Occurrence and genetic characteristics of mcr-1-positive colistin-resistant E. coli from poultry environments in Bangladesh

Amin, Mohammed Badrul; Sraboni, Ajrin Sultana; Hossain, Muhammed; Roy, Subarna; Mozmader, Tim Amin Uddin; Unicomb, Leanne; Rousham, Emily K.; Islam, Mohammad Aminul

doi:10.1016/j.jgar.2020.03.028

Cited by 37 publications

(31 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the true occurrence of mcr-1 gene in poultry isolates is 27.6%. Similar result was reported by a study in Iran [50] while a study from Bangladesh reported a higher prevalence (94%) of colistin resistance in ESBLs E. coli isolates in poultry [51]. In this study, among 12 colistin resistant isolates, 11 (91.6%) were mcr-1 positive.…”

Section: Discussionsupporting

confidence: 90%

Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

et al. 2020

View full text Add to dashboard Cite

Background Plasmid-mediated resistance to the last-resort drugs: carbapenems and colistin is an emerging public health threat. The studies on the prevalence and co-expression of resistant genes among livestock and human pathogens are rare in Nepal. This is the first study in Nepal exploring the prevalence and co-existence of colistin resistance gene, mcr-1 along with carbapenemase resistance gene, OXA-48 in Escherichia coli isolated from poultry and clinical specimens. Methods A total of 240 rectal swabs from chickens of five different poultry farms of Kathmandu valley and 705 mid-stream urine samples from human subjects attending Kantipur Hospital, Kathmandu were collected between August, 2018 and March, 2019. Rectal swabs and urine specimens were cultured. E. coli isolated from the specimens were screened for antimicrobial susceptibility testing (AST) using disk diffusion method’. Minimum inhibitory concentration (MIC) of colistin was determined by agar dilution method using 0.5 µg/ml to 32 µg/ml. The E. coli isolates were first screened for mcr-1 followed by screening for OXA-48 genes using conventional Polymerase chain reaction (PCR). Results Of the total samples analyzed, E. coli was isolated from 31.7% (76/240) of poultry and 7.9% (56/705) of clinical specimens. In AST, 80% (61/76) of E. coli from poultry and 79% (44/56) from clinical specimens were MDR. The phenotypic prevalence of colistin resistance in poultry specimens were 31.6% (24/76) and clinical specimens were 21.4% (12/56). In PCR assay, 27.6% (21/76) of poultry and 19.6% (11/56) of clinical isolates had colistin resistant mcr-1 gene. MICs value of E. coli isolates ranged from 4 to 32 (µg/ml) in both clinical and poultry isolates. Prevalence of co-existing carbapenem resistance gene, OXA-48, among colistin resistant mcr-1 positive isolates was 38% (8/21) in poultry specimens and 18.2% (2/11) in clinical specimens. Conclusions The high prevalence of colistin and carbapenem resistant genes, and their co-existence in plasmid DNA of E. coli isolates in this study suggests the possible spread to other animal, human and environmental pathogens. Molecular methods in addition to the conventional diagnostics in laboratories can help in early diagnosis, effective management and control of their potential transmission.

show abstract

Section: Discussionsupporting

confidence: 90%

Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Overall, the high frequency of mcr-1 gene detected in this study is still the exception, and much higher than previously reported studies from Germany (3.8%) 25 , China (1% or less) and other countries 26 – 28 . Also, this study shows high MIC against colistin (≥ 8 mg/L) in more than 50% of the mcr-1 carrying E. coli which is very similar with another study in Bangladesh 29 , and the overall MIC is higher than other studies in some European countries 25 , 30 , 31 . It suggests that the commensal E. coli population in livestock in countries with a high and unregulated use of colistin constitutes a hotspot for selection for carriage of transferrable resistance.…”

Section: Discussionsupporting

confidence: 89%

High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Ahmed

Das

Islam

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Colistin is a last-resort antimicrobial used for the treatment of human infections caused by multidrug-resistant Gram-negative bacteria. However, colistin is still widely used in intensive poultry production in Bangladesh. We aimed to investigate the dynamics and genetic diversity of colistin-resistant commensal Escherichia coli from broiler chickens. A total of 1200 E. coli strains were characterized from 20 broiler farms at three-time points along the production period. All strains were screened for mcr-1 to mcr-5 genes by a multiplex PCR, and their genetic diversity was measured by repetitive extragenic palindromic (REP)-PCR fingerprinting. Genomic diversity and characterization were performed by whole genome sequencing (WGS). Twenty-five percent of the commensal E. coli strains harbored mcr-1 genes. Frequency of mcr-1 gene detection correlated positively (odds ratio 1.71; 95% CI 0.96–3.06; p = 0.068) with the use of colistin in poultry flocks. REP-PCR profiles and WGS analysis showed diverse E. coli population carrying multiple antimicrobial resistance genes. Phylogenetic comparison of mcr-1-bearing strains recovered from this study with a global strain collection revealed wide phylogenetic relationship. This study identified a high prevalence of mcr-1 gene among genetically diverse E. coli populations from broiler chickens in Bangladesh suggesting a massive horizontal spread of mcr-1 rather than by clonal expansion.

show abstract

“…AMR prevalence in Bangladesh has been reported widely in animal husbandry, environment, and aquaculture [68][69][70]. The presence of various β-lactamase genes, extended-spectrum β-lactamase (ESBL), and different types of mobile colistin resistance (mcr) genes were found in Bangladeshi veterinary and environmental sources [71][72][73][74]. Antimicrobial-resistant bacteria and antibiotic resistance genes (ARGs) have been reported extensively in hospital and community-acquired infection [75][76][77][78], gastroenteritis [79,80], urinary tract infection (UTI) [81,82], respiratory tract infection [83], skin and tissue infection [75], blood-borne infections [84].…”

Section: A Snapshot Of Amr Surveillance Programs and The Epidemiologymentioning

confidence: 99%

Surveillance of antimicrobial resistance in low- and middle-income countries: a scattered picture

Iskandar

Molinier

Hallit

et al. 2021

Antimicrob Resist Infect Control

232

144

View full text Add to dashboard Cite

Data on comprehensive population-based surveillance of antimicrobial resistance is lacking. In low- and middle-income countries, the challenges are high due to weak laboratory capacity, poor health systems governance, lack of health information systems, and limited resources. Developing countries struggle with political and social dilemma, and bear a high health and economic burden of communicable diseases. Available data are fragmented and lack representativeness which limits their use to advice health policy makers and orientate the efficient allocation of funding and financial resources on programs to mitigate resistance. Low-quality data means soaring rates of antimicrobial resistance and the inability to track and map the spread of resistance, detect early outbreaks, and set national health policy to tackle resistance. Here, we review the barriers and limitations of conducting effective antimicrobial resistance surveillance, and we highlight multiple incremental approaches that may offer opportunities to strengthen population-based surveillance if tailored to the context of each country.

show abstract

Occurrence and genetic characteristics of mcr-1-positive colistin-resistant E. coli from poultry environments in Bangladesh

Cited by 37 publications

References 36 publications

Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

Plasmid mediated colistin resistant mcr-1 and co-existence of OXA-48 among Escherichia coli from clinical and poultry isolates: first report from Nepal

High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Surveillance of antimicrobial resistance in low- and middle-income countries: a scattered picture

Contact Info

Product

Resources

About