Occurrence of bla NDM-7 within IncX3-type plasmid of Escherichia coli from India

Paul, Deepjyoti; Bhattacharjee, Amitabha; Ingti, Birson; Choudhury, Nargis Alom; Maurya, Anand Prakash; Dhar, Debadatta; Chakravarty, Atanu

doi:10.1016/j.jiac.2016.12.009

Cited by 22 publications

(13 citation statements)

References 15 publications

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“…IncX3 type is linked encoding of different NDM variants across the globe (3)(4)14). This study also reports the presence of bla NDM4 within IncX3 type plasmid.…”

Section: Discussionsupporting

confidence: 56%

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Adaptation of IncX3 plasmid encoding blaNDM-4 within broad host range: A study from India

Choudhury

Paul

Das

et al. 2019

Preprint

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Different variants of blaNDM has been reported across Indian subcontinent within diverse host range of gram negative bacilli. Most of their transferability depends on types of plasmid that encodes resistance determinant. IncX type plasmid is more recently known for carrying carbapenem resistance and NDM-4 variant is considered to have better hydrolytic property. This study aims to investigate the maintenance and stability of blaNDM-4 within BHR and transcriptional response. E.coli of clinical origin that harbor blaNDM-4 within IncX3 plasmid were used. Six different hosts were selected for gene transfer experiment, two strains of E.coli, K.pneumoniae, P.mirabilis, A.baumannii and P.aeruginosa. All the transformants and parent isolate were subjected for stability within new host. IncX3 plasmid encoding blaNDM-4 was transferred in host when imipenem (0.5µg/ml) screen agar was used for selection of transformants which are coharbouring resistance for aminoglycosides and quinolone. Plasmid was successfully expanded within all the six recipients till 55th serial passages. Transcriptional expression with NDM gene was variable for parent isolates though for new hosts it was showing random increased pattern in Proteus, E.coli, and DH5α. This could highlight, external carbapenem pressure helps in maintenance and expression of blaNDM-4 within different host range and epidemiological significance and will help in tracking the genetic vehicle responsible for their transmission,restricting their spread.

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“…IncX3 type is linked encoding of different NDM variants across the globe (3)(4)14). This study also reports the presence of bla NDM4 within IncX3 type plasmid.…”

Section: Discussionsupporting

confidence: 56%

“…across the globe with a number of cases from India (3)(4)(5). Thus this plasmid type is probably emerging as a potential genetic vehicle for lateral expression of New Delhi Mettalo beta lactamases in this subcontinent.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of IncX3 plasmid encoding blaNDM-4 within broad host range: A study from India

Choudhury

Paul

Das

et al. 2019

Preprint

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“…Pmqr genes (qnrS1,oqxAB), ESBL genes (bla TEM−52 , bla CTX−M , bla SHV −12 ), carbapenemase genes (bla NDM ) and others(12)(13). The findings as indicate that this plasmid type can carry diverse range of resistance genes in enterobacteriaceae.IncX3 type is linked encoding of different NDM variants across the globe(3)(4)14). This study also reports the presence of bla NDM4 within IncX3 type plasmid.…”

supporting

confidence: 56%

“…Inc X3 is better known for its ability to carry diverse types of resistance genes. Recently this subtype is more linked with bla NDM−4 and bla NDM−7 across the globe with a number of cases from India (3)(4)(5). Thus this plasmid type is probably emerging as a potential genetic vehicle for lateral expression of New Delhi Metalo beta lactamases in this subcontinent.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of IncX3 plasmid encoding blaNDM-4 within broad host range: A study from India

Choudhury

Paul

Das

et al. 2020

Preprint

Self Cite

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Background: Different variants of blaNDM have been reported across Indian subcontinent within diverse host range of gram negative bacilli. Most of their transferability depends on types of plasmid that encodes resistance determinant. These plasmids with external sub-inhibitory stress of antibiotics facilitate their propagation and maintenance within broad host range. IncX type plasmid is more recently known for carrying carbapenem resistance and NDM-4 variant is considered to have better hydrolytic property than other variants. Both of these factors interferes with therapeutic outcome in clinical setting. The current study was aimed to investigate the maintenance and stability of blaNDM-4 within broad host range and transcriptional response.Results: IncX3 plasmid encoding blaNDM-4 was successfully transferred in six different host when imipenem (0.5µg/ml) screen agar was used for selection of transformants. It was also found to coharbour resistance for aminoglycosides and quinolone. When checked for stability, it was observed that the plasmid was successfully expanded within all the six recipients for 55th serial passages. Transcriptional expression with IncX3 was random but at consistent level for wild type and without concentration gradient stress of imipenem. Transcriptional expression with NDM gene was variable for parent isolates though for new hosts it was showing randomly increased pattern in proteus, E.coli, and DH5α Conclusion: the present study could highlight that external carbapenem pressure helps in maintenance and expression of blaNDM-4 within different host range. This study is of epidemiological significance and will help in tracking the genetic vehicle responsible for their transmission theirby restricting their spread.

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“…International genetic contexts of bla CTX-M-14 (ISEcp1-bla CTX-M-14 -IS903D) (Lartigue, Poirel, & Nordmann, 2004) Regarding other plasmids identified in this study, IncN and IncHI2 have been related to the spread of bla CTX-M-1 and bla CTX-M-9 , respectively, and IncQ1 or IncX plasmid has been responsible by dissemination of carbapenemase encoding genes (Cerdeira et al, 2019;Mollenkopf et al, 2017;Paul et al, 2017;Zhao & Hu, 2013).…”

Section: Re Sults and Discussionmentioning

confidence: 81%

International clones of extended‐spectrum β‐lactamase (CTX‐M)‐producingEscherichia coliin peri‐urban wild animals, Brazil

Carvalho

Fernandes

Sellera

et al. 2020

Transbound Emerg Dis

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CTX‐M‐type extended‐spectrum β‐lactamase (ESBL)‐producing Escherichia coli clones have been increasingly reported worldwide. In this regard, although discussions of transmission routes of these bacteria are in evidence, molecular data are lacking to elucidate the epidemiological impacts of ESBL producers in wild animals. In this study, we have screened 90 wild animals living in a surrounding area of São Paulo, the largest metropolitan city in South America, to monitor the presence of multidrug‐resistant (MDR) Gram‐negative bacteria. Using a genomic approach, we have analysed eight ceftriaxone‐resistant E. coli. Resistome analyses revealed that all E. coli strains carried blaCTX‐M‐type genes, prevalent in human infections, besides other clinically relevant resistance genes to aminoglycosides, β‐lactams, phenicols, tetracyclines, sulphonamides, trimethoprim, fosfomycin and quinolones. Additionally, E. coli strains belonged to international sequence types (STs) ST38, ST58, ST212, ST744, ST1158 and ST1251, and carried several virulence‐associated genes. Our findings suggest spread and adaptation of international clones of CTX‐M‐producing E. coli beyond urban settings, including wildlife from shared environments.

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Occurrence of bla NDM-7 within IncX3-type plasmid of Escherichia coli from India

Cited by 22 publications

References 15 publications

Adaptation of IncX3 plasmid encoding blaNDM-4 within broad host range: A study from India

Adaptation of IncX3 plasmid encoding blaNDM-4 within broad host range: A study from India

Adaptation of IncX3 plasmid encoding blaNDM-4 within broad host range: A study from India

International clones of extended‐spectrum β‐lactamase (CTX‐M)‐producingEscherichia coliin peri‐urban wild animals, Brazil

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