Occurrence and new procedure of preparation of nigrin, an antiribosomal lectin present in elderberry bark

Ferreras, José M.; Citores, Lucı́a; Iglesias, Rosario; Jiménez, Pilar; Souza, Aíla Maria; Gayoso, Manuel J.; Girbés, Tomás

doi:10.1016/j.foodres.2011.06.004

Cited by 13 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Figure 2, with the exception of SNA-I, which contained a small amount of nigrin b, they were essentially pure as judged by SDS-PAGE. The present results indicate that the modified protocol for nigrin b isolation heralds an improvement on previously reported procedures which used frozen elderberry bark [29,40].…”

Section: Nigrin B Isolationsupporting

confidence: 51%

Plasma Accumulations of Vitamin B6 from an Oral Dose in a New Reversible Model for Mouse Gut Injury and Regeneration

Jiménez¹,

Córdoba-Díaz²,

Cabrero³

et al. 2013

FNS

Self Cite

View full text Add to dashboard Cite

Chemically based rodent models are used to assess the positive effects promoted by foods and gut microbiota on gut health. Lectins with enzymatic activity, such as type 2 ribosome-inactivating proteins, might also prove useful for exploring these issues. Sub-lethal doses of the lectin nigrin from Sambucus nigra L. to mice promoted reversible derangement of gut epithelium by induction of apoptosis of transit amplifying cells of the small intestine crypts in a time-dependent course. The present work seeks to study vitamin B6 accumulation in plasma from an oral bolus in a mouse nigrin model. 24 h after sub-lethal nigrin b treatment, there was clear body weight reduction associated to a notable increase in Evan’s blue stain accumulation in excised small intestine, an increase in myeloperoxidase activity, and a near 50% reduction in plasma accumulation of vitamin B6. Histological analysis of small intestine sections of nigrin b-treated animals also revealed significant derangement of intestinal crypts. Seventy two hours after nigrin b treatment, stain uptake decreased and vitamin B6 accumulation was almost restored despite villi derangement. Large intestine crypts were scarcely or not at all affected. Eight days after nigrin b treatment, vitamin B6 uptake and intestinal crypt structure had fully recovered. The nigrin b mice model supports the view that, under these conditions, the carrier-mediated vitamin B6 uptake component of the small intestine crypts is probably the most active when the vitamin is administered orally as a bolus. The findings provide insights into the suitability of the present mice model for nutritional or drug absorption studies in conditions of partially altered or injured intestinal mucosa.

show abstract

Section: Nigrin B Isolationsupporting

confidence: 51%

Plasma Accumulations of Vitamin B6 from an Oral Dose in a New Reversible Model for Mouse Gut Injury and Regeneration

Jiménez¹,

Córdoba-Díaz²,

Cabrero³

et al. 2013

FNS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Therefore, these dimeric lectins appear to be unique to the leaves. It is also noteworthy that in leaves, no tetrameric type 2 RIPs corresponding to SNAI from the bark, SNAIf from the fruits, or SNAflu-I from flowers were found, tissues in which this type of structure is among the predominant [13,51,[55][56][57].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we aimed to isolate and characterize the RIPs and lectins from elderberry leaves. For this purpose, we optimized a standard RIP purification procedure [12] previously used to isolate RIPs and lectins from elderberry bark [13] and dwarf elder leaves [14]. A schematic overview of the procedures used to purify the RIPs and lectins from S. nigra leaves is shown in Figure 1.…”

Section: Isolation Of Rips and Lectins From Elderberry Leavesmentioning

confidence: 99%

Structure and Biological Properties of Ribosome-Inactivating Proteins and Lectins from Elder (Sambucus nigra L.) Leaves

