Occurrence of antibiotics and antibiotic resistance genes in soils from wastewater irrigation areas in the Pearl River Delta region, southern China

Pan, Min; Chu, L.M.

doi:10.1016/j.scitotenv.2017.12.008

Cited by 196 publications

(53 citation statements)

References 48 publications

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“…Concentrations of sulfamethoxazole and trimethoprim were positively correlated to relative prevalence of sul3 and dfrA1, respectively, suggesting their influence on the selection of these genes. Concentration of antibiotics residue in the environment exerting a selective pressure on the acquisition of ARGs coding resistance against them are in accordance with studies of Cheng et al (2016) and Pan and Chu (2018). We found that sulfamethoxazole, ciprofloxacin and enrofloxacin selected for multiple and cross resistance.…”

Section: Discussionsupporting

confidence: 89%

Raw wastewater irrigation for urban agriculture in Africa increases the diversity of transferable antibiotic resistances genes in soil, including those encoding ESBLs

Thiele‐Bruhn²,

Ricci

et al. 2019

Preprint

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A metagenomic study was conducted to investigate the impact of raw wastewater use for irrigation in urban agriculture on the development of bacterial resistance in soil. Soil samples were collected in two African countries, from three different cities (each with irrigated and non-irrigated plots). Basic physical and chemical analysis were conducted, and the presence of selected antibiotic residues was assessed. Microbial DNA was extracted, quantified and sequenced. Microbial population structure and function, presence of horizontally transferable antibiotic resistance genes and Enterobacteriaceae plasmids replicons were analysed using bioinformatics. The relative prevalence of Proteobacteria and Bacteroidetes and sequence reads coding for microbial adaptation and growth were higher in irrigated fields; 33 and 26 transferable ARGs were found in irrigated and non-irrigated fields sequence reads, respectively. Extended spectrum β-lactam genes identified in irrigated fields included bla CARB-3, bla OXA-347, bla OXA-5 and bla Rm3. Concentration of sulfamethoxazole, ciprofloxacin and enrofloxacin in soils influenced the selection of antibiotic resistance genes encoding resistance against amphenicol, β-lactams, and tetracyclines. Ten Enterobacteriaceae plasmid amplicon groups were identified in the fields, five were common to both, two (IncW and IncP1) and three (IncY, IncFIB and IncFIA) were found in irrigated and non-irrigated fields, respectively.In conclusion, wastewater irrigation affected both soil microbial diversity and functions. Irrigated fields have more diverse transferable antibiotic resistance genes, including ESBL genes that encode resistance to βlactams antibiotics, except cephamycins and carbapenems. Even more, critical concentrations of antibiotic residues select for multiple and cross resistance. The findings from African cities show that wastewater irrigation in urban agriculture presents a serious public health risk for farmworkers and consumers by spread of bacterial resistance.

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Section: Discussionsupporting

confidence: 89%

Raw wastewater irrigation for urban agriculture in Africa increases the diversity of transferable antibiotic resistances genes in soil, including those encoding ESBLs

Thiele‐Bruhn²,

Ricci

et al. 2019

Preprint

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“…The main risk for public health is that resistance genes are transferred from environmental bacteria to human pathogens. The potential of drinking water to transport microbial pathogens to a greater number of people, causing subsequent illness, is well documented in countries at all levels of economic development [5, 6]. Furthermore, the availability of safe drinking water is an indispensible feature for preventing epidemic disease and improving the quality of life [7, 8].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Multidrug-ResistantEscherichia coliIsolated from Drinking Water Sources

