Occurrence and molecular characteristics of ESBL/AmpC-producing Escherichia coli in faecal samples from horses in an equine clinic

Apostolakos, Ilias; Franz, Eelco; Hoek, Angela H.A.M. van; Florijn, Alice; Veenman, Christiaan; Oldruitenborgh-Oosterbaan, Marianne M. Sloet-van; Dierikx, Cindy; Duijkeren, Engeline van

doi:10.1093/jac/dkx072

Cited by 41 publications

(70 citation statements)

References 29 publications

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“…Lastly, ST224, ST167, ST1308, and ST156 were detected in one isolate each. Notably, both ST10 and ST224 previously demonstrated their capacity for nosocomial infections and the spread of these sequence types between horses and potentially, to their human handlers [14,18,19]. In addition to being recovered from horses [14], ST224 was isolated from humans [11], swine [42], bovines [47], birds [44], and in this study, from a donkey.…”

Section: Discussionmentioning

confidence: 59%

“…ST410 was previously isolated from humans [38,39], canines, felines [40], swine, poultry, cattle [41][42][43], as well as birds [44]. E. coli ST10, identified in two of the 13 isolates, was recovered from humans, turkey meat, chickens, swine, cattle [45,46], and horses [19,20]. Lastly, ST224, ST167, ST1308, and ST156 were detected in one isolate each.…”

Section: Discussionmentioning

confidence: 99%

“…The results of the present study demonstrated that 27.08% of the isolates screened with ceftiofur expressed the ESBL-positive phenotype. Recent studies have reported the occurrence of ESBL-producing E. coli isolates recovered from equines to range from as low as 0.2% (one out of 508 isolates tested) in feral horses living on an isolated Canadian island [31], to 84% in equine patients at a veterinary teaching hospital in the Netherlands [19]. Additional studies detected ESBL-producing E. coli in 6.3% of fecal samples from equine patients across various veterinary practices in the United Kingdom [12], 10.1% of equine patients at a veterinary clinic in Germany [20], and 32% of equine patients at a veterinary clinic in the Czech Republic [3].…”

Section: Discussionmentioning

confidence: 99%

“…Of additional importance, the possibility of cross-species transmission of ESBL-positive bacterial strains directly represents a health hazard for humans, especially equine handlers and veterinary staff [15,17,18]. Despite this significant problem, however, information on the occurrence and genetic characterization of ESBL-positive E. coli isolated from horses is lacking [3,19,20]. Of the studies that have investigated ESBL-positive E. coli in equines, the isolates were predominantly originated from fecal samples.…”

mentioning

confidence: 99%

“…Of the studies that have investigated ESBL-positive E. coli in equines, the isolates were predominantly originated from fecal samples. To date, very few studies have thoroughly evaluated the occurrence of ESBL-positive E. coli in diagnostic specimens (other than feces) from equine patients [14,19]. As such, the objective of the present work was to examine the proportion and genetic diversity of ESBL-positive E. coli in clinical specimens of equine origin.…”

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confidence: 99%

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The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

