Occurence and Antibiogram of Salmonella Spp. In Raw and Fermented Milk in Zaria and Environs

Tamba, Zainab; Bello, M.; Raji, M. A.

doi:10.3329/bjvm.v14i1.28850

Cited by 7 publications

(9 citation statements)

References 11 publications

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“…isolated from milk samples were 100% sensitive to gentamicin, neomycin and ciprofloxacin, and 100% resistant to erythromycin, doxycycline and amoxicilin. Other authors also found Salmonella isolates of raw and fermented milk origin as 100% sensitive to ciprofloxacin and gentamicin and resistant to erythromycin (85.7%) and amoxicillin (78.6%) (Tamba et al, 2016;Munsi et al, 2015).…”

Section: Discussionmentioning

confidence: 90%

Detection of Multi–drug Resistant Salmonella From Milk and Meat in Bangladesh

Rahman

Rahman²,

Islam³

et al. 2018

Bangl. J. Vet. Med.

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This study was conducted to investigate the prevalence of Salmonella spp. in milk, chicken meat and beef and to determine the multi-drug resistance (MDR) profile of Salmonella spp. in Mymensingh and Gazipur districts, Bangladesh. A total of 169 samples of milk (n=108), chicken meat (n=51) and beef (n=10) were collected from Bangladesh Agricultural University (BAU) dairy farm, American dairy farm, Gazipur and different small dairy farms of municipal area during July 2016 to June 2017. Salmonella spp. were isolated on various selective agar media such as: Salmonella-Shigella (SS) agar, Xylose-Lysine Deoxycholate (XLD) agar, Eosine-Methylene Blue (EMB) agar. Identification of Salmonella spp. was done by colony characteristics, Gram staining, biochemical test and Polymerase Chain Reaction (PCR). Multi-drug resistant Salmonella spp. was detected by disc diffusion test using 10 commonly used antibiotics. The overall prevalence of Salmonella spp. in all food samples was 21.89%. A total of 29 (56.86%) chicken meat, 02 (1.85%) milk, and 06 (60%) beef samples were Salmonella spp. positive. Antibiogram study showed that an overall 89.19% of Salmonella spp. was found multi-drug resistant. Specifically 100%, 66.67% and 93.10% of the Salmonella spp. isolates originated from milk, beef and chicken meat respectively were multi-drug resistant. The result of this study suggests that MDR Salmonella spp. is prevalent in the milk and meat which might cause public health hazard if proper hygienic measures are not undertaken at farm and marketing level.

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Section: Discussionmentioning

confidence: 90%

Detection of Multi–drug Resistant Salmonella From Milk and Meat in Bangladesh

Rahman

Rahman²,

Islam³

et al. 2018

Bangl. J. Vet. Med.

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“…Creek road Mile one Rumuokoro revealed a 14(100%) susceptibility to Salmonella spp. to the antibiotic as reported by Tamba et al, [28] in Zaria, Nigeria. The report of Islam et al [27] is not in agreement with the findings in this study, Islam et al [27] reported 16(100%) susceptibility of Salmonella spp.…”

Section: Spp Count Of the Various Sample Parts From The Three Marketsmentioning

confidence: 55%

“…recorded corroborates report by Selvaraj et al, [33] The underlying mechanism of β-lactam antibiotic resistance is through the production of βlactamases. These enzymes function by hydrolysing the β-lactam ring by breaking the amide linkage, thereby disabling their capability of inhibiting bacterial cell wall synthesis [28]. Previously, it was known that ESBL-producing bacteria thrived commonly in hospital and clinical settings where extensive use of antimicrobial drugs and agents assisted in the development of their antimicrobial resistance [33].…”

Section: Spp Count Of the Various Sample Parts From The Three Marketsmentioning

confidence: 99%

Antibiogram of Listeria and Salmonella Species Isolated from Tilapia Fish (Oreochromis nitolicus) and Snail (Archachatina marginata) Sold in Port Harcourt, Nigeria