Odonkor

Addo

2018

International Journal of Microbiology

109

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The control of infectious diseases is badly endangered by the rise in the number of microorganisms that are resistant to antimicrobial agents. This is because infections caused by resistant microorganisms often fail to respond to conventional treatment, resulting in prolonged illness and greater risk of death. Antimicrobial-resistant bacteria are also present in various water sources. This study therefore sought to document the microbiological quality and antibiograms of bacterial isolates (E. coli strains) from six different water sources in order to determine their safety for human consumption and to provide updated antibiotic data for pragmatic treatment of patients. Bacteria isolation and identification was done using API and conventional methods. Antibiotic susceptibility testing was conducted using the Kirby–Bauer method. Results obtained indicated that all the water sources tested were of poor quality. Bacteria isolated included E. coli, Enterobacter spp., Klebsiella spp., Salmonella typhi, Streptococcus spp., Proteus vulgaris, Vibrio cholera, Shigella spp., Pseudomonas aeruginosa, and Enterococcus faecalis. The prevalence of multidrug-resistant E. coli was 49.48%. E. coli isolates showed high resistance patterns to the tested antibiotics. They were most resistant to penicillin (32.99%), cefuroxime (28.87%), erythromycin (23.71%), and tetracycline (21.45%). In contrast, they were susceptible to nitrofurantoin (93.8%), cefotaxime and amikacin (91.75%), gentamicin (90.7%), nalidixic acid (89.65%), ciprofloxacin (74.2%), chloramphenicol (69.07%), pipemidic acid (65.97%), and cefuroxime (52.58%). Sixty-three percent (63%) of the multidrug-resistant E. coli strains recorded a multiple antibiotic resistance (MAR) index value >0.2. The susceptible antibiotics, especially the nitrofurantoin, are hence recommended in the practical treatment of waterborne bacterial diseases.

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“…The main risk for public health in water systems is that resistant genes are transferred from environmental bacteria to human pathogens. The potential of drinking water to transport microbial pathogens to a greater number of people, causing subsequent illness, is well documented in several countries at all levels of economic development [2]. The aquatic ecosystem plays a major role in the contamination and spread of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARG).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Resistance Profile of Bacteria Isolated from Boreholes and Hand Dug Wells Water in Ngaoundere Municipality of Adamawa Region in Cameroon

Tangwa¹,

Keubou²,

Nfor³

et al. 2019

AiM

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Uncontrolled uses of antibiotics have led to rapid evolution of antibiotic-resistance bacteria and antibiotic resistance gene transfer, especially in a pool of aquatic system where resistance, intermediate and susceptible bacteria to some antibiotics strive together. Consequently, there is a transfer of resistance genes. In this study, bacteria of the Enterobacteriaceae family and some gram positive bacteria isolated from some boreholes and hand dug wells water of public use were tested on 19 antibiotics of different classes. This was achieved through a disk diffusion technique to determine the antimicrobial resistance profile of the said bacteria, microbial resistance index of the drugs used (and their ability to produce Beta-lactamase). These isolates were shown to demonstrate a very high resistance to the drugs used in the area. The resistance was highest in Escherichia coli 1 (73.68%) and lowest in Streptococcus pneumoniae (47.82%). These isolates also indicated very high levels of multi-drug resistance. The minimum resistance index was 0.47, indicating that bacteria isolates were of fecal origin. It is evident from the present study that multiple antibiotic-resistant bacteria can thrive in water as an environmental reservoir, and can therefore provide a route to multidrug-resistant pathogens to enter human and animal population.

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Occurrence of antibiotics and antibiotic resistance genes in soils from wastewater irrigation areas in the Pearl River Delta region, southern China

Cited by 196 publications

References 48 publications

Raw wastewater irrigation for urban agriculture in Africa increases the diversity of transferable antibiotic resistances genes in soil, including those encoding ESBLs

Raw wastewater irrigation for urban agriculture in Africa increases the diversity of transferable antibiotic resistances genes in soil, including those encoding ESBLs

Prevalence of Multidrug-ResistantEscherichia coliIsolated from Drinking Water Sources

Antimicrobial Resistance Profile of Bacteria Isolated from Boreholes and Hand Dug Wells Water in Ngaoundere Municipality of Adamawa Region in Cameroon

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