Occurrence and characteristics of extended-spectrum-β-lactamase- and AmpC-producing clinical isolates derived from companion animals and horses

Dierikx, Cindy; Duijkeren, Engeline van; Schoormans, A.; Essen-Zandbergen, Alieda van; Veldman, Kees; Kant, A.; Huijsdens, Xander W.; Zwaluw, Kim van der; Wagenaar, Jaap A.; Mevius, Dik

doi:10.1093/jac/dks049

Cited by 212 publications

(175 citation statements)

References 26 publications

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“…Microbiological investigation revealed isolation of strains of Staphylococcus aureus, Pseudomonas aeruginosa, E.coli, Klebsiella spp., Protius, Bacillus, Clostridium, Streptococcus, Micrococcus and Fusobacterium from different wounds from various species. Our result of bacterial isolation is in support with earlier reports of other workers (Goldstein, 1992;Kelly et al, 1992;Jousmies-Somer et al, 1995;Qureshi et al, 2002;Dierikx et al, 2012;Escobar et al, 2013). In the present study performed over animals for wounds among significant isolates Staphylococcus aureus was the most common isolate (36.22%) followed by E. coli from 34.59 % cases and showed different pattern of sensitivity as evident from the Table 2 and 3.…”

Section: Discussionsupporting

confidence: 92%

Bacterial Etiology of Skin and Wound Infections along with Antibiogram Profiling in Reference to Emerging Antibiotic Resistance in Animals

Tiwari¹,

Yadav²,

Singh³

et al. 2015

Adv. Anim. Vet. Sci.

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| The present study demonstrated bacterial etiology of skin affections and their antibiogram profiling of different animal species of various age groups. A total of 255 samples from cattle, buffalo, dogs, goats, sheep, camel and horses were collected over a period of two years (2012)(2013)(2014) from Mathura, (U.P.) India, and nearby surrounding areas. Clinical samples were collected from variety of skin disorders, subjected to laboratory isolation and identification as per standard protocols. The bacterial infection, Staphylococcus aureus, was the most common isolate (36.22 %), followed by E. coli (34.59 %), Pseudomonas (20.54), Bacillus (16.21), Klebsiella (12.43), Micrococcus spp (8.11 %), Streptococcus pyogens (7.56), Proteus (6.49), Clostridium (3.78), Gram negative non-lactose fermenter (2.7%), Gram positive non-spore producing bacilli (2.16) and Fusobacterium (0.54 %). Bacterial isolates obtained were subjected to in vitro antibiotic sensitivity testing by disc diffusion method against 23 commercially available antimicrobial discs. Results of the current study revealed maximum sensitivity to gatifloxacin (94.03%), amikacin (85.7%), mild sensitivity to sparfloxacin (44.85%) and gentamicin (43.48%); emergence of multi-drug resistant bacterial strains of Staphylococcus aureus, Pseudomonas aeruginosa, E.coli appeared to be an important finding. Present study is intended to document the complex microbial inhabitants of wounds from animals and their antimicrobial sensitivity pattern and strongly recommended the use of antimicrobial sensitivity testing to know the rapidly changing pattern of antibiotic activity. The study will further aid to the existing literature for planning new alternative therapeutic strategies against rising global health havoc of multidrug resistance.

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Section: Discussionsupporting

confidence: 92%

Bacterial Etiology of Skin and Wound Infections along with Antibiogram Profiling in Reference to Emerging Antibiotic Resistance in Animals

Tiwari¹,

Yadav²,

Singh³

et al. 2015

Adv. Anim. Vet. Sci.

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“…A survey carried out in Netherlands in companion animals and horses demonstrated a 2% prevalence of ESBL and AmpC-producing isolates. The same study identified, ESBL producing S. enterica, Proteus miriabilis and Enterobacter cloaceae from urinary, wound, respiratory, abdominal and bone infections (Dierikx et al, 2012). In Germany from six nosocomially infected dogs, OXA-48 type Carbopenamases producing E. coli and K. pneumonia were isolated (Stolle et al, 2013) and in France CTX-M-15 generating K. pneumoniae have been detected from urinary tract infections of dogs and cats (Haenni et al, 2012).…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 83%

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Milton¹

2015

Adv. Anim. Vet. Sci.

