Occurrence and specificity of IgE antibodies to isocyanates in occupationally exposed workers

Cvitanović, Slavica; Zekan, L; Marušić, Matko

doi:10.1007/bf00386483

Cited by 9 publications

(5 citation statements)

References 21 publications

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“…Tee et al (1998) also reported that subtracting HSA binding from conjugate binding improved test performance in discriminating among OA cases and noncases. For classification of positive responses, a RAST ratio of 2 or higher has been used most often (Keskinen et al, 1988;Cvitanovic et al, 1989;Park and Nahm, 1996, Deschamps et al, 1998, Nosko et al, 1998, but a ratio of 3+ (Wass and Belin, 1989) has also been employed, as well as a RAST ratio greater than the highest of 22 nonexposed controls (Welinder et al, 1988). Few studies have examined the consequences of using different definitions for a positive response on test performance.…”

Section: Defining a Positive Test Resultsmentioning

confidence: 97%

Issues in Diisocyanate Antibody Testing

Jolly

Burkert

et al. 2007

Critical Reviews in Toxicology

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Diisocyanates are used to produce a wide variety of polyurethane products; they are also recognized as an important cause of occupational asthma. Their chemical reactivity presents challenges to toxicologists and clinicians alike seeking to understand the mechanisms underlying diisocyanate asthma. In this article, we review the literature on immunoassay detection of IgE and IgG binding to diisocyanate-protein conjugates and assess the utility of such testing as a diagnostic tool and exposure indicator. Data from 29 studies of occupational exposure to diisocyanates revealed considerable variability in assay methodology and heterogeneity in the prevalence of positive antibody responses across laboratories. In studies that included both confirmed diisocyanate asthma subjects and exposed nonasthmatics, positive IgE responses identified cases with low sensitivity (18-27%), but high specificity (96-98%). Detection of IgG binding to diisocyanate conjugates is an indirect, qualitative indicator of disease status and past diisocyanate exposure. The utility of these assays is limited, however, due to a lack of (1) method standardization, (2) population norms to guide interpretation of results, and (3) demonstration that the assays improve either on disease prediction or on exposure confirmation beyond that of other indicators. Sources of assay heterogeneity are discussed and suggestions are offered for improving test performance and interpretability.

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Section: Defining a Positive Test Resultsmentioning

confidence: 97%

Issues in Diisocyanate Antibody Testing

Jolly

Burkert

et al. 2007

Critical Reviews in Toxicology

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“…The argument that immunologic mechanisms lie behind isocyanate-induced bronchoconstriction is supported by the detection of serum-specific IgE [2, 4, 6, 7, 8]and IgG [2, 9]antibodies to isocyanates. However, the significance of specific IgE and IgG antibodies in the pathogenesis of isocyanate-induced asthma remains unclear, since most patients have no evidences of antibodies to isocyanates [2, 4, 6, 7, 8, 9].…”

Section: Introductionmentioning

confidence: 90%

“…However, the significance of specific IgE and IgG antibodies in the pathogenesis of isocyanate-induced asthma remains unclear, since most patients have no evidences of antibodies to isocyanates [2, 4, 6, 7, 8, 9]. …”

Section: Introductionmentioning

confidence: 99%

Hexamethylene Diisocyanate Causes Contraction of Canine Tracheal Smooth Muscles through Activation of Muscarinic Receptors

Tomari¹,

Shimoda²,

Kawano³

et al. 2000

Int Arch Allergy Immunol

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Background: Asthma caused by occupational exposure to hexamethylene diisocyanate (HDI) is well known; however, the exact pathogenic mechanisms remain unclear. Methods: Experiments were performed using a standard canine tracheal smooth muscle (CTSM) strip preparation in an isolated bath to determine the effect of HDI on tracheal smooth muscle contraction. HDI concentration-response curves were constructed and the effects of different receptor antagonists on HDI-induced smooth muscle contraction were determined. To determine whether HDI and acetylcholine (ACh) bind to a common muscarinic receptor, ACh concentration-response curves in the absence or presence of HDI and concentration-response curves for HDI and ACh in the presence or absence of atropine were plotted. Results: HDI caused contraction of CTSM, with a threshold concentration of 10^–7 M. The EC₅₀ (HDI concentration that produced 50% of the maximal response) was 6.2 ± 0.7 × 10^–7 M and the maximal contractile response (174 ± 55 g/g of tissue) occurred at a concentration of 5.0 ± 0.8 × 10^–6 M. Atropine, a muscarinic blocker, significantly inhibited HDI-induced contractile responses. HDI shifted the ACh concentration-response curve to the right. The mean pA₂ for atropine against ACh (8.93 ± 0.27) was not significantly different from that against HDI (8.03 ± 0.12). Conclusions: Our results indicated that HDI causes contraction of CTSM through the activation of muscarinic receptors. Direct stimulation of muscarinic receptors by HDI may play an important role in HDI-induced asthma.

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“…IgE antibodies are classically associated with immediate hypersensitivity reactions and are associated with acute exposures to isocyanates and changes in pulmonary function. 9,10 IgE and IgG titers to isocyanates have been associated with severe disease. 11 In subjects with positive inhalation challenges to hexamethylene diisocyanate (HDI) and diphenylmethane diisocyanate (MDI), IgG titers were more closely associated with disease than IgE titers.…”

Section: Immunologic Testing (Igg and Ige Titer) And The Evaluatiomentioning

confidence: 99%

“…Follow-up visits were conducted March 8-9, 1989; August 9-10, 1989; January 23-25, 1990; February 6-7, 1990; and May [9][10]1990. Interim reports were distributed on July 14, 1989, and February 2, 1990, and participants were notified of their own test results on December 13, 1989; March 13, 1990; and July 14,1990.…”

Section: Introductionmentioning

confidence: 99%

Health hazard evaluation report: HETA-89-083-2134, Parker Hannifin Corporation, Ravenna, Ohio.

1991

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Occurrence and specificity of IgE antibodies to isocyanates in occupationally exposed workers

Cited by 9 publications

References 21 publications

Issues in Diisocyanate Antibody Testing

Issues in Diisocyanate Antibody Testing

Hexamethylene Diisocyanate Causes Contraction of Canine Tracheal Smooth Muscles through Activation of Muscarinic Receptors

Health hazard evaluation report: HETA-89-083-2134, Parker Hannifin Corporation, Ravenna, Ohio.

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