Hematology can be a useful tool for monitoring health status, detecting illness, and following the progress of disease and response to therapy. Despite advances in fish medicine in recent years, interpretation of fish hematology often is hampered by a lack of meaningful reference values and the bewildering diversity of fish species. Hematology is the science of studying the anatomical, physiological, and pathological aspects of blood. This study gives valuable information on fish Tilapia zillii, Hematological indices (RBC, WBC, Hct, Hb, MCV, MCH, MCHC, GRA, MON, LYM, and PLT) were measured in blood in samples from 30 adult fish captured from (Ain Elmajdob lake), Benghazi city. Statistical analysis revealed that white blood cells were found depending on the length and weight of Tilapia zillii as studied by regression of white blood cells on length and weight of Tilapia zillii. Moreover, Lymphocytes and Monocytes increased with the increase in weight, as studied by regression of Lymphocytes and Monocytes on the length and weight of Tilapia zillii. Nevertheless, not significant in Red blood cells, Hemoglobin, Haematocrit, Mean corpuscular volume, Mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelets.