Occurrence and molecular characterisation of Vibrio parahaemolyticus in crustaceans commercialised in Venice area, Italy

Caburlotto, Greta; Suffredini, Elisabetta; Toson, M. A.; Fasolato, Luca; Antonetti, Paolo; Zambon, Michela; Manfrin, A.

doi:10.1016/j.ijfoodmicro.2015.12.007

Cited by 35 publications

(26 citation statements)

References 82 publications

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“…As is showed in Table 1, the most severely contaminated edible was shrimp (15.34%). However, the contamination detection rate was lower than that conveyed in other studies that report a prevalence of 28.0% (Caburlotto et al, 2016). The reason may be that the cities were sample collection was conducted were far away from the ocean, so these types of aquatic products were more scarce.…”

Section: Discussioncontrasting

confidence: 57%

“…Vibrio parahaemolyticus, a food-borne gastroenteritis-causing bacterium, is identified as a gram-negative bacterial cell that is common in seawater, seafood, and aquatic products. V. parahaemolyticus gastroenteritis outbreaks have been reported worldwide, including those in Bangladesh (Akther et al, 2016), Europe (Baker-Austin et al, 2010;Caburlotto et al, 2016;Lopatek et al, 2018), Japan (Arakawa et al, 1999), United States (Shaw et al, 2015) and South America (Raszl et al, 2016). In China, this bacterium is also a common causative agent of food poisoning (Xie et al, 2015;Dong et al, 2016) associated with consumption of fish, shellfish, and shrimp.…”

Section: Introductionmentioning

confidence: 99%

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Food-Borne Vibrio parahaemolyticus in China: Prevalence, Antibiotic Susceptibility, and Genetic Characterization

Xie

Pang

et al. 2020

Front. Microbiol.

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Vibrio parahaemolyticus is a marine and estuarine bacterium that leads to damage of aquatic industry by foodborne outbreaks and possesses an enormous threat to food safety as well as human health worldwide. In the current study, we investigated 905 food samples (ready-to-eat foods, fish, and shrimp) from 15 provinces in China, and aimed to determine prevalence, biological characteristics and genetic diversity of presumptive V. parahaemolyticus isolates. Firstly, 14.17% of 240 fish samples, 15.34% of 365 shrimp samples and 3.67% of 300 RTE food samples were positive for potential V. parahaemolyticus. Secondly, 69 food samples (14.87%) collected in summer were positive for target isolates, while the rate of positive sample of 441 food samples in winter reached 7.26%. Thirdly, we purified 202 V. parahaemolyticus strains for further research. And antimicrobial susceptibility results of strains tested revealed that the highest resistance rate was observed for ampicillin (79.20%). At the same time, 148 (73.27%) of all isolates were classified and defined as multi-drug resistant foodborne bacteria. The results of PCR assay showed that the isolates being positive for the tdh, trh or both genes, were up to 9.90%, 19.80% or 3.96%. Besides, multiplex PCR test showed that the isolates carrying O2 serogroup were the most prevalent. Furthermore, sequence types (STs) of 108 isolates were obtained via multi-locus sequence typing. Not only 82 STs were detected, but also 41 of which were updated in the MLST database. Thus, our findings significantly demonstrated the high contamination rates of V. parahaemolyticus in fish and shrimp and it may possess potential threat for consumer health. We also provided up-to-date dissemination of antibiotic-resistant V. parahaemolyticus which is important to ensure the high efficacy in the treatment of human and aquatic products infections. Lastly, with the identification of 82 STs including 41 novel STs, this study significantly revealed the high genetic diversity among V. parahaemolyticus. All of our research improved our understanding on microbiological risk assessment in ready-to-eat foods, fish, and shrimp.

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Section: Discussioncontrasting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Food-Borne Vibrio parahaemolyticus in China: Prevalence, Antibiotic Susceptibility, and Genetic Characterization

Xie

Pang

et al. 2020

Front. Microbiol.

