Occurrence of <i>Vibrio parahaemolyticus</i> in selected marine invertebrates, sediment, and seawater around Alexandria, Egypt

El-Sahn, Malak A.; El‐Banna, A. A.; Shehata, Ahmed

doi:10.1139/m82-187

Cited by 11 publications

(8 citation statements)

References 3 publications

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“…2). Although the seasonal distribution of V. parahaemolyticus in the bay waters shown in this paper was similar to other ecological reports including ours, density of the vibrios seemed to be higher compared to that of other studied environments (3,11,12,15,17,32). Higher density recovered by replica method than that by MPN method was more significant in the warmer season (Fig.…”

Section: Month Of Aampllngsupporting

confidence: 89%

“…parahaemolyticus and many other vibrios become abundant during warmer months in seawaters of Japan (12,24) and other parts of the world but in cold weather they are rarely isolated (12,14,17,22,24). V. parahaemolyticus has frequently been isolated from patients with food poisoning and has been found to account for about 20 to 30% of all food poisoning cases in Japan (18).…”

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confidence: 99%

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Analysis of Seawaters for the Recovery of Culturable Vibrio parahaemolyticus and Some Other Vibrios

Alam

Tomochika

Miyoshi

et al. 2001

Microbiology and Immunology

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Abstract:We investigated the recovery of dormant and injured cells along with the normally culturable cells of Vibrio species with special emphasis on V. parahaemolyticus using both selective and non-selective media at moderate (20 C) and standard (37 C) culture temperatures from a bay water environment. Culture temperatures (20 or 37 C) did not affect the recovery of V.parahaemolyticus but did for other vibrios. We observed similar seasonality of V. parahaemolyticus as in most other environmental studies. V. parahaemolyticus and other Vibrio species were recovered in higher numbers by a replica plating method compared to most probable number (MPN) and direct TCBS (thiosulfate citrate bile-salt sucrose) agar counts. Even with the replica plating method, however, vibrios number goes down to a minimum level and V. parahaemolyticus was undetectable during the cool temperature period of the year, although total bacterial cells and CFU on nutrient agar (with 2% NaCI) did not vary so much during the study period.

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Section: Month Of Aampllngsupporting

confidence: 89%

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confidence: 99%

Analysis of Seawaters for the Recovery of Culturable Vibrio parahaemolyticus and Some Other Vibrios

Alam

Tomochika

Miyoshi

et al. 2001

Microbiology and Immunology

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“…Whatever the factors, detection of tdh trh gene positive V. parahaemolyticus strains 4600 per 100 ml of water. Although seasonal distribution of V. parahaemolyticus in the waters of the Seto-Inland Sea shown in this report was similar to that reported in other ecological studies including ours, the density of V. parahaemolyticus seemed to be higher in Kojima Bay (area A) compared to that in other studied environments (Barbieri et al,1999;Caruso et al, 1998;Chowdhury et al, 1990;Depaola et al, 1990;EI-Sahn et al, 1982;Suthienkul et al, 1995;Watkins and Cabelli, 1985). Such higher density of V. parahaemolyticus during the summer period was also evidenced in our recent study at the Kojima Bay site (Alam et al, 2001).…”

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Abundance of Vibrio parahaemolyticus having tdh and/or trh genes in an area of Seto-Inland Sea, Japan.

et al. 2002

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Vibrio parahaemolyticus is an important etiological agent of gastroenteritis associated with seafood consumption in Japan and many parts of the world. Thermostable direct hemolysin (TDH)and TDH-related hemolysin (TRH), encoded by the tdh and trh genes respectively, are the most recognized pathogenic factors of this bacterium. During this study a high percentage of V. parahaemolyticus isolated from an area was found to be positive for the tdh and/or trh genes by PCR, although almost all of the isolates lacked the ability to produce active hemolysin. Such tdh and/or trh positive strains were isolated from a specific coastal area, but not isolated from four other areas of the Seto-Inland Sea. This particular area, Kojima Bay, receives freshwater from several adjacent rivers, and such influx of water may have special effects on the growth V. parahaemolyticus, as evidenced by the high density of this bacterium in water samples (2300 to 4600 per 100 ml). V. parahaemolyticus of various sero-groups were isolated from each area; however, Kojima Bay samples were dominated by 01, 03, and 04 sero-groups.

