Occurrence and genetic diversity of Arcobacter spp. in a spinach-processing plant and evaluation of two Arcobacter-specific quantitative PCR assays

Hausdorf, Lena; Neumann, Mark E; Bergmann, Ingo; Sobiella, Kerstin; Mundt, K.; Fröhling, Antje; Schlüter, Oliver; Klocke, Michael

doi:10.1016/j.syapm.2013.02.003

Cited by 52 publications

(56 citation statements)

References 42 publications

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“…A. butzleri demonstrated the ability to survive during the production process and shelf life of WB mozzarella cheese (11), and raw milk cheeses may be considered a potential source of human infection even though A. butzleri has not been isolated from dairy products. A. butzleri contamination of food processing plants may be an additional source of food contamination, as demonstrated in poultry slaughterhouses and spinach processing plants (12)(13)(14)(15)(16)(17). To our knowledge, no study has hitherto investigated the presence of Arcobacter spp.…”

mentioning

confidence: 99%

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Giacometti

Lucchi

Manfreda

et al. 2013

Appl Environ Microbiol

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bThe present study aimed to investigate the presence, distribution, and persistence of Arcobacter spp. in an artisanal dairy plant and to test the isolates to determine their different genotypes in the processing plant and in foods. Samples were collected in an artisanal cheese factory on four occasions between October and December 2012. Food samples (raw milk, ricotta cheese, mozzarella cheese, and conditioning liquid), water samples, and environmental samples were analyzed by the culture method; isolates were identified by multiplex PCR and genotyped by pulsed-field gel electrophoresis (PFGE) analysis. Arcobacter butzleri was isolated from 29 out of 59 samples (46.6%), 22 of which were from environmental samples and 7 of which were from food samples. Cluster analysis divided the strains into 47 PFGE patterns: 14 PFGE clusters and 33 unique types. Our findings indicate that the plant harbored numerous A. butzleri pulsotypes and that the manual cleaning and sanitation in the studied dairy plant do not effectively remove Arcobacter. The recurrent isolation of A. butzleri suggests that the environmental conditions in the dairy plant constitute a good ecological niche for the colonization of this microorganism. In some cases, the presence of indistinguishable strains isolated from the same facilities on different sampling days showed that these strains were persistent in the processing environment. Interest in arcobacters in veterinary and human public health has increased from the first report of the isolation of arcobacters from food of animal origin; since then, studies worldwide have reported the occurrence of arcobacters on food and in food production animals and have highlighted possible routes of transmission, especially for Arcobacter butzleri, to the human population (1).Arcobacter butzleri is the most important and prevalent species of the genus and has been classified as a serious hazard to human health by the International Commission on Microbiological Specifications for Foods (2) and as a significant zoonotic pathogen (3). A few surveys have investigated the presence of Arcobacter spp. in bulk tank raw cow's milk, reporting prevalence rates of 46% in Northern Ireland (4), 5.8% in Malaysia (5), and 48% in Italy (6). In Italy, A. butzleri was isolated in fecal samples and in-line milk filters on a water buffalo (WB) dairy farm (7,8). Consumption of Arcobacter-contaminated food or water is regarded as the most probable route of transmission to humans and animals (9) following fecal contamination during the various stages of production processes (10). In the Mediterranean countries, there is a widespread tradition of raw milk cheese production, including, particularly in Italy, WB cheeses. A. butzleri demonstrated the ability to survive during the production process and shelf life of WB mozzarella cheese (11), and raw milk cheeses may be considered a potential source of human infection even though A. butzleri has not been isolated from dairy products. A. butzleri contamination of food processing plants may...

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confidence: 99%

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Giacometti

Lucchi

Manfreda

et al. 2013

Appl Environ Microbiol

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“…Recreational water and drinking water are both likely sources of exposure along with irrigation water, as Arcobacter appear to survive well in water (Van Driessche and Houf, 2008). Irrigation or washing of crops meant for raw consumption (vegetables) with water containing Arcobacter is a potential transmission route (Hausdorf et al, 2013;. Arcobacter has been found not only in raw food products but also in meals at popular restaurants in Bangkok where it was determined that the risk of exposure per consumed meal was 13%, and it was up to 75% in the case of 10 meals or more and references therein).…”

Section: Routes Of Transmissionmentioning

confidence: 99%

“…This is particularly common in intensive farming operations where water is consumed by the animals or used to wash animal carcasses or vegetable produce (Hausdorf et al, 2013).…”

Section: Watermentioning

confidence: 99%

“…Various species of Arcobacter have been detected in a spinach processing plant at multiple locations (Hausdorf et al, 2013). Arcobacter was detected in the process water and wash basins and the known potential pathogenic species A. butzleri was cultured from washed and blanched spinach, showing the potential for leafy green vegetables to be implicated in gastrointestinal disease.…”

Section: Irrigation Water and On Cropsmentioning

confidence: 99%

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Arcobacter

Banting¹,

Salvat²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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“…These species have been isolated in particular from poultry (Atabay et al 1998;Amare et al 2011), meat (Van Driessche andHouf 2007;Pejchalova et al 2008), faeces of humans and animals suffering from gastrointestinal tract diseases, and aborted cattle foetuses (A. butzleri, A. cryaerophilus, A. skirrowii, A. cibarius, A. thereius, and A. trophiarum). Recently, however, these species have also very often been found in samples from wastewater (Gonzalez and Ferrus 2011;Hausdorf et al 2013) and marine environments (A. marinus, A. molluscorum, A. mytili, A. ellisii, A. bivalviorum, and A. venerupis) (Fera et al 2004;Collado et al 2009;Levican et al 2012;Levican et al 2013). Other representatives include A. defluvii from sewage water (Collado et al 2010), A. nitrofigilis and A. halophilus from salt marshes (Levican et al 2012), and the newly classified A. cloacae, A. suis (Levican et al 2013), and A. anaerophilus (Jyothsna et al 2013).…”

mentioning

confidence: 99%

Presence of Arcobacter species in pet cats and dogs in the Czech Republic

Pejchalová¹,

Žabčíková²,

Šilhová³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:This study was conducted to evaluate the occurrence of the genus Arcobacter in cats and dogs in the Czech Republic. These animals may be carriers of the bacteria and potential sources of human infection. Oral smears were collected from animals using smear swabs and brushes. Based on previous studies, commercially available DNA kits were used for DNA isolation. Samples were analysed using polymerase chain reaction (PCR) and evaluated using gel electrophoresis. Overall, 178 oral smears were tested, of which 108 were from dogs and 70 were from cats. Out of all smears, five were positive, of which four samples were from dogs and one from a cat. In all five positive cases, PCR confirmed the presence of Arcobacter butzleri. In follow-up sampling, the presence of Arcobacter butzleri was demonstrated in two samples from a dog.

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Occurrence and genetic diversity of Arcobacter spp. in a spinach-processing plant and evaluation of two Arcobacter-specific quantitative PCR assays

Cited by 52 publications

References 42 publications

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Occurrence and Genetic Diversity of Arcobacter butzleri in an Artisanal Dairy Plant in Italy

Arcobacter

Presence of Arcobacter species in pet cats and dogs in the Czech Republic

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