Occurrence of iris yellow spot virus from onion crops in Egypt

Hafez, Elsayed E.; El-Morsi, A. A.; El-Shahaby, O. A.; Abdelkhalek, Ahmed

doi:10.1007/s13337-014-0235-7

Cited by 27 publications

(18 citation statements)

References 17 publications

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“…In the protective assay, the upper-right halves of the leaves were treated with H. tuberculatum WPE 24 h before mechanical viral inoculation. In contrast, the upper-left halves of the leaves were inoculated with TMV only without any treatment [ 54 , 55 ]. Mock leaves treated with a mixture of equal volumes of DMSO, sterile distilled H 2 O, and phosphate buffer with carborundum were used as the controls.…”

Section: Methodsmentioning

confidence: 99%

The Phytochemical, Antifungal, and First Report of the Antiviral Properties of Egyptian Haplophyllum tuberculatum Extract

Abdelkhalek

Salem

Hafez

et al. 2020

Biology

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In this study, ethanol whole plant extract (WPE) of Haplophyllum tuberculatum was characterized and tested for its antifungal and antiviral activities against Fusarium culmorum, Rhizoctonia solani and tobacco mosaic virus (TMV). High Performance Liquid Chromatography (HPLC) analysis showed that the main phytochemical constituents of H. tuberculatum WPE were resveratrol (5178.58 mg/kg), kaempferol (1735.23 mg/kg), myricetin (561.18 mg/kg), rutin (487.04 mg/kg), quercetin (401.04 mg/kg), and rosmarinic acid (387.33 mg/kg). By increasing H. tuberculatum WPE at concentrations of 1%, 2%, and 3%, all of the fungal isolates were suppressed compared to the two positive and negative controls. Under greenhouse conditions, WPE-treated Chenopodium amaranticolor plants strongly inhibited TMV infection and significantly reduced TMV accumulation levels when compared to non-treated plants. Moreover, the induction of systemic resistance with significant increases in the transcriptional levels of the pathogenesis-related protein-1 (PR-1), chalcone synthase (CHS), and hydroxycinnamoyl-CoA quinate transferase (HQT) genes for treated plants were noticed at 3 and 5 days post-inoculation (dpi) for both assays. To the best of our knowledge, this is the first reported observation of the antiviral activity of H. tuberculatum extract against plant viral infections. Finally, the results obtained suggest that H. tuberculatum WPE can be considered a promising source of both antifungal and antiviral substances for practical use and for developing plant-derived compounds for the effective management of plant diseases.

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Section: Methodsmentioning

confidence: 99%

The Phytochemical, Antifungal, and First Report of the Antiviral Properties of Egyptian Haplophyllum tuberculatum Extract

Abdelkhalek

Salem

Hafez

et al. 2020

Biology

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“…Under insect-proof greenhouse conditions, tomato seeds were surface sterilized and cultivated in plastic pots (20 cm in diameter) filled with sterilized soil (clay: sand, 1:1). At 28th days post seedlings, tomato seedlings were transplanted into new pots, and one week later, two upper true leaves of each tomato plant were dusted with carborundum and mechanically inoculated with 1 mL of semi-purified TMV as previously described [43,44]. The experiment was carried out in five treatments, each treatment was comprised of five replicates, and each pot contained three tomato plants.…”

