Occurrence and detection of little cherry virus 1, little cherry virus 2, cherry green ring mottle virus, cherry necrotic rusty mottle virus, and cherry virus A in stone fruit trees in Poland

Komorowska, B.; Hasiów‐Jaroszewska, Beata; Czajka, Agnieszka

doi:10.4149/av_2020_112

Cited by 4 publications

(1 citation statement)

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“…Many viruses and viroids infecting stone fruits—including peach, plum, apricot, and cherry—are being identified ( Jo et al, 2018b ; Kinoti et al, 2020 ; Komorowska, Hasiów-Jaroszewska & Czajka, 2020 ). Of the stone fruit trees, plum trees could be the second-most important trees for providing fresh fruits during the summer season in Korea.…”

Section: Discussionmentioning

confidence: 99%

Identification of viruses infecting six plum cultivars in Korea by RNA-sequencing

Choi

Lian

et al. 2020

PeerJ

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Background Plums are a kind of stone fruit, a category that includes peaches, cherries, apricots, and almonds. In Korea, Japanese plum trees are usually cultivated as they best suit the climate. To date, there have been few studies in Korea on viruses infecting plum trees compared to those infecting peach trees. Methods To identify viruses and viroids infecting plum trees, we collected leaf samples from six different plum cultivars and subjected them to RNA-sequencing (RNA-seq). Six different plum transcriptomes were de novo assembled using the Trinity assembler followed by BLAST searching against a viral reference database. Results We identified hop stunt viroid (HSVd) and six viruses, including apple chlorotic leaf spot virus (ACLSV), little cherry virus-1 (LChV-1), peach virus D (PeVD), peach leaf pitting-associated virus (PLPaV), plum bark necrosis stem pitting-associated virus (PBNSPaV), and prunus necrotic ringspot virus (PNRSV), from six plum cultivars by RNA-seq. RT-PCR confirmed the infection of HSVd and three viruses—ACLSV, PBNSPaV, and PNRSV—in plum trees. However, RT-PCR demonstrated that plum trees in this study were not infected by LChV-1, PeVD, or PLPaV. It is likely that the three viruses LChV-1, PeVD, and PLPaV as identified by RNA-seq were contaminants from other peach libraries caused by index misassignment, which suggests that careful confirmation by other methods should be carried out in next-generation sequencing (NGS)-based virus identification. Taken together, we identified a viroid and three viruses infecting plum trees in Korea.

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Section: Discussionmentioning

confidence: 99%