Occurrence of Honeysuckle Yellow Vein Virus (HYVV) containing a monopartite DNA-A genome in Korea

Abstract

Two newly emerged begomoviruses were isolated from naturally infected tomato (Solanum lycopersicum) plants grown in greenhouses at Jeju Island and Dangjin in Korea and their genomes were characterized. These viruses-infected plants had very small leaves that curled upward, yellow margins and a leathery appearance, and a bushy and stunted appearance with short internodes. Nucleotide (nt) sequence analysis of their genomes showed that they have a DNA-A component of a monopartite begomovirus. Their genomes comprised 2763 and 2764 nucleotides with six open reading frames. The results of nt sequence similarity analysis of DNA-A genome between the two Korean isolates and isolates of Tobacco leaf curl Japan virus (TbLCJV), Honeysuckle yellow vein virus (HYVV), Honeysuckle yellow vein mosaic virus (HYVMV), and Eupatorium yellow vein virus in Japan (EpYVV) showed that they are likely similar to HYVV-[Masuda] (89.4-92.8% nt identity). Consequently, we tentatively propose the two isolates' names as HYVV-Jeju and -DJ according to the ICTV geminivirus rules. Phylogenetic relationship analysis of 33 DNA-A genome sequences using PAUP* 4.0b10 and MrBayes revealed that HYVV-Jeju and -DJ belong to the Far East Asian begomovirus species complex. Within the Far East Asian begomovirus species complex, HYVV-Jeju and -DJ are distantly related to EpYVV, HYVMV, and TbLCJV groups. Based on the presence of a recombination fragment spanning the C3 ORF, a recombinant origin was suggested for both HYVV-Jeju and -DJ, with parents close to Japanese isolates HYVMV-[SP1:00] and Eupatorium yellow vein virus (EpYVV)- [Suya]. In addition, the presence of a further recombination fragment spanning the IR suggested the parents of HYVV-DJ were close to HYVV-Jeju and EpYVV- [Suya].

Begomoviruses and Their Emerging Threats in South Korea: A Review

“…2. Very recently, four begomovirus research articles have appeared Lee et al, 2010;Lee et al, 2011;Wang et al, 2011). In a phylogenetic analysis of complete nucleotide sequences (genome) as well as individual ORFs of Korean TYLCV isolates with Far East Asian begomoviruses and also across the world, all the Korean TYLCV isolates showed highest sequence identity and clustered with the Japan TYLCV isolates.…”

Symptom Severity of Nicotiana benthamiana Plants Inoculated with Agrobacterium Containing Infectious DNA-A Clones of Honeysuckle Yellow Vein Virus (HYVV)

“…In Korea, tomato (Solanum lycopersicum)-infecting begomoviruses (family Geminiviridae) have become widely spread nationwide over the years [1][2][3]. Among these viruses, Honeysuckle yellow vein virus (HYVV) infects tomato plants, causing symptoms such as small leaves showing yellowish edge and plants showing short internodes and stunted growth [2]. HYVV also induces yellow vein symptoms accompanied by oval-shaped enations on the bottom side of leaves of the natural host, Lonicera japonica (honeysuckle) [4].…”

“…HYVV also induces yellow vein symptoms accompanied by oval-shaped enations on the bottom side of leaves of the natural host, Lonicera japonica (honeysuckle) [4]. Genome sequences of HYVV have been reported from the United Kingdom, Japan and Korea [2,5,6]. In Korea, two HYVV isolates, HYVV- [ were identified from tomato plants [2], while a HYVV isolate, HYVV- [Kr] (accession number GQ477135), was identified from symptomatic leaves of L. japonica [5].…”

“…Genome sequences of HYVV have been reported from the United Kingdom, Japan and Korea [2,5,6]. In Korea, two HYVV isolates, HYVV- [ were identified from tomato plants [2], while a HYVV isolate, HYVV- [Kr] (accession number GQ477135), was identified from symptomatic leaves of L. japonica [5]. The analysis of HYVV-[Jeju] and HYVV- [DJ] revealed that they are distantly related to known HYVV isolates.…”

“…The analysis of HYVV-[Jeju] and HYVV- [DJ] revealed that they are distantly related to known HYVV isolates. Interestingly, HYVV-[Jeju] and HYVV- [DJ] are not associated with betsatellites [2], while HYVV-[Kr] is associated with betasatellites (accession number GQ495268) [5]. In addition, HYVV- [Jeju] and - [DJ] are close to HYVV-[Masuda] (89.4-92.8% nt identity), while HYVV-[Kr] is close to HYVV-[UK2] based on nucleotide (nt) sequence homology and phylogenetic tree analysis.…”