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“…Total V. parahaemolyticus di tambak Tuban pada musim hujan berkisar 93-1100 MPN/g sedangkan jumlahnya pada musim kemarau relatif sama mencapai 1100 MPN/g. Pengaruh temperatur terhadap patogenisitas (Tabel 3) pada penelitian ini serupa dengan hasil penelitian Caburlotto et al (2016) yang melaporkan bahwa sampel positi f V. parahaemolyticus dari sampel udang yang berasal dari Laut Adriatik Utara dan Danau Venetia Utara, Italia terkonsentrasi di musim panas dan musim gugur (JuniNovember 2012), tetapi juga terdeteksi pada bulan Maret dan Desember 2012. Pada periode Juli hingga Desember di samping memiliki prevalensi yang tinggi juga memiliki nilai MPN tertinggi dengan peningkatan hingga bulan September, dan menurun secara tetap hingga akhir tahun.…”

Section: Karakterisasi Bakteri V Parahaemolyticusunclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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“…Namun demikian, insidensi V. parahaemolyticus yang bersifat patogen lebih cenderung terjadi pada musim kemarau (bulan Oktober) ketika suhu air tambak 31,69 °C dan pH 8,44 (Tabel 5). Dibanding bulan lainnya, suhu air tambak di bulan Oktober merupakan suhu yang paling mendekati kondisi optimum (37°C) untuk pertumbuhan bakteri V. parahaemolyticus (Lake, Hudson & Cressey, 2003 (Caburlotto et al, 2016). Sementara itu Lopez-Hernandez et al (2015) melaporkan bahwa dari 80 sampel kerang yang berasal dari Mandinga Grande Lagoon dan Mandinga City (Mandiga lagoon system) Veracruz, Meksiko ditemukan sebanyak 62,5% V. parahaemolyticus tdh dan 12,5% V. parahaemolyticus trh.…”

Section: Hasil Dan Bahasanunclassified

Keberadaan Vibrio parahaemolyticus Patogenik pada Udang Tambak yang Berasal dari Pantai Utara Jawa

Kusmarwati¹,

Hermana²,

Yennie³

et al. 2017

JPBKP

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Penelitian ini bertujuan untuk memberikan informasi cemaran Vibrio parahaemolyticus patogenik pada udang tambak. Pengambilan sampel udang segar vaname dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa dengan metode purposive random sampling. Sebanyak 103 sampel (masing-masing 33 sampel dari wilayah Jawa Barat, 14 sampel dari wilayah Jawa Tengah dan 56 sampel dari wilayah Jawa Timur) telah diestimasi kandungan V. parahaemolyticus total dan patogenik menggunakan metode kultivasi pada medium CHROMagarTM Vibrio (CV) dan metode polymerase chain reaction (PCR) melalui amplifikasi gen toxR untuk total V. parahaemolyticus, serta gen tdh dan trh untuk V. parahaemolyticus patogen. Hasil analisis menunjukkan bahwa dari 103 sampel, 91 sampel bersifat tipikal pada medium CHROM agarTM Vibrio (CV). Selanjutnya dari 91 sampel tipikal tersebut, 62 (63,27%) sampel menunjukkan hasil positif untuk V. parahaemolyticus dengan metode PCR. Sementara dari 31 sampel positif V. parahaemolyticus yang berasal dari Jawa Timur ditemukan 2 sampel (3,23%) positif mengandung gen tdh, 1 sampel (1,61%) positif mengandung gen tdh dan trh, dan 1 sampel (1,61%) positif mengandung gen trh.

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Occurrence and molecular characterisation of Vibrio parahaemolyticus in crustaceans commercialised in Venice area, Italy

Cited by 35 publications

References 82 publications

Food-Borne Vibrio parahaemolyticus in China: Prevalence, Antibiotic Susceptibility, and Genetic Characterization

Food-Borne Vibrio parahaemolyticus in China: Prevalence, Antibiotic Susceptibility, and Genetic Characterization

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Keberadaan Vibrio parahaemolyticus Patogenik pada Udang Tambak yang Berasal dari Pantai Utara Jawa

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