Iglesias

Russo

Valletta

et al. 2022

Toxins

Self Cite

View full text Add to dashboard Cite

Ribosome-inactivating proteins (RIPs) are a group of proteins with rRNA N-glycosylase activity that catalyze the removal of a specific adenine located in the sarcin–ricin loop of the large ribosomal RNA, which leads to the irreversible inhibition of protein synthesis and, consequently, cell death. The case of elderberry (Sambucus nigra L.) is unique, since more than 20 RIPs and related lectins have been isolated and characterized from the flowers, seeds, fruits, and bark of this plant. However, these kinds of proteins have never been isolated from elderberry leaves. In this work, we have purified RIPs and lectins from the leaves of this shrub, studying their main physicochemical characteristics, sequences, and biological properties. In elderberry leaves, we found one type 2 RIP and two related lectins that are specific for galactose, four type 2 RIPs that fail to agglutinate erythrocytes, and one type 1 RIP. Several of these proteins are homologous to others found elsewhere in the plant. The diversity of RIPs and lectins in the different elderberry tissues, and the different biological activities of these proteins, which have a high degree of homology with each other, constitute an excellent source of proteins that are of great interest in diagnostics, experimental therapy, and agriculture.

show abstract

“…Interest in RIPs derives from their toxicity and from their possible use in anticancer immunotoxins and conjugates [30,[32][33][34]. Some of these RIPs show lectin properties, being known as ribosome-inactivating lectins (RILs), such as nigrin b from elderberry bark [35] and ebulin f from dwarf elder fruits [36,37]. Both nigrin b and ebulin f display dose-dependent toxicities depending on the different administration way [35,[37][38][39].…”

Section: Introductionmentioning

confidence: 99%

“…Some of these RIPs show lectin properties, being known as ribosome-inactivating lectins (RILs), such as nigrin b from elderberry bark [35] and ebulin f from dwarf elder fruits [36,37]. Both nigrin b and ebulin f display dose-dependent toxicities depending on the different administration way [35,[37][38][39]. Both nigrin b and ebulin f administration promoted apoptosis of intestinal crypt cells in mice, as revealed by histological analysis [38][39][40].…”

Section: Introductionmentioning

confidence: 99%

Unexpected Toxicity of Green Tea Polyphenols in Combination with the Sambucus RIL Ebulin

Garrosa

Jiménez

et al. 2020

Toxins

Self Cite

View full text Add to dashboard Cite

The safety of concentrated food complements intake is a major health concern. It has been well established that green tea polyphenols (GTPs) consumption promotes healthy effects. However, the ingestion of large amounts of GTPs is a matter of controversy due to reported adverse effects. We underwent a preliminary exploration of the effects of the oral administration of a standardized concentrated GTPs preparation on mice which suffered from reversible intestinal derangement promoted by sublethal amounts of the antiribosomal lectin ebulin f from dwarf elder (Sambucus ebulus L.). Neither independent oral administration of 30 mg/kg body weight Polyphenon 60 nor intraperitoneal administration of 2.5 mg/kg body weight ebulin f triggered lethal toxicity. In contrast, the simultaneous administration of these same doses of both Polyphenon 60 and ebulin f triggered an important and unexpected synergistic toxic action featured by the biphasic reduction of weight, which continued after eight days, reaching a reduction of 40%. Lethality appeared 2 days after the onset of the combined treatment and reached more than 50% after 10 days.

show abstract

Occurrence and new procedure of preparation of nigrin, an antiribosomal lectin present in elderberry bark

Cited by 13 publications

References 47 publications

Plasma Accumulations of Vitamin B6 from an Oral Dose in a New Reversible Model for Mouse Gut Injury and Regeneration

Plasma Accumulations of Vitamin B6 from an Oral Dose in a New Reversible Model for Mouse Gut Injury and Regeneration

Structure and Biological Properties of Ribosome-Inactivating Proteins and Lectins from Elder (Sambucus nigra L.) Leaves

Unexpected Toxicity of Green Tea Polyphenols in Combination with the Sambucus RIL Ebulin

Contact Info

Product

Resources

About