Excess nitrate (NO 3 ) in the MississippiRiver is the leading cause of hypoxia in the Gulf of Mexico (Rabalais et al. 1996; Turner et al. 2006; USEPA-SAB 2007), and excess NO 3 has been identified as a leading contributor to hypoxia zones at over 300 locations in US estuaries and coastal waters (CENR 2010). In the Mississippi River basin, much of the NO 3 comes from row crop production where large amounts of nitrogen (N) fertilizer are used to optimize production of annual crops that essentially grow only four months of the year. The upper Midwest in particular is the dominant source of NO 3 to the Mississippi River (Robertson et al. 2009), and it has been shown that most of the NO 3 enters surface waters through an extensive network of subsurface drain pipes (Jaynes et al. 1999; Goolsby et al. 2001; Royer et al. 2006; David et al. 2010). These drainage pipes, often Dan B. Jaynes is a soil scientist with the National Laboratory for Agriculture and the Environment, Abstract: Drainage water management (DWM) is a potentially valuable management practice for reducing NO 3 losses to surface waters in areas of artificial drainage. But the practice is essentially untested in Midwest United States conditions and its water quality and crop yield benefits uncertain. This paper reports results from applying DWM to a 22 ha (54 ac) production field in central Iowa as part of a five-state Conservation Innovative Grant effort to document the impact of DWM across the Midwest. Three of nine plots in an existing tile drainage research site were retrofitted with control structures so that the drainage level could be controlled. Water flow from the tile in each plot, NO 3 concentration in the drainage, and crop yield were measured over a four year period from 2006 to 2009. The field was in a two year corn (Zea mays L.)-soybean (Glycine max [L.] Merr.) rotation with nitrogen (N) fertilizer applied before the corn crop only. During four years of monitoring tile flow, there was a significant (p = 0.05) 21% decrease in tile flow, no significant decrease in NO 3 concentration, and a significant 29% reduction in NO 3 load leaching from the DWM treatment compared to conventional drainage. No yield benefits from DWM were observed for the two year average for corn (2006 and 2008), but a significant yield increase of 8% was observed for the two year average for soybean (2007 and 2009). For the four years monitored in this study, it is unclear if the yield increase for soybean versus no increase for corn was due to weather patterns or because corn and soybean responded differently to the raised water table caused by DWM.