Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France

Azandegbé, Afi; Garnier, Matthieu; Andrieux-Loyer, Françoise; Kérouel, Roger; Philippon, Xavier

doi:10.3354/dao02253

Cited by 26 publications

(28 citation statements)

References 38 publications

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“…The most significant result was the influence of seasonality on species diversity. This is consistent with previous work conducted both on the Vibrio genus and on single species in different parts of the world (48,50,59). We performed several statistical and population analyses to understand whether ecological variables could be linked to particular species and which of them were the most informative.…”

Section: Discussionsupporting

confidence: 82%

Vibrio Trends in the Ecology of the Venice Lagoon

Rahman

Martino

Cardazzo

et al. 2014

Appl Environ Microbiol

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Vibrio is a very diverse genus that is responsible for different human and animal diseases. The accurate identification of Vibrio at the species level is important to assess the risks related to public health and diseases caused by aquatic organisms. The ecology of Vibrio spp., together with their genetic background, represents an important key for species discrimination and evolution. Thus, analyses of population structure and ecology association are necessary for reliable characterization of bacteria and to investigate whether bacterial species are going through adaptation processes. In this study, a population of Vibrionaceae was isolated from shellfish of the Venice lagoon and analyzed in depth to study its structure and distribution in the environment. A multilocus sequence analysis (MLSA) was developed on the basis of four housekeeping genes. Both molecular and biochemical approaches were used for species characterization, and the results were compared to assess the consistency of the two methods. In addition, strain ecology and the association between genetic information and environment were investigated through statistical models. The phylogenetic and population analyses achieved good species clustering, while biochemical identification was demonstrated to be imprecise. In addition, this study provided a fine-scale overview of the distribution of Vibrio spp. in the Venice lagoon, and the results highlighted a preferential association of the species toward specific ecological variables. These findings support the use of MLSA for taxonomic studies and demonstrate the need to consider environmental information to obtain broader and more accurate bacterial characterization. Vibrio spp. are Gram-negative halophilic bacteria belonging to the class Gammaproteobacteria. Vibrio is one of the most studied and diverse genera of microorganisms found in aquatic ecosystems and comprises the major culturable bacteria in marine and estuarine environments (1). According to the Association of Vibrio Biologists (AViB; http://www2.ioc.fiocruz.br/vibrio/AVib /species.html), there are 99 accepted or proposed Vibrio species, although the recent description of new species has led to a constantly changing taxonomy. Vibrio spp. are frequently isolated from fish, fish products, and edible shellfish, and a large number of species are pathogenic to different hosts. Some species, such as V. cholerae, V. parahaemolyticus, and V. vulnificus, cause serious food-borne gastroenteritis in humans. Other species, such as V. anguillarum and V. salmonicida, are pathogenic for fish; V. splendidus-related species are pathogenic for bivalves, and V. harveyi and V. campbellii are pathogenic for shrimps (1, 2, 3). Recently, Austin suggested a classification of zoonotic Vibrio in two classes named "higher-risk" vibrios (V. cholerae, V. parahaemolyticus, and V. vulnificus) and "lower-risk" vibrios (V. alginolyticus, V. fluvialis, V. furnissii, V. harveyi, and V. mimicus) (4). Bivalve mollusks such as clams and mussels represent products of great econ...

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Section: Discussionsupporting

confidence: 82%

Vibrio Trends in the Ecology of the Venice Lagoon

Rahman

Martino

Cardazzo

et al. 2014

Appl Environ Microbiol

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“…abundance in the aquatic environment, it was reaffirmed that temperature is a strong predictor of abundance and distribution of total vibrios (3,4,30,31,33,42,52,54,63,74,77), and this is particularly useful in the warmer Gulf Coast states included in this study (LA and MS). Though it is clear that temperature is dominant, there is no specific hierarchy among the parameters; environmental factors interact to influence vibrio abundance, but precise details of all such variables and how they interact have yet to be fully described.…”

Section: Discussionmentioning

confidence: 99%

Ecology of Vibrio parahaemolyticus and Vibrio vulnificus in the Coastal and Estuarine Waters of Louisiana, Maryland, Mississippi, and Washington (United States)

