Background:
Clearing in histopathological tissue processing should be able to make the tissues translucent and clear for the tissues to be visible under light microscopy and should render the clearing agent to be miscible with the dehydrant and the impregnation wax in the preceding and following processing steps. Xylene is a gold standard clearing agent but increasing concerns about the potential carcinogenicity, implementing eco-friendly agents in routine histopathology is necessary.
Aim:
The aim of the study is to assess the clearing ability of Cedarwood oil as an alternative to Xylene in routine tissue processing.
Materials & Methods:
The study was carried out in the Department of Oral Pathology and Microbiology. Formalin fixed 50 tissue samples of size 3-7mm were taken and subsequent dehydration done with acetone and alcohol. The dehydrated tissue is later processed using 90ml of Cedarwood oil with few drops of Xylene and Thymol. After clearing the tissues were subjected to impregnation and embedded in paraffin wax, later which sections were made and stained using H & E stain.
Results:
The results of our study on comparison showed better outcome in tissues processed with cedarwood oil than xylene. Statistical Significant correlation was observed in nuclear staining (p value = 0.001) ; cytoplasmic staining (p value = 0.08) and background staining (p value = 0.045) indicating a positive results on using cedarwood oil as clearing agent.
Conclusion:
The cedarwood oil can be considered as a safer natural alternative to xylene in laboratories. The cedarwood oil is eco - friendly and easily available with enhanced tissue processing qualities.
Oral malignancies are a group of dangerous disease affecting the oral cavity. The subtypes of oral malignancies have distinctive aetiology, epidemiology and multi-year endurance rate. This retrospective study included subjects who reported to the Department of Oral Pathology and Microbiology in Saveetha Dental College from July 2019 - March 2020. Data on age, gender, lesion site, habit profile and diagnosis were recorded and analysed for Pearson’s chi-square correlation test between demographic data and oral malignancies. A total of 83 cases were included in the study in which 80 (96.3%) are carcinomas, and 3 (3.75%) are other malignancies. The correlation between the oral malignancy diagnosis and demographic data - age, gender, site and habit showed statistically insignificant (p>0.05) results with p-value = 0.802, 0.898, 0.07and 0.817 respectively. Our study demonstrated that predominance of oral malignancies was seen in the fifth - the seventh decade of life with more propensity towards the male population, and buccal mucosa was identified as the most common site. This study reflects the need to create awareness among the general population regarding the burden of disease and the possibility of preventing it.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.