Occurrence, Distribution, and Relative Incidence of Five Viruses Infecting Cucurbits in the State of São Paulo, Brazil

Abstract

Cucurbits species grown in 38 of 40 agricultural regions in the state of São Paulo, Brazil, were surveyed for the relative incidence of Cucumber mosaic virus (CMV), Papaya ringspot virus-type W (PRSV-W), Watermelon mosaic virus-2 (WMV-2), Zucchini lethal chlorosis virus(ZLCV), and Zucchini yellow mosaic virus (ZYMV) during May 1997 and June 1999. Samples from 621 plants, representing eight cultivated species, six wild species, and one commercial hybrid (Cucurbita moschata × C. maxima), were analyzed by plate trapped antigen enzyme-linked immunosorbent assay (PTA-ELISA). PRSV-W and ZYMV were the most frequently found viruses, accounting for 49.1 and 24.8%, respectively, of 605 samples tested. ZLCV, CMV, and WMV-2 were detected in 7.8, 6.0, and 4.5% of 612, 497, and 423 samples tested, respectively. Double infection was found in 97 samples, and triple infection was found in 10 samples. Quadruple infection was detected in one C. pepo sample. Plants that were symptomatic but negative by PTA-ELISA might be due to abiotic agents, infection by virus for which antiserum was not available, such as Squash mosaic virus, or infection with an as yet uncharacterized virus.

Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

“…Esse vírus tem sido encontrado, nos últimos anos, com alta freqüência em plantios de diferentes espécies de cucurbitáceas, estando em alguns casos, associado a severos danos na produção (4,8,9,10).…”

Occurrence and distribution of cucumber mosaic virus in cucurbits in Karanganyar, Central Java, Indonesia

“…The symptoms are typical of CMV infection in cucurbits (Zitter and Murphy, 2009). However, these symptoms can also be caused by infection with other viruses in cucurbits, such as Papaya ringspot virus (PRSV), Watermelon mosaic virus (WMV), Zucchini lethal chlorosis virus (ZLCV), Zucchini yellow mosaic virus (ZYMV) and other viruses (Ullman et al, 1991;Yuki et al, 2000;Lima et al, 2012;Romay et al, 2014;Sydanmetsa and Mbanzibwa, 2016). Based on visual observations (Table 1), it can be seen that the cucurbits crops showing symptoms of viral infections (including CMV) are widely spread in Karanganyar Regency.…”

Identification et distribution géographique des virus responsables des mosaïques chez les Cucurbitacées en Côte d’Ivoire

“…De même, Yuri et al (2000) ont signalé la présence de WMV avec un taux d'infection de l'ordre de 10,65% dans une étude menée à Sao Paulo (Brésil). Dans cette étude menée en Côte d'Ivoire, le WMV apparaît comme l'un des virus (avec le PRSV) le moins répandu dans les plantes infectées (10,6%); ce qui pourrait s'expliquer par le fait que ce virus n'est pas assez fréquent dans les zones tropicales (Shoeibi et al, 2009 (Mnari-Hattab et al, 2008), au Brésil par Yuki et al (2000). Dans d'autres cas, le titre viral pourrait être en-deçà du seuil détectable par la sérologie, donc il serait aussi opportun de faire des tests moléculaires.…”