Occurrence, Molecular Detection and Antibiotic Resistance Profile of Escherichia coli O157:H7 Isolated from Ready-to-Eat Vegetable Salads in Iran

Kouchakkhani, Hojjat; Dehghan, Parvin; Moosavy, Mir‐Hassan; Sarmadi, Bahareh

doi:10.15171/ps.2016.31

Cited by 9 publications

(12 citation statements)

References 31 publications

(39 reference statements)

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“…In the present, study 12 out of 500 (2.4%) E. coli O157:H7 was isolated from eight out of ten types of green leafy vegetable as (80%). These results were confirmed the finding of [22] who reported that Out of the 60 samples of vegetables, 10 samples (16.66%) were positive for Escherichia coli O157:H7, On the other hand [23]. found that no E. coli O157 was detected in both raw unwashed vegetables and salads also [24] did not detect E. coli O157 among all isolated E. coli form vegetables similarly [25] found that the percentages of the presence of E. coli O157 ranged from (0% to 33%) in onions and cabbage.…”

Section: B Antibiotics Susceptibility Of Isolated E Coli O157:h7supporting

confidence: 91%

The Antimicrobial Susceptibility and Detection of Virulence Genes of Escherichia coli O157:H7 Isolated from Leafy Green Vegetables

Haramain

Yagoub

2021

EJBIO

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Vegetables can be vehicles for transmission of E. coli O157:H7 to humans, therefore, this study carried out in order to investigate the presence of Enterohemorrhagic E. coli in ten different types of leafy green vegetables, determined their susceptibility to thirteen antibiotics and detected the presence of some virulence genes. Method: Five-hundred samples of green leafy vegetables namely (Lettuce, Spanish, Rocket, Parsley, Mallow, Coriander, Portulaca, Lettuce, Dill, Basil and Chard) were examined for presence of E. coli O157:H7, by using standard microbiological tests (CHROMagarTM O157:H7), further detection of E. coli O157:H7 was done by Multiplex PCR (mPCR) for the detection of virulence genes (stx1, stx2, intmin and hlyA) These genes are causative factors of settlement, adhesion, and attack of STEC bacteria to gastrointestinal mucosa. Results: E. coli O157:H7 was isolated from eight (80%) out of ten types of green leafy vegetable as 12 (2.40%) in which the highest percentage of isolation was shown in Dill and Chards samples as (4.2%), Coriander and Mallow showed percentage of isolation as (3.33% and 3.03%) respectively, Parsley, Portulaca and Lettuce showed percentages of isolation as 2.43%, 1.92%, respectively, the least percentage of isolation was shown in Rocket (1.7%), No E. coli O157:H7, was detected in Spinach and Basil. Makkah collected samples showed isolation of 7 isolates out of 12 (58.33%). All isolates were resistant to Methicillin (5µg), Metronidazole (5 µg) and Ampicillin (10 µg). Stx2 (110 bp), Stx1 (349 bp), hly A (165 bp) genes were detected. All isolates showed negative results for presence of intimin gene (890 bp). This study concluded that there is a high risk for occurrence of E. coli O157:H7 outbreaks due to consumption of the green leafy vegetables sold in Jeddah Central Market.

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Section: B Antibiotics Susceptibility Of Isolated E Coli O157:h7supporting

confidence: 91%

The Antimicrobial Susceptibility and Detection of Virulence Genes of Escherichia coli O157:H7 Isolated from Leafy Green Vegetables

Haramain

Yagoub

2021

EJBIO

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“…Of the 7 E. coli isolates examined, we found an overall prevalence of 42% (n=3) isolates showed resistance rate to AMC (29%), CIP (29%) and GEN (14%) ( Table 4). AMC, CIP and GEN resistance has been reported in studies performed worldwide, concerning RTE foods (Rasheed et al 2014;Kochakkhani et al 2016;Baloch et al 2017;Ye et al 2018). Our results were lower than those found by Kochakkhani et al (2016) (100% for AMC and CIP) and Baloch et al (2017) (80.9% for AMC and 18% for GEN).…”