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“…counts are much higher than those for overlying waters (3,5,7,8). Thus, the objectives of this study were to compare Pacific, Gulf, and Atlantic coast waters and oysters for densities of V. parahaemolyticus; to correlate V. parahaemolyticus counts in oysters and water with water salinity, temperature, and bacteriological parameters; and to determine the virulence markers of selected V. parahaemolyticus isolates.…”

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Incidence of Vibrio parahaemolyticus in U.S. coastal waters and oysters

DePaola

Hopkins

Peeler

et al. 1990

Appl Environ Microbiol

203

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Island which had been designated as approved or prohibited by the National Shellfish Sanitation Program. Fecal coliforms counts, aerobic plate counts, and Vibrio parahaemolyticus densities were determined for the samples. Mean V. parahaemolyticus density was more than 100 times greater in oysters than in water, whereas density of fecal coliforms was approximately 10 times higher in oysters. Seasonal and geographical distributions of V. parahaemolyticus were related to water temperature, with highest densities in samples collected in the spring and the summer along the Gulf coast. The synthetic DNA probe for thermostable direct hemolysin hybridized with 2 of 50 isolates, 1 of which was positive by the Kanagawa test. Vibrio parahaemolyticus is an enteric pathogen transmitted to humans primarily through consumption of raw or mishandled seafood (2, 23). Like other members of the genus Vibrio, it is a gram-negative, halophilic bacterium that occurs naturally in estuarine environments (15). Its distribution is worldwide, but reported densities of V. parahaemolyticus in the environment and in seafood vary greatly according to season, location, sample type, fecal pollution, and analytical methodology (3, 5, 8, 16, 27). The public health significance of V. parahaemolyticus contamination in seafoods is debatable, since few environmental or seafood isolates produce the thermostable-direct hemolysin (TDH) or Kanagawa hemolysin associated with most (96.5%) pathogenic strains found among clinical isolates (12, 20, 21). However, a TDH-related hemolysin has been isolated from Kanagawa phenomenon-negative V. parahaemolyticus strains associated with gastroenteritis in Southeast Asia (13). In an evaluation (5) of four methods for the enumeration of V. parahaemolyticus in seawater and oysters, we found that the hydrophobic grid membrane filter method described by Entis and Boleszczuk (9) gave the highest density estimates. This method had been used to evaluate the effects of thermal stress on artificially contaminated seafoods but had not been used in environmental surveys. Past V. parahaemolyticus surveys were conducted regionally but did not compare distribution of the organism among the Pacific, Gulf, and Atlantic coasts. The present study reports the incidence of V. parahaemolyticus in U.S. oysters and seawater as determined by the hydrophobic grid membrane filter method. The National Shellfish Sanitation Program classification of shellfish-growing areas is based on fecal coliform densities in the overlying waters (14); however, a statistical evaluation is needed to correlate V. parahaemolyticus densities in shellfish with either fecal coliform or V. parahaemolyticus counts for the overlying waters. In shellfish, V. parahaemolyticus

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Occurrence of Vibrio parahaemolyticus in selected marine invertebrates, sediment, and seawater around Alexandria, Egypt

Cited by 11 publications

References 3 publications

Analysis of Seawaters for the Recovery of Culturable Vibrio parahaemolyticus and Some Other Vibrios

Analysis of Seawaters for the Recovery of Culturable Vibrio parahaemolyticus and Some Other Vibrios

Abundance of Vibrio parahaemolyticus having tdh and/or trh genes in an area of Seto-Inland Sea, Japan.

Incidence of Vibrio parahaemolyticus in U.S. coastal waters and oysters

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