Section: Greenhouse Experimental Design and Antiviral Activity Assaymentioning

confidence: 99%

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

2020

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Globally, plant viral infection is one of the most difficult challenges of food security, where considerable losses in crop production occur. Nanoparticles are an effective control agent against numerous plant pathogens. However, there is limited knowledge concerning their effects against viral infection. In the present study, the green synthesis of zinc oxide nanoparticles (ZnO NPs) using aqueous leaf extract of Mentha spicata was achieved. X-ray diffraction patterns confirmed the crystalline nature of the prepared ZnO NPs. Dynamic light scattering and scanning electron microscopy analyses revealed that the resultant ZnO NPs were spherical in shape with a particle size ranged from 11 to 88 nm. Fourier transmission infrared spectroscopy detected different functional groups, capping and stability agents, and showed Zn-O bond within wavenumber of 487 cm−1. Under greenhouse conditions, the antiviral activity of biological synthesized ZnO NPs (100 µg/mL) against Tobacco mosaic virus (TMV) was evaluated. The double foliar application of the prepared ZnO NPs, 24 h before and 24 h after TMV-inoculation, was the most effective treatment that showed a 90.21% reduction of viral accumulation level and disease severity. Additionally, the transcriptional levels of PAL, PR-1 (salicylic acid marker gene), CHS, and POD genes were induced and up-regulated in all ZnO NPs treated plants. Notably, the results exhibited that aqueous extract of Mentha spicata was an effective reducing agent for the green synthesis of ZnO NPs, which showed significant antiviral activity. Finally, the detected protective and curative activity of ZnO NPs against TMV can encourage us to recommend its application for plant viral disease management. To our knowledge, this is the first study describing the antiviral activity of the green synthesized ZnO NPs.

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“…1 and Table 1 ). The amino acid sequences of N protein from different IYSV isolates have high similarity, and thus the IYSV N protein is relatively conserved [ 12 , 31 , 32 ]. With these antibodies, we developed the TAS-ELISA and MIA methods for detecting IYSV.…”

Section: Discussionmentioning

confidence: 99%

“…IYSV was firstly recorded in onion crops in Idaho, USA in 1989 [ 4 ] and then in the Netherlands in 1992, where it was characterized to be a new, distinct orthotospovirus from Iris hollandica [ 5 ]. Subsequently, IYSV was found to occur in [ 6 ] Brazil [ 7 ], Australia [ 8 ], Japan [ 9 ], Chile [ 10 ], Spain [ 11 ], Guatemala [ 12 ], Peru [ 13 ], India [ 14 ], and Egypt [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus

Yang

Song

et al. 2018

Virol J

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BackgroundIris yellow spot virus (IYSV) is an Orthotospovirus that infects most Allium species. Very few approaches for specific detection of IYSV from infected plants are available to date. We report the development of a high-sensitive Luminex xMAP-based microsphere immunoassay (MIA) for specific detection of IYSV.ResultsThe nucleocapsid (N) gene of IYSV was cloned and expressed in Escherichia coli to produce the His-tagged recombinant N protein. A panel of monoclonal antibodies (MAbs) against IYSV was generated by immunizing the mice with recombinant N protein. Five specific MAbs (16D9, 11C6, 7F4, 12C10, and 14H12) were identified and used for developing the Luminex xMAP-based MIA systems along with a polyclonal antibody against IYSV. Comparative analyses of their sensitivity and specificity in detecting IYSV from infected tobacco leaves identified 7F4 as the best-performed MAb in MIA. We then optimized the working conditions of Luminex xMAP-based MIA in specific detection of IYSV from infected tobacco leaves by using appropriate blocking buffer and proper concentration of biotin-labeled antibodies as well as the suitable ratio between the antibodies and the streptavidin R-phycoerythrin (SA-RPE). Under the optimized conditions the Luminex xMAP-based MIA was able to specifically detect IYSV with much higher sensitivity than conventional enzyme-linked immunosorbent assay (ELISA). Importantly, the Luminex xMAP-based MIA is time-saving and the whole procedure could be completed within 2.5 h.ConclusionsWe generated five specific MAbs against IYSV and developed the Luminex xMAP-based MIA method for specific detection of IYSV in plants. This assay provides a sensitive, high-specific, easy to perform and likely cost-effective approach for IYSV detection from infected plants, implicating potential broad usefulness of MIA in plant virus diagnosis.

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Occurrence of iris yellow spot virus from onion crops in Egypt

Cited by 27 publications

References 17 publications

The Phytochemical, Antifungal, and First Report of the Antiviral Properties of Egyptian Haplophyllum tuberculatum Extract

The Phytochemical, Antifungal, and First Report of the Antiviral Properties of Egyptian Haplophyllum tuberculatum Extract

Green Synthesized ZnO Nanoparticles Mediated by Mentha Spicata Extract Induce Plant Systemic Resistance against Tobacco Mosaic Virus

Development of a sensitive Luminex xMAP-based microsphere immunoassay for specific detection of Iris yellow spot virus

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