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| Nosocomial, or hospital-acquired, infections are well-thought-out to be the most common problem affecting hospitalized human patients as well as veterinary patients. These are of great consequence in veterinary medicine and can have considerable adverse effects on the individual patient as well as on the veterinary hospital as a whole. Different infectious agents like MRSA, Cl. difficile, MDR E. coli, Salmonella spp. and many more are of prodigious significance in causing NIs. A surveillance and control measure for these NIs in veterinary settings needs to be addressed in future. This review consolidates some Nosocomial infections and the strategy for its surveillance in veterinary practice.

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“…In farm animals, the use of antibiotics that are critically important in human medicine could result in the emergence of new forms of multi-resistant bacteria that infect people. Some new strains of multi-resistant foodborne bacteria, such as Campylobacter, Salmonella and Escherichia coli that produce the ESBL and/or AmpC enzymes that inactivate nearly all beta-lactam antibiotics and the critically important 3rd and 4th generation cephalosporins, have been isolated from meat and faecal samples of farm animals (Dierikx et al, 2012;Iweriebor et al,. 2015b).…”

Section: Acquired Resistance To Vancomycin Is Mediated By Various Genmentioning

confidence: 99%

Macrolide, glycopeptide resistance and virulence genes in Enterococcus species isolates from dairy cattle

Iweriebor

Chikwelu

Okoh

2016

Journal of Medical Microbiology

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The genus Enterococcus is known to possess the capacity to acquire and disseminate antimicrobial resistant determinants alongside the ability to produce various virulence genes that enables it to establish infections. We assessed the prevalence and antibiogram profiles of Enterococcus spp. in faecal samples of dairy cattle. Faecal swab samples were collected from 400 dairy cattle from two commercial cattle farms in two rural communities in the Eastern Cape, South Africa. Confirmation of enterococci isolates was carried out by PCR targeting of the tuf gene. Species delineation was by species-specific primers targeting the superoxide dismutase (sodA) gene in a multiplex PCR assay. Isolates were screened for the presence of the following virulence genes (ace, gelE, esp, efaA, cylA and hylE) and antimicrobial resistance determinants to erythromycin, vancomycin and streptomycin were evaluated molecularly. A total of 340 isolates were confirmed as belonging to the genus Enterococcus. Species distribution among the isolates consisted of Enterococcus faecium (52.94 %) and Enterococcus durans (23.53 %) in preponderance compared to the three other species, namely Enterococcus faecalis (8.8 %), Enterococcus hirae (8.6 %) and Enterococcus casseliflavus (5.9 %). All were resistant to vancomycin, while 99 % showed resistance to aminoglycoside and 94 % to macrolide. Three virulence genes (ace, gelE and esp) were detected in almost all the confirmed isolates. The resistance determinants vanB (19.7 %), vanC1 (25 %), vanC2/3 (26.3 %) ermB (40.29 %) and strA (50.88 %) were detected among the isolates. A high prevalence of multidrug-resistant enterococci isolates was detected in this study and the genetic repertoire to survive in the presence of antimicrobial agents was present in these organisms. INTRODUCTIONMembers of the genus Enterococcus are known to be abundantly present in the intestinal tracts of humans and warmblooded animals, as well as in water and soil environments. These bacteria have become notorious recently because of their ability to cause nosocomial infections, especially among both immunocompromised patients and elderly people. Pathogenic enterococci have been reported to harbour a pathogenicity island that encodes for aggregation substance (AS), gelatinase, extracellular surface proteins (esp), cytolysin, hyaluronidase and other proteins (Giridhara Upadhyaya et al., 2009;Shankar et al., 2002). These virulence factors enable enterococci to cause tissue damage and inflammation.Besides their possession of several virulence factors, members of the genus Enterococcus also have inherent capacity to accumulate and disseminate antimicrobial resistance determinants. Enterococci, like other bacteria are capable of developing strategies to enhance their survival in adverse situations (Garcia-Migura et al., 2005). They have emerged as an important opportunistic pathogen causing life-threatening infections in patients, alongside the fact that they have developed remarkable resistance to a battery of antimicrobial agents belo...

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Occurrence and characteristics of extended-spectrum-β-lactamase- and AmpC-producing clinical isolates derived from companion animals and horses

Cited by 212 publications

References 26 publications

Bacterial Etiology of Skin and Wound Infections along with Antibiogram Profiling in Reference to Emerging Antibiotic Resistance in Animals

Bacterial Etiology of Skin and Wound Infections along with Antibiogram Profiling in Reference to Emerging Antibiotic Resistance in Animals

Nosocomial Infections and their Surveillance in Veterinary Hospitals

Macrolide, glycopeptide resistance and virulence genes in Enterococcus species isolates from dairy cattle

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