Occurrence of chloroplast ribosome recognition sites within conserved elements of the RNA genomes of carlaviruses

Abstract

The nucl¢otide sequences upstream from the ,=~rlavirus olin reading frames wcrc exan~ined for direct sequence homology, Blocks of homology were evident upstream from the 25 K ORFs eF potato virus S (PVS), potato virus M (PVM} and lily symptomless virus (LSV), and upstream from th,= coast protein initiation codons of PVS, PVM, LSV. carnation latent virus and Hdenium virus S, Then,= blocks, which correspond to the Y.terminal resions of the subjenomic RNAs. were sl~own to contain potential ribosome rcco~,nitlon sequences, The distances between tit,= bindtn8 sites and initiation codons ransed from 20 to 40 nucleotides on the viral RNAs, Whilsl tit,= m.'tjority of chloroplasts mRNAs have a distance of 8 nucleotides between bindin8 site and initiation codon, the remaininB have a distance o1" 23 nucleotides which is similar to that reported here for the carlaviru~s.Subgenomic promoter: Seq nonce homology; Carlavirus; Ribosome bindin6 sit'=

Evidence that whitefly-transmitted cowpea mild mottle virus belongs to the genus Carlavirus

“…3e). Another interesting feature is the presence of the consensus sequence C/TTTAGGT upstream of the ATG codon of coat proteins of both CPMMV-S and CPMMV-M, which is similar to chloroplast ribosome binding sites observed in all carlaviruses [8,19]. These characteristics support the inclusion of CPMMV in the genus Carlavirus.…”

Host Range, Purification, and Genetic Variability in Sweet potato chlorotic fleck virus

“…A variability of even up to 81% in nucleotide sequence was documented when ordinary, Andean, and central European isolates of Potato virus S (PVS) were compared using the C-terminal part of the replicase, the CP, and the 11-kDa proteins (13,22). Due to this variability, isolate-specific primers were used to generate various regions of the PSV isolates (22); a similar situation was experienced in this study, where a successful amplification of the 3′-terminal regions of isolates KY5, TN340, and 007VIIMS was only possible with specific primers to each ( Table 2).…”

Nucleotide sequence of the carlavirus associated with blueberry scorch and similar diseases

“…Gilmer et al, 1992). Among the other motifs that have been attributed to carlavirus genomes is the presence of sequences similar to chloroplast ribosome binding sites immediately upstream from the 25K protein gene, the first gene of the triple block, and from the CP gene (Foster & Mills, 1991). Examination of this region of the BBScV genome reveals similar motifs in the same area of the BBScV genome.…”