Odu

Ogbonna

Daminabo

2021

JAMB

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Land snails are ubiquitous creatures found on farms, forests and domesticated in homes. Bacteriological studies indicate that various potentially pathogenic bacteria inhabit different organs and tissues including the haemolymph of the African giant snail, the presence of pathogenic bacteria in the snails are a health threat to consumers and may cause food borne diseases/ infection. Pollution of aquatic environments with organic waste of animal and human origin may lead to transfer of pathogens to the fishes, thereby making them carriers of the pathogens. In this study antibiogram studies were carried out on the isolates from snail and tilapia fish to determine antibiogram of Listeria monocytogenes and Salmonella species isolated from tilapia fishes and snails sold in Port Harcourt. One hundred and thirty two (132) samples were obtained from three different markets namely; Creek road, Mile one and Rumuokoro markets over a period of six months. Standard analytical protocols were employed to determine the bacteriological characteristics of the various parts such as intestine, flesh/meat, gills and fluid(snail). Antibiogram of Listeria and Salmonella species were determined using standard methods as recommended by CLSI. Statistical analyses were carried out using ANOVA and All pairs tukey-kramer. Results of Total heterotrophic bacterial count shows that snail samples had the highest number of bacterial count compared to frozen tilapia fishes. Mean Listeria spp. count for frozen tilapia fish ranged from 2.7 ±0.68 x104cfu/g to 2.9 ±0.23 x104cfu/g (flesh), 3.3 ±0.15 x104 to 3.7 ±0.35 x104cfu/g (gill), 3.8 ±0.44 x104 to 4.3 ±0.57 x104 cfu/g (Intestine), across the three markets, Mean Listeria spp. count for snail sample ranged from 0.7 ±0.29 x104 to 1.1 ±0.18 x104cfu/ml (Snail fluid) 1.1 ±0.18 x104 to 1.2 ±0.16 x104cfu/g (meat), 1.6 ±0.44 x104 to 1.9 ±0.57 x104cfu/g (Intestine) Creek road market. Mean total Salmonella count for frozen tilapia fish ranged from 1.0 ±0 x103cfu/g to 1.3 ±0.58 x103 (flesh), 1.0 ± 0 x103 to 1.6 ± 0.58 x103cfu/g (gills), 1.2 ±0.5 x103 cfu/g to 2 .0 ±1.41 x103 cfu/g (Intestine), across the three markets. Mean total Salmonella count for snail ranged from 1.0 ±0.58 x103 to 1.3 ±0 x103cfu/ml (fluid), 1.5 ±1.0 x103 to 1.7 ±0.96 x103cfu/g (meat), 1.7 ±0.96 x103cfu/g to 3.0 ±1.58 x103cfu/g (Intestine), across the three markets. Results of antibiogram revealed that all the Listeria species were 100% susceptible to Levofloxacin, Ofloxacin, Gentamycin, Azithromycin, Erythromycin and Ceftriaxone-sulbactam but 100% resistance to Augmentin, Ciprofloxacin and Cefuxime while Salmonella species were 100% susceptible to Ofloxacin and Ciprofloxacin and resistance to Cetazidime and Gentamicin, thus, these drugs should be considered the drug of choice for infections caused by these bacteria,

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“…This result is in sharp disagreement with previous studies that revealed a 14(100%) susceptibility to Salmonella spp. to the antibiotic reported by Tamba et al [21] in Zaria, Nigeria. Islam et al [20] also reported an alteration to the findings in this study with their 16(100%) susceptibility of Salmonella pp.…”

Section: Resultsmentioning

confidence: 84%

Prevalence and Antibiogram of Salmonella Species Isolated from Snail (Archachatina marginata) Sold in Port Harcourt, Rivers State, Nigeria

Daminabo

Ogbonna

Odu

et al. 2020

EJNFS

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Increase in microbial population especially Salmonella species in food due to improper handling, storage and exposure to contaminants can raise public health concerns when consumed without adequate processing. This study evaluates the prevalence and antibiogram of Salmonella species associated with the giant land Snail (Archachatina marginata) sold in markets around Port Harcourt metropolis. A total number of seventy two (72) samples of land snail were collected from three markets; Creek Road, Mile one and Rumuokoro. The samples were labelled and transported in an ice packed coolers to the laboratory for analyses. Standard microbiological protocols were employed to determine the microbial load and species of the various parts (intestine, meat and fluid) of the snail samples after shucking. Antibiotics sensitivity profile testing of the isolated and identified Salmonella species were carried out as recommended by Clinical Laboratory Standard Institute (CLSI) and statistical analyses using one way ANOVA and all pairs Turkey-Kramer. Results from the study showed that the highest total heterotrophic bacteria count (THBC) of 8.6x106CFU/g was obtained in the snail intestine sourced from Creek road market while THBCs of 8.2x106 CFU/g and 7.3x106 CFU/g were from Mile one and Rumuokoro markets respectively. THBCs of meat from the markets ranged from 4.3-5.4x106 CFU/g and 3.7-4.9x106CFU/ml in fluid with Rumuokoro having the least occurrence respectively. Mean Salmonella counts (MSCs) ranged from 0.4-3.6 x103 CFU/g, with least count obtained from Rumuokoro and Mile 1 markets. Similarly, least MSCs in fluid and intestine were obtained from Rumuokoro and Mile 1 whereas Creek road Market had the highest respectively. Three species of Salmonella; S. arizonae, S. gallinarum and S. typhi were predominant in addition to other species such as Vibrio spp,, Bacillus spp., Staphylococcus spp., Shigella spp., Pseudomonas spp., Enterobacter spp., E. coli, Micrococcus spp., Acinetobacter spp., Klebsiella spp. and Listeria spp identified using both conventional and molecular method. Antibiogram profile revealed that all the identified Salmonella species were susceptible to Ofloxacin and Ciprofloxacin but strongly resistance to Cetazidime and Gentamicin. The diversity and elevated microbial load observed from this study calls for caution in handling and processing of snails since most of these bacteria may become aetiologic agents of several food-borne diseases and other pathological conditions. As a necessity, good quality control measures and proper chemotherapy should be administered to patients with signs and symptoms of food borne illness emanating from consumption of snail.

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Occurence and Antibiogram of Salmonella Spp. In Raw and Fermented Milk in Zaria and Environs

Cited by 7 publications

References 11 publications

Detection of Multi–drug Resistant Salmonella From Milk and Meat in Bangladesh

Detection of Multi–drug Resistant Salmonella From Milk and Meat in Bangladesh

Antibiogram of Listeria and Salmonella Species Isolated from Tilapia Fish (Oreochromis nitolicus) and Snail (Archachatina marginata) Sold in Port Harcourt, Nigeria

Prevalence and Antibiogram of Salmonella Species Isolated from Snail (Archachatina marginata) Sold in Port Harcourt, Rivers State, Nigeria

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