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Simple Summary: The spread and development of extended spectrum beta-lactamase (ESBL)-mediated antimicrobial resistance is a significant concern in healthcare with impacts to animal and public health alike. While the occurrence of the ESBL phenotype in Escherichia coli has been investigated in depth by numerous studies, there is still a lack of information regarding ESBL-producing bacterial isolates from clinical specimens of equine origin. In this study, we investigated the incidence of ESBL-producing E. coli in hospitalized horses. Overall, 207 E. coli isolates were analyzed for their antimicrobial susceptibility and 13 ESBL-producing E. coli isolates were genotypically characterized. Seven out of the 13 E. coli isolates were found to harbor the resistance genes bla CTX-M-1 or bla SHV-1 and a novel beta-lactamase TEM gene variant, bla TEM-233 was discovered. Furthermore, despite being phenotypically susceptible to tested carbapenems, 1 out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 . The latter is an alarming finding because the presence of carbapenemase resistance genes in equine pathogens is extremely rare. In conclusion, equines can be reservoirs for ESBL-producing Enterobacteriaceae, and further investigation into this species group is necessary to understand their impact in the spread and development of antibiotic resistance genes.Abstract: Escherichia coli isolates were recovered from clinical specimens of equine patients admitted to the Texas A&M Veterinary Medical Teaching Hospital over a five-year period. Ceftiofur resistance was used as a marker for potential extended-spectrum beta-lactamase (ESBL)-activity, and of the 48 ceftiofur-resistant E. coli isolates, 27.08% (n = 13) were phenotypically ESBL-positive. Conventional PCR analysis followed by the large-scale bla Finder multiplex PCR detected the ESBL genes, CTX-M-1 and SHV, in seven out of the 13 isolates. Moreover, beta-lactamase genes of TEM-1-type, BER-type (AmpC), and OXA-type were also identified. Sequencing of these genes resulted in identification of a novel TEM-1-type gene, called bla TEM-233 , and a study is currently underway to determine if this gene confers the ESBL phenotype. Furthermore, this report is the first to have found E. coli ST1308 in horses. This subtype, which has been reported in other herbivores, harbored the SHV-type ESBL gene. Finally, one out of 13 E. coli isolates was PCR-positive for the carbapenemase gene, bla IMP-1 despite the lack of phenotypically proven resistance to imipenem. With the identification of novel ESBL gene variant and the demonstrated expansion of E. coli sequence types in equine patients, this study underscores the need for more investigation of equines as reservoirs for ESBL-producing pathogens. ]. The wide application of beta-lactam antibiotics has been considered as a driving factor in the development and spread of extended-spectrum beta-lactamase (ESBL)-conferred resistance in Gram-negative bacterial pathogens such as Escherichia coli, Klebsiella spp., and Sa...

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Elias

Gillis

Gurrola-Rodriguez

et al. 2019

Animals

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Does colonization with MRSA, ESBL – producingEnterobacteriaceae, and/orAcinetobacter baumannii– increase the risk for postoperative surgical site infection?

Gehlen

Klein

Merle

et al. 2023

Veterinary Medicine & Sci

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Objective: Evaluation of the role of indicator pathogens in equine surgical site infection (SSI) and other infection-promoting factors. Study design:Cross-sectional study. Animals:Horses presenting with an open injury or surgical colic during 1.5 years. Methods:A nasal swab and a faecal sample were collected from every patient upon admission. Furthermore, a wound swab was collected from wounds of injured horses.Details on the wounds and procedures were documented. Laparotomy incisions and injuries were monitored for signs suggesting infection. Results:In total, 156 horses presented because of a surgical colic (n = 48) or open injuries (n = 108). Thirteen surgical colic patients and three injured horses did not survive beyond 24 h, and four injured horses were discharged from the clinic at the day of admission. SSIs occurred in 31 (30.7%) injured horses and 11 (31.4%) horses after laparotomy. Regarding injuries, general anaesthesia increased the risk of developing a WI compared to sedation. Indicator pathogens were cultured from 29/42 SSI.In total, 10/11 infected laparotomy incisions and 19/31 injuries with SSI tested positive for multidrug-resistant pathogens (MDRPs) . Indicator pathogens were not detected at admission in any of the horses that developed incisional SSIs after laparotomy but were detected in two of the injured horses that developed SSIs. Conclusion:MDRPs were identified in almost 70% of the SSI. Less than 5% of the affected animals were colonized with the same pathogen before admission, indicating that colonization with MDR pathogens is only one of the crucial factors for the development of SSI.

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Assessing antimicrobial and metal resistance genes in Escherichia coli from domestic groundwater supplies in rural Ireland

Andrade

Ryan²,

Burke

et al. 2023

Environmental Pollution

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Occurrence and molecular characteristics of ESBL/AmpC-producing Escherichia coli in faecal samples from horses in an equine clinic

Cited by 41 publications

References 29 publications

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

The Occurrence and Characterization of Extended-Spectrum-Beta-Lactamase-Producing Escherichia coli Isolated from Clinical Diagnostic Specimens of Equine Origin

Does colonization with MRSA, ESBL – producingEnterobacteriaceae, and/orAcinetobacter baumannii– increase the risk for postoperative surgical site infection?

Assessing antimicrobial and metal resistance genes in Escherichia coli from domestic groundwater supplies in rural Ireland

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