Occurrence of emerging food-borne pathogenic Arcobacter spp. isolated from pre-cut (ready-to-eat) vegetables

Mottola, Anna; Bonerba, Elisabetta; Bozzo, Giancarlo; Marchetti, Patrizia; Celano, Gaetano Vitale; Colao, Valeriana; Terio, Valentina; Tantillo, Giuseppina; Figueras, María José; Pinto, Angela Di

doi:10.1016/j.ijfoodmicro.2016.07.012

Cited by 45 publications

(46 citation statements)

References 61 publications

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“…The present study evaluated and compared the presence and variability of putative virulence genes in A. butzleri isolates from three environmental sites belonging to the dairy chain. Considering all three different environmental sites, the most commonly detected genes were ciaB (100%), mviN (98%), tlyA (98%), cj1349 (97%), pldA(97%) and cadF (96%), in accordance with other studies (Douidah et al 2012;Karadas et al 2013;Ferreira et al 2014;Tabatabaei et al 2014;Girbau et al 2015;Zacharow et al 2015;Laishram et al 2016;Mottola et al 2016) investigating isolates of A. butzleri from different hosts and environments like human stool, food products, processing water, faecal samples, slaughterhouse processing line equipment, with prevalence values ranging from 66 to 100%.…”

Section: Resultssupporting

confidence: 89%

“…; Mottola et al . ) but very similar to that reported in the only study performed on dairy isolates (Girbau et al . ).…”

Section: Discussionsupporting

confidence: 90%

“…; Mottola et al . ) investigating isolates of A. butzleri from different hosts and environments like human stool, food products, processing water, faecal samples, slaughterhouse processing line equipment, with prevalence values ranging from 66 to 100%.…”

Section: Discussionmentioning

confidence: 99%

“…isolated from precut vegetables and the presence of virulence‐associated genes (Mottola et al . ). Some studies showed a different prevalence of putative virulence genes in relation to the source of the strain (Girbau et al .…”

Section: Introductionmentioning

confidence: 97%

“…isolates worldwide: data are available from Belgium, Germany, Iran, Portugal and Spain (Lehmann et al 2015;Girbau et al 2015;Ferreira et al 2014;Tabatabaei et al 2014;Karadas et al 2013;Levican et al 2013;Douidah et al 2012) and only recently from Italy in a study designed to evaluate the occurrence of Arcobacter sp. isolated from precut vegetables and the presence of virulence-associated genes (Mottola et al 2016). Some studies showed a different prevalence of putative virulence genes in relation to the source of the strain (Girbau et al 2015;Lehmann et al 2015;Douidah et al 2011), but most investigations focused on clinical human or animal isolates, poultry and poultry meat isolates, fish meat and shellfish isolates and pork isolates.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

et al. 2017

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Section: Resultssupporting

confidence: 89%

“…; Mottola et al . ) but very similar to that reported in the only study performed on dairy isolates (Girbau et al . ).…”

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

et al. 2017

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Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Lameei

Rahimi

Shakerian

et al. 2022

Veterinary Medicine & Sci

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Background Arcobacter spp. has been considered an emerging foodborne pathogen and a hazard to human health. The dairy chain has been isolated from different sources; nevertheless, data on Arcobacter occurrence in raw milk and dairy products in Iran are still scant. Objective The present study investigates the prevalence, antimicrobial susceptibility and the presence of virulence genes of Arcobacters species isolated from milk and dairy products. Methods Then, a total of 350 raw milk samples and 400 dairy product samples were collected from dairy supply centers in Isfahan, Iran. Presumptive Arcobacter strains were obtained by enriching samples in Oxoid Arcobacter enrichment broth (AEB) followed by the filtration of enrichment product through 0.45‐μm pore size membrane filters laid onto non‐selective blood at 30°C under microaerophilic conditions. Molecular identification of Arcobacter cryaerophilus and A. butzleri was performed by Polymerase chain reaction (PCR) amplification of the 16S rRNA gene, followed by sequencing. The disc diffusion method was used to determine the antimicrobial susceptibility of isolates. Targeted resistance and virulence genes were detected using multiplex PCR. Results The results show a low recovery rate of Arcobacter spp. in milk. Arcobacters were found in all types of milk, except raw camel milk, but were absent from all dairy products. Arcobacter butzleri was the predominant species in raw milk. Detection of virulence genes shows that all virulence genes targeted were found among A. butzleri, and six (cadF, cj1349, irgA, mviN, pldA, tlyA) were found among A. cryaerophilus. All A. butzleri strains and some A. cryaerophilus strains isolated from milk were resistant to amoxicillin‐clavulanic acid and tetracycline. All A. cryaerophilus isolates from milk were susceptible to gentamycin, streptomycin, erythromycin and ciprofloxacin. The distribution of resistance genes in Arcobacter strains in milk shows that all isolates carried tet(O) and blaOXA‐61 genes. Conclusions In conclusion, the results indicate a low recovery rate of Arcobacter spp. in milk and milk products. However, a significant number of Arcobacter strains with putative virulence genes may be potential pathogens for humans and an overall increase in Arcobacter resistance to first‐line antibiotics. These results highlight the need for regular surveillance of Arcobacter strains in milk and milk products in Iran.

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Emerging and Reemerging Bacterial Pathogens of Humans in Environmental and Hospital Settings

Fono-Tamo

Chukwu

Ubomba-Jaswa

et al. 2020

Current Microbiological Research in Africa

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Occurrence of emerging food-borne pathogenic Arcobacter spp. isolated from pre-cut (ready-to-eat) vegetables

Cited by 45 publications

References 61 publications

Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

Occurrence of putative virulence genes onArcobacter butzleriisolated from three different environmental sites throughout the dairy chain

Genotyping, antibiotic resistance and prevalence of Arcobacter species in milk and dairy products

Emerging and Reemerging Bacterial Pathogens of Humans in Environmental and Hospital Settings

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