Occurrence and molecular identification of Vibrio parahaemolyticus in retail shellfish in Mansoura, Egypt

Detection and Molecular Characterization of Vibrio Parahaemolyticus in Shrimp Samples

“…The V. parahaemolyticus is an enteric human pathogen that occurs naturally in the marine and estuarine environments worldwide. Several outbreaks of seafood poisoning were caused by V. parahaemolyticus in many countries and regions of the world including USA, Japan, India and Taiwan [10]. In this study, a total of 70 individual wild shrimp samples were studied for the presence of pathogenic V. parahaemolyticus.…”

“…Confirmation of presumptive V. parahaemolyticus isolates was performed using PCR targeting the vp-toxR gene with the following primers and amplicon size 368bp: toxR-F:5'-GTCTTCTGACGCAATCGTTG-3' and toxR-R:5'-ATACGAGTGGTTGCTGTCATG-3' [2]. A specific primer pairs were also used for detection of tdh and trh virulence genes in toxR positive strains with the following sequences: tdh-F:5'-CCACTACCACTCTCATATGC-3' , tdh-R:5'-GGTACTAAATGGCTGACATC-3' with amplicon size 251 bp and trh-F:5'GGCTCAAAATGGTTAAGCG -3' and trh-R:5'-CATTTCCGCTCTCATATGC-3' with amplicon size 250 bp [10]. Single PCR was performed using Dream Taq PCR Master Mix (Thermo Fisher Scientific), which contains Taq polymerase, dNTPs, MgCl 2 and the appropriate buffer.…”

“…There are different methods for detection of V. parahaemolyticus in seafood samples. The most-probable-number (MPN) method is used for enumeration of V. parahaemolyticus from food and water, but this method is cumbersome and the recovery of the organism is low [15].The culture-based approaches and PCR technique which is faster, easier and more sensitive can be used for identification of V. parahaemolyticus in seafood samples [10]. The V. parahaemolyticus strains possess a regulatory gene, toxR, which is present in all strains irrespective of their ability to produce tdh and/or trh.Therefore, the PCR targeted to the toxR gene can be used as a method for identification at the species level [16].…”

Prevalence of Vibrio parahaemolyticus in Penaeus Monodon (Fabricius, 1798) from the Douala Coastal Waters of Cameroon: Implication for Food Safety

“…It is a facultative anaerobic organism that naturally inhabits estuarine and coastal waters worldwide [4,5]. It has been isolated from sediment, suspended particles [6] and from a wide variety of marine organisms [7], such as Crustaceans [8][9][10] and Molluscs [11].…”

“…Vibrio parahaemolyticus has been identified in a variety of seafood including shrimps, crabs, oysters and clams due to its halophilic characteristic and seawater habitat throughout the world including in Italy [4,15,]; in Germany [16], in USA [12,5], in India [17][18][19][20][21][22], in Iran [23][24][25], in Malaysia [26][27][28][29]11], in Egypt [30,10], in Nigeria [8,[31][32], in Cameroon, from fresh water prawn [33]. Vibrio parahaemolyticus has been reported from cultured shrimps, hatcheries and aquaculture ponds [34][35][36][37].…”