Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

Sethulekshmi, C; Latha, C.; Anu, C. J.

doi:10.14202/vetworld.2018.104-111

Cited by 10 publications

(5 citation statements)

References 32 publications

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“…our results demonstrate that the percentage of E. coli isolated from meat and butcher shops was 27.4% (138/504). In addition, this study showed the percentage of E. coli was rising in meat at 41.7% (35/84), which agreed with the previous surveys that found the percentage of E. coli in meat was 43.1% in India (23), 43.4 % in the United States (24). However, our results are lower than the previous surveys that appeared the percentage of E. coli from meat in Egypt was 54% (27/50) (25), 74.5% in South Africa (26), and 100% in Burkina Faso (27).…”

Section: Discussionsupporting

confidence: 92%

Phenotypic and genotypic characterizations of Escherichia coli Isolated from veal meats and butchers’ shops in Mosul city, Iraq

Othman¹,

Sheet²,

Al-Sanjary³

2023

IJVS

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Foodborne pathogens bacteria can cause various diseases and death worldwide. Escherichia coli is the most crucial microorganism transmitted through meat and its products. Pathogenic E. coli is one of the major groups that can produce the Stx1 and Stx2 toxins. The present study aims to isolate and identify the E. coli bacteria using the classical methods, and to detect the specific-species uidA gene, and Stx1 and Stx2 genes using the PCR assay. Five hundred four samples were collected randomly from meats and different parts of a butcher shops from various regions of the right and the left sides of Mosul city. The results found that the prevalence rate of E. coli in this study was 27.4% (138/504). Additionally, the prevalence rate of E. coli was higher in meat, 41.7% (35/84). At the same time, the prevalence rate of E. coli was lower in hook 16.7% (14/84). Additionally, the prevalence rate of E. coli in meats and butcher shops on the right and left sides of Mosul city was 31.9% (65/204) and 24.3% (73/300), respectively. Furthermore, all E. coli isolates possessed the specific species uidA gene. 30/138 (21.7%) of E. coli isolates possess the Stx1 gene, while 17/138 (12.3%) of E. coli isolates have the Stx2 gene. Finally, most E. coli isolates possessed the Stx1 and Stx2 genes 91/138 (66%).

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Section: Discussionsupporting

confidence: 92%

Phenotypic and genotypic characterizations of Escherichia coli Isolated from veal meats and butchers’ shops in Mosul city, Iraq

Othman¹,

Sheet²,

Al-Sanjary³

2023

IJVS

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“…In Iran, a study was conducted by Mohammadi et al (2013) who found that the prevalence of STEC in raw milk was 17.47 % (36 out of 206 samples). More or less similar results were reported in previous studies by Abadu et al (2020) andSethulekshmi et al (2018) who found that the prevalence of STEC in raw milk samples were, 19% and 19.26%, respectively. Moreover, similar results were recorded in Germany byZschock et al, (2000) who stated that STEC in raw milk was 18%.…”

supporting

confidence: 91%

Prevalence and Antibiogram profiles of STEC strains isolated from raw cow's milk in New-Valley Governorate, Egypt