Section: Discussionmentioning

confidence: 98%

“…AMC, CIP and GEN resistance has been reported in studies performed worldwide, concerning RTE foods (Rasheed et al 2014;Kochakkhani et al 2016;Baloch et al 2017;Ye et al 2018). Our results were lower than those found by Kochakkhani et al (2016) (100% for AMC and CIP) and Baloch et al (2017) (80.9% for AMC and 18% for GEN). Also, the low percentage of resistance to AMC, CIP and GEN was noted by Campos et al (2013) (5% for CIP), Lima et al (2017) (none for CIP and GEN) and Rasheed et al (2014) (8.6% for AMC).…”

Section: Discussionmentioning

confidence: 98%

“…RTE foods do not generally require serious pretreatment process and are shelf-stable, delicious, inexpensive and easily accessible to consumers (Spencer, 2005;Jaroni et al, 2010). However, these types of foods present important microbiological risk since they have been implicated as vehicles of food borne microorganisms including Escherichia coli (Ateş et al, 2011;Kochakkhani et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Antimicrobial resistance of E. coli has been traced world-wide in RTE foods (Musgrove et al, 2006;Zhao et al, 2012;Kochakkhani et al, 2016). Studies on antimicrobial resistant E. coli serotypes indicate that increasing antibiotic resistance has become a clinical and public health problem because of complicates treatment of infections caused by E. coli (Karlowsky et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Isolation, genotyping and antimicrobial susceptibility of pathogenic Escherichia coli serotypes in ready to eat foods

Karadal

Onmaz

Hızlısoy

et al. 2019

J Hellenic Vet Med Soc

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In this study, pathogenic Escherichia coli serotypes (E. coli O157:H7, O26, O111) and their molecular proximity and antimicrobial susceptibility were investigated in RTE foods. A total of 240 samples; consist of 105 stuffed mussel, 56 meatless cig kofte, 54 Russian salad, 25 cheese halva, were analyzed. The conventional culture and serotyping methods for determination of the organisms were performed and further confirmation by PCR was carried out. Confirmed E. coli O157 isolates were genotyped by the enterobacterial repetitive intergenic consensus(ERIC)-PCR. Antibacterial susceptibility testing of the isolates was performed by disc diffusion method. E. coli was detected in 7 (2.9 %) of 240 samples, including 3 (5.5%) Russian salad, 3 (2.8%) stuffed mussel, 1 (4 %) cheese halva. Two isolates from Russian salad, 1 from stuffed mussel and 1 from cheese halva were identified as E. coli O157 . In addition, stuffed mussel isolate was found to carry stx1 ve hlyA genes whereas one Russian salad isolate carried the stx1 gene. E. coli isolates were found to be resistant to amoxycillin/clavulonic acid, gentamicin and ciprofloxacin, at the rate of 29%, 14% and 29 %, respectively. Only one (14 %) isolate from stuffed mussel was classified as multidrug resistant to three antimicrobials. Furthermore, the isolates, related to O157 and O157:H7, presented different ribotypes in this study. The results provide useful data for the development of public health policy concerning the potential presence of pathogenic antimicrobial resistant E. coli serotypes in RTE foods. Strict surveillance of RTE foods at retail points for emerging pathogens, their antimicrobial resistance patterns and the potential likelihood of cross-contamination is required.

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Research advances on the contamination of vegetables by Enterohemorrhagic Escherichia coli : pathways, processes and interaction

Zhao

Sun

et al. 2022

Critical Reviews in Food Science and Nutrition

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Occurrence, Molecular Detection and Antibiotic Resistance Profile of Escherichia coli O157:H7 Isolated from Ready-to-Eat Vegetable Salads in Iran

Cited by 9 publications

References 31 publications

The Antimicrobial Susceptibility and Detection of Virulence Genes of Escherichia coli O157:H7 Isolated from Leafy Green Vegetables

The Antimicrobial Susceptibility and Detection of Virulence Genes of Escherichia coli O157:H7 Isolated from Leafy Green Vegetables

Isolation, genotyping and antimicrobial susceptibility of pathogenic Escherichia coli serotypes in ready to eat foods

Research advances on the contamination of vegetables by Enterohemorrhagic Escherichia coli : pathways, processes and interaction

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