Occurrence and Characterization of Rotavirus A in Broilers, Layers, and Broiler Breeders from Brazilian Poultry Farms

Beserra, Laila Andreia Rodrigues; Barbosa, Bruna Rocha Passos; Bernardes, Nara Thiers Cacciatori Galleti; Brandão, Paulo Eduardo; Gregori, Fábio

doi:10.1637/10626-080513-resnote.1

Cited by 16 publications

(17 citation statements)

References 17 publications

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“…Highest prevalence has been observed (40%) in 12-day-old birds while the lowest was observed in birds aging 22 days (6%) (Ahmed & Ahmed 2006;Karim et al 2007). A recent study in Brazil revealed 53.8% prevalence of rotavirus from broilers, layers and broiler breeders (Beserra et al 2014). In countries like Bangladesh, RV disease is reported as 0.9% of diarrheic broilers (Ahmed 2013).…”

Section: Epidemiologymentioning

confidence: 99%

Avian rotavirus enteritis – an updated review

Dhama

Saminathan

Karthik

et al. 2015

Veterinary Quarterly

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Section: Epidemiologymentioning

confidence: 99%

Avian rotavirus enteritis – an updated review

Dhama

Saminathan

Karthik

et al. 2015

Veterinary Quarterly

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“…Dandar et al (2013) characterized divergent avian reovirus strain from a broiler chicken with central nervous system disease. For the first time, Beserra et al (2014) reported the occurrence of rotavirus group F in broiler from Brazil. Gyuranecz et al (2013) established worldwide phylogenetic relationship of avian poxviruses and provided novel insights into the complex relationship between avian poxviruses and their hosts.…”

Section: Classical Methods For Study Of Virus Diversitymentioning

confidence: 99%

Next generation sequencing technologies: Tool to study avian virus diversity

Kapgate¹,

Barbuddhe²,

Kumanan³

2015

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Summary. -Increased globalisation, climatic changes and wildlife-livestock interface led to emergence of novel viral pathogens or zoonoses that have become serious concern to avian, animal and human health. High biodiversity and bird migration facilitate spread of the pathogen and provide reservoirs for emerging infectious diseases. Current classical diagnostic methods designed to be virus-specific or aim to be limited to group of viral agents, hinder identifying of novel viruses or viral variants. Recently developed approaches of next-generation sequencing (NGS) provide culture-independent methods that are useful for understanding viral diversity and discovery of novel virus, thereby enabling a better diagnosis and disease control. This review discusses the different possible steps of a NGS study utilizing sequence-independent amplification, high-throughput sequencing and bioinformatics approaches to identify novel avian viruses and their diversity. NGS lead to the identification of a wide range of new viruses such as picobirnavirus, picornavirus, orthoreovirus and avian gamma coronavirus associated with fulminating disease in guinea fowl and is also used in describing viral diversity among avian species. The review also briefly discusses areas of viral-host interaction and disease associated causalities with newly identified avian viruses.

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“…No Brasil não existem estudos de genoma completo de AvRV e os dados acerca da diversidade genética dos mesmos é limitada, ficando restrito à caracterização dos genotipos G, P e E (ASANO et al, 2011;SILVA et al, 2011;SILVA et al, 2012;METTIFOGO et al, 2014;BESERRA et al, 2014a).…”

Section: Estrutura E Classificaçãounclassified

“…Foram descritos até o momento, sete genotipos G -G1, G5, G6, G8, G10, G11 e G19 -três genotipos P -P (ASANO et al, 2011;SILVA et al, 2012;BESERRA et al, 2014a). Está descrito ainda o genotipo E10, detectado em amostras de frango de corte dos estados do Pará e São Paulo (SILVA et al, 2011;METTIFOGO et al, 2014).…”

Section: Estrutura E Classificaçãounclassified

“…No Brasil, rotavírus do grupo A (RVA) foram primeiramente detectados, através das técnicas de PAGE e ELISA, em fezes de frango de corte com diarreia nos estados de Minas Gerais e Paraná (ALFIERI et al, 1989) e posteriormente descritos em amostras fecais de frango de corte, poedeiras e matrizes em estados das regiões norte, nordeste, centro-oeste, sudeste e sul do país (TAMEHIRO et al, 2003;VILLARREAL et al, 2006;RIOS et al, 2012;SILVA et al, 2013;METTIFOGO et al, 2014;BESERRA et al, 2014a), além de criações comerciais de perus (ASANO et al, 2011;MOURA-ALVAREZ et al, 2014) (McNULTY, 2008;TROJNAR et al, 2009;JOHNE et al, 2011;KATOOR et al, 2013;STUCKER et al, 2015;FALCONE et al, 2015). No Brasil, RVD foi primeiramente detectado na região norte (BEZERRA et al, 2012;BEZERRA et al, 2014) e em seguida nos estados de São Paulo, Paraná e Goiás, em amostras de frango de corte, matrizes e poedeiras comerciais sem sintomas de diarreia (BESERRA et al, 2015).…”

Section: Introductionunclassified

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<b>Análise multigênica de rotavírus do grupo A em aves de criações comerciais brasileiras</b>

Beserra¹

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Rotaviruses are members of the Reoviridae family and they are a common cause of acute diarrhea in several mammalian and avian species. They are non-enveloped icosahedral particles and its genome comprises 11 segments of double-stranded RNA, which encodes six structural proteins (VP1-4, VP6-7) and six nonstructural proteins (NSP1-6). The objective of this study was to characterize the RVA nonstructural and structural proteins coding genes (NSP1-NSP5, VP1-VP4, VP6 and VP7) from fecal samples from avian farms (broiler breeders, poultry, laying hens, and grandparents) raised in Brazilian commercial farms from 12 states, followed by recombination and selection pressure analysis from samples defined here. A total of 226 fecal samples were screened using a RT-PCR technique targeting the amplification of the VP6 and NSP5. The frequency of occurrence, using these techniques, ranging from 9.7% to 18,14%, respectively; and from these, ten samples were further processed with specific primers to amplify the remaining genes, followed by respective nucleotide sequencing of the amplicons and phylogeny based on method maximum likelihood, as substitutions models GTR (NSP1-3, VP1-3, VP4, VP6, VP7) and HKY (NSP4, NSP5) and 1.000 bootstrap repetitions. Partial nucleotide sequences of VP1-4, VP6-7, and NSP1-4, and complete from NSP5, were obtained in this study. The phylogenetic trees depicted that the ten Brazilian rotavirus strains segregated with previous avian RVA described elsewhere. Two avian genotype constellations have been characterized here: G19-P[31]-I11-R6-C6-M7-A16-N6-T8-E10-H8, and G19-P[31]-I4-R4-C4-M4-A16-N4-T4-E4-H4. These genotypes are typically found in avian species, although when analyzed together, this is the first report of such constellations. Recombination events were observed in NSP2, VP1, VP3, and VP7 coding genes. At least on positive selected site was observed in NSP1, VP2, and VP3 genes. This study provides a better understanding of rotavirus epidemiology, by the definition of genetic variability of circulating strains.

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Occurrence and Characterization of Rotavirus A in Broilers, Layers, and Broiler Breeders from Brazilian Poultry Farms

Cited by 16 publications

References 17 publications

Avian rotavirus enteritis – an updated review

Avian rotavirus enteritis – an updated review

Next generation sequencing technologies: Tool to study avian virus diversity

<b>Análise multigênica de rotavírus do grupo A em aves de criações comerciais brasileiras</b>

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