Occurrence and Antibiotic Resistance of Conform Bacteria and Antimicrobial Residues in Pasteurized Cow’s Milk from Brazil

Abstract

In this study we examined the hygienic and sanitary quality of pasteurized cow's milk in the state of Paraná, Brazil, by determining the presence of coliforms and occurrence of antimicrobial residues. A total of 260 milk samples were collected from commercial establishments in different regions of the state. Coliform populations were estimated by the multiple-tube test, and antimicrobial residues were detected by enzyme-linked immunosorbent assay. Overall, 105 samples (40.4%) were unsuitable for consumption according to Brazilian legal standards. Among the coliforms, Escherichia coli and Klebsiella pneumoniae were respectively identified in 77.05 and 36.07% of the samples. The highest rates of resistance to antimicrobial agents were observed for ampicillin (19.2%), cephalothin (18.9%), and tetracycline (17.1%). Antimicrobial residues were detected in 80 samples (30.8%). Forty-eight samples (18.5%) were positive for tetracycline, 29 (17.4%) for neomycin, 9 (3.5%) for beta-lactams, 6 (2.3%) for gentamicin, 4 (1.5%) for chloramphenicol, and 1 (0.4%) for streptomycin-dihydrostreptomycin. The results demonstrate a high prevalence of coliforms and also a high occurrence of antimicrobial residues in pasteurized cow's milk from Paraná, Brazil.

Survey on Antimicrobial Residues in Raw Milk and Antimicrobial Use in Dairy Farms in the Emilia-Romagna Region, Italy

“…However, aminoglycosides were not investigated in the Croatian survey. The antimicrobial residues in pasteurized milk from Brazil observed by Zanella et al (2010) included aminoglycosides, tetracycline and β lactams, and were similar to our findings, except for the high antimicrobial residues (30.8%) which were, however, below the MRLs permitted in Brazil.…”

Genetic heterogeneity of Escherichia coli isolated from pasteurized milk in State of Paraná, Brazil

“…The isolates were stored in Tryptic Soy Broth (TSB; Difco, Le Pont de Claix, France) with glycerol at -20 ºC and kept at the Laboratory of Food Microbiology, Department of Clinical Analysis and Biomedicine, Maringá State University. The milk samples were obtained from 21 dairies in Paraná, Brazil, from March 2006 to November 2008 in a previous study conducted in our laboratory that evaluated the presence of coliforms in pasteurized milk (Zanella et al, 2010). The different dairies were identified by the abbreviations AA, APRO, CAIUÁ, CC, CIA, COC, COOP, GM, L, LAC, LOU, MOU, MP, NE, P, PN, S, SN, SO, TB, and U.…”

Isolation, characterization and antibiotics susceptibility of -glucuronidase producing Escherichia coli and other enteric bacteria from ground beef

“…In a study carried out by Kalmus et al (2011) to evaluate antibiotic resistance of E. coli, ampicillin, streptomycin and tetracycline resistance were observed in 24.3, 15.6 and 13.5%, respectively, among the E. coli isolates. While examining the hygienic and sanitary quality of pasteurized cow's milk, E. coli was identified in 77.05% of the samples and the highest rates of resistance to antimicrobial agents were obtained for ampicillin (19.2%), cephalothin (18.9%) and tetracycline (17.1%) (Zanella et al, 2010). E. coli and other coliforms recovered from humans and animals had antibiotic resistance and several species were resistant to many antimicrobial agents commonly used in human and veterinary medicine (Greeson et al, 2013).…”