High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Ahmed, Shahana; Das, Tridip; Islam, Zohorul; Herrero-Fresno, Ana; Biswas, Paritosh Kumar; Olsen, John Elmerdahl

doi:10.1038/s41598-020-75608-2

Cited by 38 publications

(38 citation statements)

References 74 publications

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“…Various species of Enterobacteriaceae carrying mcr-1 plasmid-mediated resistance have been identified from humans, animals and environments in Asia, Europe, Africa, North America and South America [ 51 ]. Furthermore, the proportion of mcr-1 carriers among colistin-resistant isolates was higher in food-producing animals investigated in Italy [ 52 ], Germany [ 53 ], UK [ 54 ], Poland [ 55 ], Bangladesh [ 56 ], India [ 57 ], Pakistan [ 58 ], and South Korea [ 59 ]. Interestingly, the detection of the colistin resistance mcr-1 gene associated with the risk of subsequent transmission to unexposed human populations in southern Vietnam was also reported [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Uddin

Hossain

Hasan

et al. 2021

Animals

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Colistin (polymyxin E) is widely used in animal and human medicine and is increasingly used as one of the last-resort antibiotics against Gram-negative bacilli. Due to the increased use of colistin in treating infections caused by multidrug-resistant Gram-negative bacteria, resistance to this antibiotic ought to be monitored. The study was undertaken to elucidate the molecular mechanisms, genetic relationships and phenotype correlations of colistin-resistant isolates. Here, we report the detection of the mcr-1 gene in chicken-associated Salmonella isolates in Bangladesh and its in-silico functional analysis. Out of 100 samples, 82 Salmonella spp. were isolated from chicken specimens (liver, intestine). Phenotypic disc diffusion and minimum inhibitory concentration (MIC) assay using different antimicrobial agents were performed. Salmonella isolates were characterized using PCR methods targeting genus-specific invA and mcr-1 genes with validation for the functional analysis. The majority of the tested Salmonella isolates were found resistant to colistin (92.68%), ciprofloxacin (73.17%), tigecycline (62.20%) and trimethoprim/sulfamethoxazole (60.98%). When screened using PCR, five out of ten Salmonella isolates were found to carry the mcr-1 gene. One isolate was confirmed for Salmonella enterica subsp. enterica serovar Enteritidis, and other four isolates were confirmed for Salmonella enterica subsp. enterica serovar Typhimurium. Sequencing and phylogenetic analysis revealed a divergent evolutionary relationship between the catalytic domain of Neisseria meningitidis lipooligosaccharide phosphoethanolamine transferase A (LptA) and MCR proteins, rendering them resistant to colistin. Three-dimensional homology structural analysis of MCR-1 proteins and molecular docking interactions suggested that MCR-1 and LptA share a similar substrate binding cavity, which could be validated for the functional analysis. The comprehensive molecular and in-silico analyses of the colistin resistance mcr-1 gene of Salmonella spp. of chicken origin in the present study highlight the importance of continued monitoring and surveillance for antimicrobial resistance among pathogens in food chain animals.

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Section: Discussionmentioning

confidence: 99%

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Uddin

Hossain

Hasan

et al. 2021

Animals

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“…Despite the low prevalence of the mcr-1 gene, the frequent contamination of poultry carcasses with intestinal contents during the slaughter process has been reported 24) . A study conducted in Bangladesh found high prevalence (25%) of the mcr-1 gene in E. coli isolated from broiler chickens, and this finding was associated with high CST usage for treatment and prophylaxis purposes 32) . In addition, a survey in the Netherlands detected the mcr-1 gene (8%) in chicken 15) .…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Colistin-Resistant Bacteria among Retail Meats in Japan

et al. 2021

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Colistin (CST) is considered the last resort for the treatment of infectious diseases due to multidrug-resistant bacteria. Since the mcr-1 gene has been reported in Enterobacteriaceae isolated from food, animals, and humans in China, the prevalence of CST-resistant bacteria has been of great concern. Here, we investigated the prevalence of CST resistance and plasmid-mediated colistin-resistance genes (mcr) in gram-negative bacteria isolated among retail meats in Japan. CST-resistant bacteria were isolated from 310 domestic retail meats (103 chicken meat, 103 pork, and 104 beef) purchased between May 2017 and July 2018 from retail shops in Japan using CST-containing media and antimicrobial susceptibility testing. The mcr gene was investigated in isolates with a CST minimum inhibitory concentration of ≥1 μg/mL. Excluding the intrinsically CST-resistant isolates, CST-resistant bacteria were isolated from 39 of the total chicken meats (37.9%), 19 of the pork samples (18.4%), and 18 of the beef samples (17.3%). A total of 459 isolates were identified, out of which 99 were CST-resistant. CST resistance (resistance breakpoints: Aeromonas, >4 μg/mL; others, >2 μg/mL) was found in

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“…Interestingly, the use of colistin in broilers and pigs has been shown to lead to the emergence of colistin-resistant E. coli isolates whereas initially, those isolates were sensitive to colistin [ 5 , 16 ]. Colistin-resistant isolates (positive mcr-1 ) are more likely to be found in samples of animal origin rather than human origin [ 17 ]. However, their prevalence in sewage treatment plants is yet mainly unknown [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran

Nikkhahi

Robatjazi

Niazadeh

et al. 2021

New Microbes and New Infections

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Currently, few studies have investigated the mechanisms of resistance to colistin in Iran. The aim of this study was to investigate mcr -harbouring Escherichia coli dissemination in livestock and sewage in Iran. A total of 115 samples from cows ( n = 38), chickens ( n = 47) and urban sewage samples ( n = 30) were collected. The presence of genes including mcr1–6 and ampC β-lactamase ( bla MOX , bla CIT , bla DHA , bla ACC , bla EBC , bla FOX ) for colistin-resistant isolates was investigated by multiplex PCR method. Genetic association of colistin-resistant strains was also evaluated by ERIC PCR. Sixty-five isolates were identified as E. coli . Meaningless were resistant to colistin. The highest (26.1%) and lowest (3.07%) resistance were shown to ampicillin and meropenem respectively. Among the three colistin-resistant isolates, 2 (66%) were multidrug resistant, with one of them being mcr-1 positive and the other one positive for DHA ampC β-lactamase gene. No mcr2–6 genes were found. Minimum inhibitory concentration of mcr -producing isolate was 4 mg/L by microbroth dilution. This study reports, first the detection of mcr-1 in E. coli from farm animals in Iran, a finding that is indicative of a global distribution of this plasmidic element and threatning the use of colistin as a last resort antibiotic. No clonal relationship was observed between the colistin-resistant E. coli isolates by ERIC-PCR. Monitoring the presence of these strains in animal sources help as to controlling the spread of resistance genes from animal to human is vital.

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High prevalence of mcr-1-encoded colistin resistance in commensal Escherichia coli from broiler chicken in Bangladesh

Cited by 38 publications

References 74 publications

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Multidrug Antimicrobial Resistance and Molecular Detection of mcr-1 Gene in Salmonella Species Isolated from Chicken

Prevalence of Colistin-Resistant Bacteria among Retail Meats in Japan

First detection of mobilized colistin resistance mcr-1 gene in Escherichia coli isolated from livestock and sewage in Iran

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