Mohamed

Hassan

Ezzat

et al. 2022

SVU-International Journal of Veterinary Sciences

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A cross sectional study was conducted to isolate and identify Shiga toxinproducing Escherichia coli (STEC) from raw cow milk. A total of 267 milk samples were collected from apparently healthy cattle in 7 different dairy farms. Thirty-nine STEC isolate were identified out of 86-positive E. coli strains (56.98%). The prevalence of E. coli and STEC was 32.2% and 18.4%, respectively. Antimicrobial susceptibility of the isolated STEC was determined by disc diffusion method. A total of 16 antimicrobials of seven antimicrobial classes were used. Commercially available antibiotic disc namely aminoglycosides [Erythromycin (15 μg), Clindamycin (2μg, Kanamycin (30 μg, Amikacin (30 μg) , Gentamicin (10 μg), cephalosporins (Cefepime (30 μg, Cefotaxime (30μg), Cefazolin (30μg], Fluoroquinolones [Naladixic acid (30 μg), Ciprofloxacin (5 μg), Levofloxacin (5μg)], penicillin (Ampicillin 10 μg),Tetracycline (tetracycline 30 μg), Sulphonamides (Sulphamethoxazol 30 μg, and Carbapenems [Meropenem 10 μg, Imipenem 10 μg] were used. E. coli strain ATCC25922 was used as a control strain in the experiment. Highest resistance was observed against erythromycin (100%) followed by cefepime (97.4%) and clindamycin (82.1%), Nalidixic acid (61.5%), sulphamethazon (48.7%), tetracycline (41.0), kanamycin (33.3%), and cefotaxime (33.3%). Carapenems were found to be the most effective antimicrobial group where the examined STEC isolates were susceptible to Imipinem at 97.4% (38/39), followed by Meropenem at 94.9% (37/39). Results showed that multidrug resistance STEC isolates were 81.25% of the tested antimicrobial (13/16). In conclusion, high prevalence of STEC in raw milk indicate insufficient hygienic measures adopted during milking and handling and verify that raw milk is potential source of these multidrug resistant strains.

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“…STEC causes many infections in humans, including gastrointestinal illnesses including non-bloody or bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome [ 53 ], which has been infrequently identified in camels. The transmission of STEC usually occurs through contaminated foods, water, and person-to-person spread [ 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt

et al. 2021

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This study aims to determine the prevalence of STEC in she-camels suffering from mastitis in semi-arid regions by using traditional culture methods and then confirming it with Serological and molecular techniques in milk samples, camel feces, as well as human stool samples for human contacts. In addition, an antibiotic susceptibility profile for these isolates was investigation. Mastitic milk samples were taken after California Mastitis Test (CMT) procedure, and fecal samples were taken from she-camels and human stool samples, then cultured using traditional methods to isolate Escherichiacoli. These isolates were initially classified serologically, then an mPCR (Multiplex PCR) was used to determine virulence genes. Finally, both camel and human isolates were tested for antibiotic susceptibility. Out of a total of 180 she-camels, 34 (18.9%) were mastitic (8.3% clinical and 10.6% sub-clinical mastitis), where it was higher in camels bred with other animals. The total presence of E. coli was 21.9, 13.9, and 33.7% in milk, camel feces, and human stool, respectively, whereas the occurrence of STEC from the total E. coli isolates were 36, 16, and 31.4% for milk, camel feces, and stool, respectively. Among the camel isolates, stx1 was the most frequently detected virulence gene, while hlyA was not detected. The most detected virulence gene in human isolates was stx2 (45.5%), followed by stx1. Camel STEC showed resistance to Oxytetracycline only, while human STEC showed multiple drug resistance to Amoxicillin, Gentamycin, and Clindamycin with 81.8, 72.7, and 63.6%, respectively. Breeding camels in semi-arid areas separately from other animals may reduce the risk of infection with some bacteria, including E. coli; in contrast, mixed breeding with other animals contributes a significant risk factor for STEC emergence in camels.

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Occurrence and quantification of Shiga toxin-producing Escherichia coli from food matrices

Cited by 10 publications

References 32 publications

Phenotypic and genotypic characterizations of Escherichia coli Isolated from veal meats and butchers’ shops in Mosul city, Iraq

Phenotypic and genotypic characterizations of Escherichia coli Isolated from veal meats and butchers’ shops in Mosul city, Iraq

Prevalence and Antibiogram profiles of STEC strains isolated from raw cow's milk in New-Valley Governorate, Egypt

Molecular Detection, Serotyping, and Antibiotic Resistance of Shiga Toxigenic Escherichia coli Isolated from She-Camels and In-Contact Humans in Egypt

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