioning

confidence: 99%

Cedarwood oil as an alternative to xylene as a clearing agent in histopathological tissue processing – A comparative study

Thamilselvan

Sherlin

Jayaraj

et al. 2021

Journal of Oral and Maxillofacial Pathology

View full text Add to dashboard Cite

show abstract

“…Also disposal of this volatile compound is always a problem. On comparison, Cedarwood oil is a safe, natural alternative, eco-friendly, nonflammable, effortlessly available, and easy to deal with and especially its non-carcinogenic [5]. The major advantage of all is that cedarwood oil does not cause any damage to the tissues processed.…”

Section: Introductionmentioning

confidence: 99%

Cedarwood Oil as a Substitute for Xylene in Histopathological Laboratories – A Systematic Review

Viswasini

Ramani

Ramasubramanian

2021

JPRI

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Background: Xylene is an aromatic hydrocarbon that's extensively used as a deparaffinizing agent, and it's far extraordinarily biohazardous. It is proven to be the best chemical to be used in histopathological laboratories in spite of its carcinogenic potential. Aim: The aim of this study is to do a systematic review on the efficacy of cedarwood oil as clearing agent and deparaffinizing agent alternative to xylene in histopathological laboratories. Materials and Methods: The articles for this review were searched from PubMed, Google Scholar, Google search and manual search using the keywords cedarwood oil, natural substitute, natural alternative and xylene. The final of 3 articles were included in the review which compared the efficacy of cedarwood oil as a natural alternative with the gold standard xylene. Once the articles to be reviewed were finalized, data was collected, tabulated and was verified and interpreted. Results: Three articles included in this review showed considerable and acceptable results with cedarwood oil as clearing and deparaffinizing agent. Conclusion: Merits and demerits are equally found in both the reagents but compromising the time and cost in terms of cedarwood oil for health benefits hold good for the pathologists but bad for the patients. In case of xylene, compromising the health benefits of workers which results in quicker diagnosis hold good for the patients but not for the pathologists. Finally storage and disposal of these two reagents still remains questionable.

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An Upgradation to Minimize Hazardous Effects in Histopathological Laboratories: Cedarwood Oil as an Alternative to Xylene

Chaduvula,

Jadeja,

Anerao

et al. 2024

JBSMFS

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Background: In traditional histopathology, xylene is used as a clearing agent following haematoxylin and eosin (H&E) staining of tissue sections. However, xylene presents significant health risks, prompting the search for safer alternatives. Essential oils, including cedarwood oil, have been proposed as potential replacements. This study evaluates the efficacy of cedarwood oil as a clearing agent compared to xylene. Aim: To assess the clearing effectiveness of cedarwood oil as an alternative to xylene in routine haematoxylin and eosin staining. Materials and Methods: Twenty paraffin blocks from routine biopsy specimens were used. Cedarwood oil, sourced from a local organic supplier, was tested alongside xylene. Paraffin sections (4 microns) from each block were cleared using either 8% cedarwood oil or xylene and subsequently stained with H&E. The quality of staining was evaluated based on nuclear and cytoplasmic detail, clarity, and uniformity. Results: Cedarwood oil demonstrated a significant correlation with xylene concerning all assessed staining parameters. Conclusion: Cedarwood oil is a viable, eco-friendly, and safer alternative to xylene for use as a clearing agent in histopathological laboratories.

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Occupational Hazards of Xylene and its Preventive Measures

Cited by 3 publications

References 0 publications

Cedarwood oil as an alternative to xylene as a clearing agent in histopathological tissue processing – A comparative study

Cedarwood oil as an alternative to xylene as a clearing agent in histopathological tissue processing – A comparative study

Cedarwood Oil as a Substitute for Xylene in Histopathological Laboratories – A Systematic Review

An Upgradation to Minimize Hazardous Effects in Histopathological Laboratories: Cedarwood Oil as an Alternative to Xylene

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