Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Wasiński, Bernard; Różańska, H.; Osek, Jacek

doi:10.2478/bvip-2013-0089

Cited by 12 publications

(7 citation statements)

References 28 publications

(28 reference statements)

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“…Wasiński et al [38] have revealed the number of cephalosporin resistance occurring on farms. The incidence of resistance to cephalosporins has been widely reported and has a lot to do with pig farming [39].…”

Section: Resultsmentioning

confidence: 99%

Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang , Indonesia

Kallau¹,

Wibawan²,

Lukman³

et al. 2018

J Adv Vet Anim Res

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Objective: The purpose of this study was to detect the incidence of multi-drug resistant (MDR) and the spread of tet genes that encode tetracycline (TE) resistance in E. coli in pig farms in the city of Kupang, Indonesia. Materials and Methods: Samples of pig feces have been obtained from 96 pig farms in Kupang city, Indonesia. Escherichia coli bacteria were isolated and identified morphologically and biochemically, and finally confirmed by the API test. The disk diffusion method has been used to observe the antibiotic sensitivity effects and has been followed by observing resistant genes encoding TE resistance using the multiplex polymerase chain reaction (m-PCR) method to detect the presence of tet genes such as tet (A), tet (B), tet (C), tet (D), and tet (E), respectively. Results: A total of 82 (85.4%) of E. coli isolates have been found in all pig feces samples obtained from 96 pig farms in Kupang city. This study has shown a high level of antibiotic resistance dominated by erythromycin (85.4%) and cephalothin (58.5%) and followed by several other antibiotics with a percentage below 34.1%. The prevalence of MDR E. coli was 57.3% by showing 39 different patterns. The most common pattern was showed by the Cephalothin-Colistin-Erythromycin pattern. The resistance of E. coli to TE appears to be related to the presence of tet (A) and tet (E) genes. Conclusion: This study has encouraged the need for public awareness (farmers) of the wise use of antibiotics in preventing the spread of resistant bacteria that can cause health problems in animals and humans.

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Section: Resultsmentioning

confidence: 99%

Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang , Indonesia

Kallau¹,

Wibawan²,

Lukman³

et al. 2018

J Adv Vet Anim Res

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“…This result is lower than 9.4% isolation prevalence of ESBLs-producing E. coli reported by Chah and Oboegbulem (2007) among 171 ampicillinresistant E. coli isolates from chicken in Nigeria. Smet et al (2008), Geser et al (2011), Garcia-Graells et al (2010 and Wasinski et al (2013) reported 60, 92.3, 42 and 11.7% isolation prevalence of ESBLs-producing E. coli in poultry carcasses in Belgium, broilers in Belgium, pigs and cattle in Switzerland, and meat samples in Poland, respectively. In the Netherlands, Overdevest et al (2011) and Leversteinvan Hall et al (2011) reported 80 and 94% prevalence of ESBLs-producing E. coli in chicken meat and raw meat, respectively.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

et al. 2015

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This study was conducted to isolate generic extended-spectrum β-lactam (ESBL)-resistant enterobacteria from pigs reared in Enugu State Southeast, Nigeria and determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 190, randomly selected, apparently healthy pigs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 2 µg/ml of cefotaxime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 46 ESBL-resistant enterobacterial isolates, 4 (8.7%) were Escherichia coli, 11 (23.9%) were Salmonella species, while 31 (67.4%) were Klebsiella species. Resistance of the Salmonella isolates was 45.5% to ciprofloxacin, 36.4% to ofloxacin and levofloxacin, 9.1% to norfloxacin, amikacin and gentamicin, 27.3% to streptomycin, 72.7% to chloramphenicol and 90.9% to tetracycline. Resistance of the Klebsiella isolates was 93.5% to ampicillin, 12.9% to ciprofloxacin, 19.4% to ofloxacin and levofloxacin, 9.7% to norfloxacin and streptomycin, 64.5% to chloramphenicol and 38.7% to tetracycline. Resistance of the E. coli isolates was 100% to gentamicin, 75% to ampicillin and streptomycin, 50% to ciprofloxacin, norfloxacin, chloramphenicol and tetracycline, and 25% to ofloxacin, levofloxacin and amikacin. All the isolates were resistant to ceftriaxone, cefotaxime, ceftazidime, cefepime, cefpodoxime, amoxicillin/clavulanic acid and aztreonam. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 54.8% for Klebsiella, 90.9% for Salmonella and 100% for E. coli, respectively. This study has shown that pigs reared in Enugu State Southeast, Nigeria, are colonized by ESBL-resistant Enterobactericeae and are potential reservoirs and disseminators of these organisms.

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“…The group of 18 ESBLproducing and 7 AmpC-producing E. coli isolated from 141 meat samples, including 78 samples of pork, 44 samples of beef, and 19 samples of poultry (Gallus gallus), was investigated. The production of β-lactamases was determined earlier by the use of disk test D68C (Mast Diagnostica, Germany), confirmatory MIC test Sensititer ® ESBL (Trek Diagnostic System, UK), and by polymerase chain reaction (PCR) as previously described (20). (12).…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial resistance of ESBLand AmpC-producing Escherichia coli isolated from meat

Wasiński

Różańska

Osek

2014

Bulletin of the Veterinary Institute in Pulawy

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In the present study, 25 Escherichia coli strains isolated from beef, pork, and poultry meat, and producing extendedspectrum β-lactamases (ESBL) (18 strains) or AmpC-cephalosporinases (7 strains) were tested for antimicrobial resistance using the minimum inhibitory concentration method with 16 antimicrobial agents. All examined strains were resistant to ampicillin and the first-generation cephalosporins. Variable resistance to the third-generation cephalosporins (40%-100% among ESBLproducing strains and 0-72% among AmpC-producing strains) was noted. Less than 30% of examined strains were resistant to ciprofloxacin. All isolates were susceptible to the fourth-generation cephalosporins, cephalosporins connected with inhibitors of β-lactamases, carbapenems, and gentamycin.

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Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Cited by 12 publications

References 28 publications

Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang , Indonesia

Detection of multi-drug resistant (MDR) Escherichia coli and tet gene prevalence at a pig farm in Kupang , Indonesia

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Antimicrobial resistance of ESBLand AmpC-producing Escherichia coli isolated from meat

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