Occurrence of Fusarium oxysporum causing wilt on pepper in Mexico

Velarde‐Félix, Sixto; Garzón‐Tiznado, José Antonio; Hernández-Verdugo, Sergio; Orona, Carlos Alfonso López; Retes‐Manjarrez, Jesús Enrique

doi:10.1080/07060661.2017.1420693

Cited by 29 publications

(16 citation statements)

References 28 publications

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“…The pathogens were identified as Phytophthora capsici L. (identified using the method reported by Mitchell and Kannwischer-Mitchell, 1995 and the PCR strategy for species as reported by Silvar et al, 2005 ), Fusarium oxysporum F. Sp. capsici ( Velarde-Félix et al, 2018 ), and Rhizoctonia solani (anastomosis group GA4, Montero-Tavera et al, 2013 ), provided by the Molecular Markers Laboratory of the National Institute of Agricultural and Livestock Forest Research (INIFAP) Campo Experimental Bajío.…”

Section: Methodsmentioning

confidence: 99%

“…The pathogens were identified as Phytophthora capsici L. (identified using the method reported by Mitchell and Kannwischer-Mitchell, 1995 and the PCR strategy for species as reported by Silvar et al, 2005), Fusarium oxysporum F. Sp. capsici (Velarde-Félix et al, 2018), and Rhizoctonia solani Extraction of Genomic DNA From Phytophthora capsici L, Fusarium oxysporum, and Rhizoctonia solani Five disks (8 mm diameter) of potato dextrose agar medium with mycelium of each pathogen were placed in potato dextrose broth medium acidified with lactic acid at pH 4.0. Growth of the pathogens was carried out using incubation at 28 • C, 180 rpm for 4 days for P. capsici and R. Solani.…”

Section: Biological Materialsmentioning

confidence: 99%

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Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

et al. 2021

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Chili pepper (Capsicum annuum L.) production is affected by wilt and root rot, the most devastating disease caused by the pathogen complex of oomycete Phytophthora capsici Leon and the fungi Fusarium oxysporum Schlecht and Rhizoctonia solani Kühn, infecting roots, stems, leaves, and fruits. Fungicides are currently inefficient against this disease and have a high environmental impact. The use of elicitors is a sustainable alternative for inducing resistance to wilting and root rot. DNA fragments of an organism’s own origin (conspecific or self-DNA) have shown the ability to inhibit growth and activate defense mechanisms in some plant species. In this investigation, the effect of the fragmented DNA mixture of Phytophthora capsici L., Fusarium oxysporum S., and Rhizoctonia solani K. on the protection against wilt and root rot of Capsicum annuum L. plants was evaluated. Changes in plant performance, phenolics, and flavonoids contents, as well as gene expression involved in the production of defense metabolites after the fragmented and unfragmented DNA mixture in three concentrations (20, 60, and 100 μg mL–1) in chili peppers, were studied. The results obtained showed a decrease in plant height in 60 and 100 μg mL–1 concentrations in absence of pathogens. Moreover, the treatment with fragmented DNA 100 μg mL–1 showed significant increase in the content of phenolic compounds and total flavonoids as well as gene expression associated to plant defense in comparison with control plants. Interestingly, foliar application of DNA fragments of the pathogen complex to a concentration of 100 μg mL–1 caused a 40% decrease in the mortality of infected plants with the pathogens at 30 days post-inoculation compared with control plants inoculated with the pathogen complex but not sprayed with DNA fragments. These results suggested a perspective for application of fragmented DNA of these pathogens at the agricultural level in crop protection strategies to cope with wilt and root rot in Capsicum.

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Section: Methodsmentioning

confidence: 99%

“…The pathogens were identified as Phytophthora capsici L. (identified using the method reported by Mitchell and Kannwischer-Mitchell, 1995 and the PCR strategy for species as reported by Silvar et al, 2005), Fusarium oxysporum F. Sp. capsici (Velarde-Félix et al, 2018), and Rhizoctonia solani Extraction of Genomic DNA From Phytophthora capsici L, Fusarium oxysporum, and Rhizoctonia solani Five disks (8 mm diameter) of potato dextrose agar medium with mycelium of each pathogen were placed in potato dextrose broth medium acidified with lactic acid at pH 4.0. Growth of the pathogens was carried out using incubation at 28 • C, 180 rpm for 4 days for P. capsici and R. Solani.…”

