Occurrence and infective potential of Colletotrichum gloeosporioides isolates associated to Citrus limon var Eureka

Patricia, Larralde-Corona Claudia; Socorro, Ramírez-González María del; Iván, R Vega-Valdez; Erika, De la Cruz-Arguijo; Carolina, De Santiago; Shirai, Kohji; Narváez-Zapata, José Alberto

doi:10.1016/j.btre.2021.e00651

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…The leaves (gently scraped surface using a razor blade) and young branches were also inoculated under the same conditions. The detached branches were subjected to 5–10 mm longitudinal wounds under the epidermal layer using a sterile scalpel, then inoculated with target isolates and fixed using Parafilm (Patricia et al., 2021; Riolo et al., 2021). The control treatment was inoculated with uncolonized PDA plugs and incubated under the same conditions.…”

Section: Methodsmentioning

confidence: 99%

“…The prominent citruses grown in Thailand include mandarin/tangerine (Citrus reticulata), pomelo (C. grandis or C. maxima), acid lime (C. aurantifolia), Persian lime (C. latifolia) and Thai/kaffir lime (C. hystrix) (Sethpakdee, 1997). The diversity of citrus fruit has made it a preferred host for various fungal species with differing aetiology (Cui et al, 2021;Patricia et al, 2021) and citrus crops are afflicted by many plant-pathogenic fungi, causing diseases and reducing their production. Hence, the study and identification of fungal diseases in citrus trees is a critical and key step for their disease management (Wang et al, 2021).…”

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confidence: 99%

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Characterization of Colletotrichum species obtained from citrus in northern Thailand and introducing a new host record for C. plurivorum

Najafiniya,

de Farias,

Armand

et al. 2024

Plant Pathology

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Citrus trees are affected by many plant‐pathogenic fungi. The present study aimed to characterize Colletotrichum species on citrus showing anthracnose symptoms, leaf and fruit spot and wither tip in northern Thailand using multilocus data and assay their pathogenicity. The surveys were carried out from December 2021 to January 2022 and 73 isolates were obtained from infected tissues. The fungal strains were identified based on morphological traits and were confirmed via amplification and sequencing of ITS, ACT, CHS‐1, GAPDH and TUB2. In all, 41 isolates were subjected to multilocus phylogeny. Forty of these isolates of Colletotrichum species belonged to the C. gloeosporioides species complex and one isolate belonged to the C. orchidearum species complex. Colletotrichum siamense, comprising 19 isolates, was the most dominant species associated with symptomatic citrus, followed by C. gloeosporioides (17 isolates), C. fructicola (three isolates), C. endophytica (one isolate) and C. plurivorum (one isolate). C. plurivorum is reported for the first time from citrus in the world. These isolates were obtained from mandarin (Citrus reticulata), pomelo (C. maxima), Persian lime (C. latifolia), Thai lime (C. hystrix) and acid lime (C. aurantifolia). The pathogenicity of the isolated fungi was tested through inoculation on branch, fruit and leaf using the detached method on mandarin, pomelo and Persian lime hosts, confirming Koch's postulate. In terms of disease severity, C. gloeosporioides and C. siamense were observed to be the most pathogenic species. This is the first pathogenic and molecular study of Colletotrichum species infecting citrus in northern Thailand.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Characterization of Colletotrichum species obtained from citrus in northern Thailand and introducing a new host record for C. plurivorum

Najafiniya,

de Farias,

Armand

et al. 2024

Plant Pathology

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“…The phytopathogenic fungal strains of Colletotrichum gloeosporioides (AL-13) [15], Fusarium sp. (AL-21), and Penicillium digitatum (AL-38) were isolated from infected lemons in a packing facility from Tamaulipas (Mexico).…”

Section: Fungal Strainsmentioning

confidence: 99%

Yeast Mixtures for Postharvest Biocontrol of Diverse Fungal Rots on Citrus limon var Eureka

Edward-Rajanayagam,

Narváez-Zapata,

Ramírez-González

et al. 2023

Horticulturae

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Mexico is among the most important citrus fruit producers in the world. However, during storage, several problems related to fungi can arise. The most common fungal postharvest diseases detected on Citrus limon var Eureka (Italian lime) produced in the Tamaulipas state are green/blue mold (Penicillium spp.), fusarium rot (F. oxysporum, F. solani, F. proliferatum, among others), and anthracnose (Colletotrichum spp.). In this work, we selected yeasts, occurring as the natural epiphytic mycoflora of lemons or from fermented traditional products, to be tested as part of a formulation for protecting stored lemons against fungal diseases. The best-performing yeasts, labeled as LCBG-03 (Meyerozyma guilliermondii), LCBG-30 (Pseudozyma sp.), and LCBG-49 (Saccharomyces cerevisiae), were selected to test their compatibility and biocontrol performance against strains of Penicillium digitatum (AL-38), Fusarium sp. (AL-21), Colletotrichum gloeosporioides (AL-13), and Epicoccum sorghinum (H3A). Based on their in vitro performance regarding the percentage of radial growth inhibition, both applied individually or as two yeasts mixed at equal cellular concentrations, the best combinations (containing M. guilliermondii formulated with either Pseudozyma sp. or S. cerevisiae) were selected with efficacies higher than 95% in both in vitro fungal radial growth rate inhibition and on stored lemon fruits. This work contributes to the search for compatible yeast combinations with the aim to diminish the fungal losses of citrus fruits using biocontrol for citrus postharvest protection.

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Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia

Nurlaelita,

Wibowo,

Widiastuti

2024

Plant Pathol J

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Dieback disease in mango trees has been observed in Indonesia, particularly in Java Island, with the causal agent remaining unidentified. One of the important pathogens that are responsible for causing mango dieback is Colletotrichum. Field surveys were conducted in various mango cultivating areas in Java Island, Indonesia to assess prevalence of Colletotrichum as dieback disease pathogen. Eleven Colletotrichum isolates were recovered from symptomatic dieback twigs and morphologically characterized. Genetic diversity fingerprint analysis was carried out using rep-PCR. Phylogenetic analysis identified isolates as belonging to Colletotrichum asianum and Colletotrichum cairnsense using partial sequences of four gene regions, including ITS, ACT, GAPDH, and TUB2. Pathogenicity tests on mango seedlings cv. Arumanis showed that all fungal isolates were responsible for causing dieback symptoms. Subsequently, symptomatic tissue was reisolated to fulfill Koch’s Postulate. This study represented new funding for two species of Colletotrichum causing mango dieback in Indonesia.

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Occurrence and infective potential of Colletotrichum gloeosporioides isolates associated to Citrus limon var Eureka

Cited by 3 publications

References 36 publications

Characterization of Colletotrichum species obtained from citrus in northern Thailand and introducing a new host record for C. plurivorum

Characterization of Colletotrichum species obtained from citrus in northern Thailand and introducing a new host record for C. plurivorum

Yeast Mixtures for Postharvest Biocontrol of Diverse Fungal Rots on Citrus limon var Eureka

Identification and Characterization Colletotrichum spp. Causing Mango Dieback in Indonesia

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