This research shows the size composition of airborne dust fractions in selected dairy barns down to the smallest particles, including factors that influence this composition. Measurements with a Dust-Track 8530 laser photometer took place in the summer at external temperatures of 29.5 to 36 °C. In barns with straw bedding, the average total dust concentration TDC was 66.98 ± 28.38 μg·m−3 (PM10 60.11 ± 19.93 μg·m−3, PM4 49.48 ± 13.76 μg·m−3, PM2.5 44.78 ± 10.18 μg·m−3, and PM1 38.43 ± 9.29 μg·m−3). In barns without straw bedding, the average TDC was 55.91 ± 36.6 μg·m−3, PM10 33.71 ± 13.86 μg·m−3, PM4 30.69 ± 15.29 μg·m−3, PM2.5 27.02 ± 13.38 μg·m−3, and PM1 22.93 ± 10.48 μg·m−3. The largest TDC of 108.09 ± 32.93 μg·m−3 (PM10 69.80 ± 18.70 μg·m−3, PM4 68.20 ± 18.41 μg·m−3, PM2.5 53.27 ± 14.73 μg·m−3, and PM1 38.46 ± 5.55 μg·m−3) was measured in an old cowshed with stanchion housing for 113 cows, straw bedding, and ventilation through windows. In a modern cowshed for loose housing of 440 lactating cows without straw bedding, with natural ventilation and 24 axial fans, TDC was 53.62 ± 49.52 μg·m−3, PM10 20.91 ± 5.24 μg·m−3, PM4 17.11 ± 3.23 μg·m−3, PM2.5 13.71 ± 0.92 μg·m−3, and PM1 12.69 ± 2.82 μg·m−3. In all investigated barns, a large proportion of airborne dust particles (54.38 ± 20.82% of TDC) consists of the smallest PM1 dust particles (from 12.69 ± 2.82 μg·m−3 to 48.48 ± 1.18 μg·m−3).