Section: Biological Materialsmentioning

confidence: 99%

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

et al. 2021

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“…In pepper ( Capsicum spp.) crops, serious post-harvest losses are caused by F. oxysporum [ 2 ]. Peppers from about 35 Capsicum species are consumed, most widely from C. annuum , C. baccatum , C. frutescens , C. pubescens and C. chinense, which have been the most successfully domesticated and cultivated [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense

et al. 2020

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Fusarium equiseti strain FCHE and Fusarium oxysporum strain FCHJ were isolated from the roots of wilting habanero pepper (Capsicum chinense Jacq.) seedlings with root rot. Toward developing a biorational control of these serious phytopathogenic strains, ethanolic (EE) and aqueous (AE) extracts of different vegetative parts of 40 tropical native plants of the Yucatán Peninsula were screened for antifungal activity. Extracts of six out of 40 assayed plants were effective, and the most inhibitory extracts were studied further. EEs from Mosannona depressa (bark from stems and roots), Parathesis cubana (roots), and Piper neesianum (leaves) inhibited mycelial growth of both strains. Each active EE was then partitioned between hexane and acetonitrile. The acetonitrile fraction from M. depressa stem bark (MDT-b) had the lowest minimum inhibitory concentration of 1000 µg/mL against both pathogens and moderate inhibitory concentration (IC50) of 462 against F. equiseti and 472 µg/mL against F. oxysporum. After 96 h treatment with EE from M. depressa stem bark, both strains had distorted hyphae and conidia and collapsed conidia in scanning electron micrographs. Liquid chromatography–ultraviolet–high resolution mass spectrometry analysis revealed that the major component of the fraction was α-asarone. Its antifungal effect was verified using a commercial standard, which had an IC50 of 236 µg/mL against F. equiseti and >500 µg/mL against F. oxysporum. Furthermore, the P. cubana hexane fraction and P. neesianum acetonitrile fraction had antifungal activity against both Fusarium pathogens. These compounds provide new options for biorational products to control phytopathogenic fungi.

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“…or Rhizoctonia sp. (Anaya-López et al, 2011;Rivera-Jiménez et al, 2018;Velarde-Félix et al, 2018). The objectives of this study were to identify and morphologically characterize the isolates obtained from cucurbitaceae and solanaceae crops causing "wilt" in Jalisco and Michoacán, Mexico.…”

Section: Fully Bilingualmentioning

confidence: 99%

Morphological characterization of Phytophthora capsici isolates from Jalisco and Michoacán, Mexico

Reyes-Tena

Rodríguez-Alvarado

Pedraza‐Santos

et al. 2020

RMF

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Phytophthora capsici es el principal fitopatógeno del cultivo de chile (Capsicum annuum) y de diversas plantas de interés comercial en México. El desconocimiento por parte de los productores sobre la presencia de este patógeno en zonas de cultivo dificulta la prevención y manejo de la enfermedad. El objetivo de este trabajo fue identificar y caracterizar morfológicamente aislados obtenidos de cultivos de cucurbitáceas y solanáceas con síntomas de “marchitez” en Jalisco y Michoacán, México. Los muestreos se realizaron durante 2016 y 2017. Los 41 aislados de P. capsici obtenidos de plantas enfermas se analizaron por morfología comparativa con base a caracteres sexuales y asexuales. Se caracterizaron 33 aislamientos del cultivo de C. annuum, seis de C. pepo y dos de S. lycopersicum. La mayoría de los aislados presentaron características morfológicas típicas de P. capsici. Solo un aislado presentó clamidosporas, globosas y terminales (aislado de Queréndaro, Mich). Se registraron 40 aislados heterotálicos y un aislado homotálico. Se determinó la patogenicidad de siete aislados, asociando a P. capsici como el agente causal de la marchitez para estos aislados y para siete analizados en un estudio previo. Los 27 aislamientos restantes solo se asociaron a la enfermedad. Se sugiere realizar un manejo integrado de este patógeno en las zonas de producción de Jalisco y Michoacán.

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Occurrence of Fusarium oxysporum causing wilt on pepper in Mexico

Cited by 29 publications

References 28 publications

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

Effect of Fragmented DNA From Plant Pathogens on the Protection Against Wilt and Root Rot of Capsicum annuum L. Plants

Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense

Morphological characterization of Phytophthora capsici isolates from Jalisco and Michoacán, Mexico

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