Occurrence of Deoxynivalenol in Wheat in Slovakia during  2010 and 2011

Šliková, Svetlana; Gavurníková, Soňa; Šudyová, V.; Gregová, E.

doi:10.3390/toxins5081353

Cited by 18 publications

(13 citation statements)

References 22 publications

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“…DON concentration was measured by ELISA using the RIDASCREEN FAST DON Kit (R-Biopharm AG, Germany) [ 89 ]; its determination limit is 0.2 ppm. A calibration curve was established using 0, 0.222, 0.666, 2 and 6 ppm DON.…”

Section: Methodsmentioning

confidence: 99%

Cellular Tracking and Gene Profiling of Fusarium graminearum during Maize Stalk Rot Disease Development Elucidates Its Strategies in Confronting Phosphorus Limitation in the Host Apoplast

Zhang

Jia

et al. 2016

PLoS Pathog

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The ascomycete fungus Fusarium graminearum causes stalk rot in maize. We tracked this pathogen’s growth in wound-inoculated maize stalks using a fluorescence-labeled fungal isolate and observed that invasive hyphae grew intercellularly up to 24 h post inoculation, grew intra- and inter-cellularly between 36–48 h, and fully occupied invaded cells after 72 h. Using laser microdissection and microarray analysis, we profiled changes in global gene expression during pathogen growth inside pith tissues of maize stalk from 12 h to six days after inoculation and documented transcriptomic patterns that provide further insights into the infection process. Expression changes in transcripts encoding various plant cell wall degrading enzymes appeared to correlate with inter- and intracellular hyphal growth. Genes associated with 36 secondary metabolite biosynthesis clusters were expressed. Expression of several F. graminearum genes potentially involved in mobilization of the storage lipid triacylglycerol and phosphorus-free lipid biosynthesis were induced during early infection time points, and deletion of these genes caused reduction of virulence in maize stalk. Furthermore, we demonstrated that the F. graminearum betaine lipid synthase 1 (BTA1) gene was necessary and sufficient for production of phosphorus-free membrane lipids, and that deletion of BTA1 interfered with F. graminearum’s ability to advance intercellularly. We conclude that F. graminearum produces phosphorus-free membrane lipids to adapt to a phosphate-limited extracellular microenvironment during early stages of its invasion of maize stalk.

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Section: Methodsmentioning

confidence: 99%

Cellular Tracking and Gene Profiling of Fusarium graminearum during Maize Stalk Rot Disease Development Elucidates Its Strategies in Confronting Phosphorus Limitation in the Host Apoplast

Zhang

Jia

et al. 2016

PLoS Pathog

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“…1533.80 µg/kg), and Dobrogea (max. 1326.40 µg/kg) (Table 1 [57,64,[75][76][77][79][80][81][82]90,[128][129][130][131][132][133]. The geographic distribution of Fusarium spp.…”

Section: Deoxynivalenol Occurrence In the Triticale Crop And Other Cereals By Soil Typementioning

confidence: 99%

Deoxynivalenol Occurrence in Triticale Crops in Romania during the 2012–2014 Period with Extreme Weather Events

Gagiu

Mateescu

Dobre

et al. 2021

Toxins

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This article aims to evaluate deoxynivalenol occurrence in triticale crops in Romania in years with extreme weather events (2012: Siberian anticyclone with cold waves and heavy snowfall; 2013 and 2014: “Vb” cyclones with heavy precipitation and floods in spring). The deoxynivalenol level in triticale samples (N = 236) was quantified by ELISA. In Romania, the extreme weather events favoured deoxynivalenol occurrence in triticale in Transylvania and the southern hilly area (44–47°N, 22–25°E) with a humid/balanced-humid temperate continental climate, luvisols and high/very high risk of floods. Maximum deoxynivalenol contamination was lower in the other regions, although heavy precipitation in May–July 2014 was higher, with chernozems having higher aridity. Multivariate analysis of the factors influencing deoxynivalenol occurrence in triticale showed at least a significant correlation for all components of variation source (agricultural year, agricultural region, average of deoxynivalenol, average air temperature, cumulative precipitation, soil moisture reserve, aridity indices) (p-value < 0.05). The spatial and geographic distribution of deoxynivalenol in cereals in the countries affected by the 2012–2014 extreme weather events revealed a higher contamination in Central Europe compared to southeastern and eastern Europe. Deoxynivalenol occurrence in cereals was favoured by local and regional agroclimatic factors and was amplified by extreme weather events.

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“…Toxigenic Fusarium species are common pathogens of wheat, triticale and other cereals worldwide [ 1 , 2 , 3 ]. Many of them attack a range of plant parts and stages, such as seedlings, heads, roots, stems and ears, resulting in severe reductions of grain yield, often ranging from 10% to 40% [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Deoxynivalenol in the Gastrointestinal Tract of Immature Gilts under per os Toxin Application

Waśkiewicz

Beszterda

Kostecki

et al. 2014

Toxins

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Deoxynivalenol is also known as vomitoxin due to its impact on livestock through interference with animal growth and acceptance of feed. At the molecular level, deoxynivalenol disrupts normal cell function by inhibiting protein synthesis via binding to the ribosome and by activating critical cellular kinases involved in signal transduction related to proliferation, differentiation and apoptosis. Because of concerns related to deoxynivalenol, the United States FDA has instituted advisory levels of 5 µg/g for grain products for most animal feeds and 10 µg/g for grain products for cattle feed. The aim of the study was to determine the effect of low doses of deoxynivalenol applied per os on the presence of this mycotoxin in selected tissues of the alimentary canal of gilts. The study was performed on 39 animals divided into two groups (control, C; n = 21 and experimental, E; n = 18), of 20 kg body weight at the beginning of the experiment. Gilts received the toxin in doses of 12 µg/kg b.w./day (experimental group) or placebo (control group) over a period of 42 days. Three animals from two experimental groups were sacrificed on days 1, 7, 14, 21, 28, 35 and 42, excluding day 1 when only three control group animals were scarified. Tissues samples were prepared for high performance liquid chromatography (HPLC) analyses with the application of solid phase extraction (SPE). The results show that deoxynivalenol doses used in our study, even when applied for a short period, resulted in its presence in gastrointestinal tissues. The highest concentrations of deoxynivalenol reported in small intestine samples ranged from 7.2 (in the duodenum) to 18.6 ng/g (in the ileum) and in large intestine samples from 1.8 (in transverse the colon) to 23.0 ng/g (in the caecum). In liver tissues, the deoxynivalenol contents ranged from 6.7 to 8.8 ng/g.

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Occurrence of Deoxynivalenol in Wheat in Slovakia during 2010 and 2011

Cited by 18 publications

References 22 publications

Cellular Tracking and Gene Profiling of Fusarium graminearum during Maize Stalk Rot Disease Development Elucidates Its Strategies in Confronting Phosphorus Limitation in the Host Apoplast

Cellular Tracking and Gene Profiling of Fusarium graminearum during Maize Stalk Rot Disease Development Elucidates Its Strategies in Confronting Phosphorus Limitation in the Host Apoplast

Deoxynivalenol Occurrence in Triticale Crops in Romania during the 2012–2014 Period with Extreme Weather Events

Deoxynivalenol in the Gastrointestinal Tract of Immature Gilts under per os Toxin Application

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