Johnson

Bowers

Griffitt

et al. 2012

Appl Environ Microbiol

191

183

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f Vibrio parahaemolyticus and Vibrio vulnificus, which are native to estuaries globally, are agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous to coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea surface height (SSH), and chlorophyll have been shown to be predictors of zooplankton and thus factors linked to vibrio populations. The contribution of salinity, conductivity, turbidity, and dissolved organic carbon to the incidence and distribution of Vibrio spp. has also been reported. Here, a multicoastal, 21-month study was conducted to determine relationships between environmental parameters and V. parahaemolyticus and V. vulnificus populations in water, oysters, and sediment in three coastal areas of the United States. Because ecologically unique sites were included in the study, it was possible to analyze individual parameters over wide ranges. Molecular methods were used to detect genes for thermolabile hemolysin (tlh), thermostable direct hemolysin (tdh), and tdh-related hemolysin (trh) as indicators of V. parahaemolyticus and the hemolysin gene vvhA for V. vulnificus. SST and suspended particulate matter were found to be strong predictors of total and potentially pathogenic V. parahaemolyticus and V. vulnificus. Other predictors included chlorophyll a, salinity, and dissolved organic carbon. For the ecologically unique sites included in the study, SST was confirmed as an effective predictor of annual variation in vibrio abundance, with other parameters explaining a portion of the variation not attributable to SST.

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“…V. splendidus and related species were also detected in Chamelea gallina (Torresi et al 2011). Other Vibrio species, such as V. aestuarianus, cause mortalities in Pacific oyster (Garnier et al 2008, Azandégbé et al 2010, Labreuche et al 2010, and V. harveyi has also been associated with mass mortality episodes in the abalone Haliotis tuberculata .…”

Section: Introductionmentioning

confidence: 99%

Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis

Romero

Costa²,

Forn-Cuní³

et al. 2014

Dis. Aquat. Org.

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Widespread and large-scale mortalities of bivalve molluscs significantly affect their production. A number of pathogens have been identified as the primary causes of death in oysters or clams, especially bacteria of the genus Vibrio. We evaluated the occurrence, seasonality and infectivity of Vibrio strains associated with natural mussel (Mytilus galloprovincialis) populations. In particular, different isolates of V. splendidus and V. aestuarianus were analysed because they were associated with major oyster mortalities in areas where mussels are cultured without presenting mortalities. The presence of both Vibrio spp. was analysed bimonthly in mussels, water, sediment, plankton and other associated fauna from 2 sites in Galicia (NW Spain), the region with the highest mussel production in Europe. Environmental factors were also considered. The pathogenicity of different Vibrio isolates was analysed by performing experimental infections in mussels with strains isolated from the field. Results showed that Vibrio populations were mainly influenced by changes in water temperature and salinity. V. splendidus was dominant during the warm months and V. aestuarianus was predominant throughout the cold season. The sediment was the most important natural reservoir for bacteria. Experimental infections showed the extreme resistance of mussels to bacterial pathogens. Isolates of V. splendidus and V. aestuarianus were only moderately pathogenic for mussels in intramuscular infections and bath infections, and mortalities only occurred when animals were infected with a high bacterial concentration in adverse environmental conditions (hypoxia and 25°C). Although the pathogenicity of the Vibrio strains isolated from the wild was low for mussels, their potential risk for other bivalves cannot be ignored.

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Occurrence and seasonality of Vibrio aestuarianus in sediment and Crassostrea gigas haemolymph at two oyster farms in France

Cited by 26 publications

References 38 publications

Vibrio Trends in the Ecology of the Venice Lagoon

Vibrio Trends in the Ecology of the Venice Lagoon

Ecology of Vibrio parahaemolyticus and Vibrio vulnificus in the Coastal and Estuarine Waters of Louisiana, Maryland, Mississippi, and Washington (United States)

Occurrence, seasonality and infectivity of Vibrio strains in natural populations of mussels Mytilus galloprovincialis

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