Occurrence of Listeria species in retail poultry meat and comparison of a cultural/immunoassay for their detection

Capita, Rosa; Alonso‐Calleja, Carlos; Moreno, B.; García-Fernández, M. C.

doi:10.1016/s0168-1605(00)00497-9

Cited by 70 publications

(47 citation statements)

References 36 publications

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“…Considering the results of Osaili et al (2011) andSoultos et al (2003) regarding Listeria spp., approximate values to our study were observed and they were very low in terms of L. monocytogenes while the rate of Listeria spp. found by Capita et al (2001) were very high in comparison with our results. The highly low rates of L. monocytogenes in the studies of Osaili et al (2011), Soultos et al (2003, Meyer et al (2012) and Capita et al (2001) in comparison with our study may be related to the microbial profile of poultry houses, cleanliness and hygienic conditions applied in the poultry slaughters, personal hygenie and low level of cross contamination.…”

Section: Resultssupporting

confidence: 54%

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Prevalence of Listeria monocytogenes in poultry meat

2015

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The objectives of this study were: i) to isolate Listeria spp. and Listeria monocytogenes in broiler wing meat samples, ii) to confirm the isolates by PCR, based on prs and hly A gene sequences, iii) to determine the seasonal and monthly distribution of the isolates. A total of 120 broiler wing meat samples (60 packaged pieces wrapped using strech film in styrofoam plates and 60 unpackaged pieces) bought from different markets in Hatay province were analysed. Listeria spp. was isolated from 57 (47.5%) out of 120 samples. Fifty-four, out of 57 Listeria spp. isolates were identified as L. monocytogenes. L. monocytogenes was isolated from the samples collected during the spring, winter, summer, and autumn at the levels of 26.6%, 40%, 53.3%, 60%, respectively. In this study, the isolation rates were found to be the highest in autumn, while the isolation rates were found to be the lowest in spring. As a consequence, high prevalence of Listeria spp. and L. monocytogenes in poultry wing meat samples may pose a risk for human health. We consider that with obeying the rules of good hygiene practices (GHP), good manufacturing practices (GMP) and HACCP can minimize the contamination with Listeria spp.Keywords: broiler wing meat; L. monocytogenes; Listeria spp.; hygienic quality.Practical Application: Taking hygienic measures in broiler flocks at farm level may minimize the contamination with Listeria.

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Section: Resultssupporting

confidence: 54%

“…Another researcher Soultos et al (2003) isolated Listeria spp. from 48% (38/80) of the broiler meat samples, and 14 (18%) of these samples were determined as L. monocytogenes whereas Capita et al (2001) found Listeria spp. in the 95% of the 100 broiler carcasses, and L. monocytogenes was identified from 15% of the samples.…”

Section: Resultsmentioning

confidence: 99%

Prevalence of Listeria monocytogenes in poultry meat

2015

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“…The growth rate of L. monocytogenes in SEL was superior to that in FB (47). In addition, other bacteria, Bacillus, Enterococcus, and Streptococcus spp., which showed poor or no growth in SEL (Table 1) can grow in FB (12).…”

Section: Discussionmentioning

confidence: 99%

SEL, a Selective Enrichment Broth for Simultaneous Growth of Salmonella enterica , Escherichia coli O157:H7, and Listeria monocytogenes

Kim

Bhunia

2008

Appl Environ Microbiol

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Multipathogen detection on a single-assay platform not only reduces the cost for testing but also provides data on the presence of pathogens in a single experiment. To achieve this detection, a multipathogen selective enrichment medium is essential to allow the concurrent growth of pathogens. SEL broth was formulated to allow the simultaneous growth of Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes. The results were compared to those obtained with the respective individual selective enrichment broths, RappaportVassiliadis (RV) for S. enterica, modified E. coli broth with 20 mg of novobiocin/liter for E. coli O157:H7, and Fraser broth for L. monocytogenes, and a currently used universal preenrichment broth (UPB). The growth of each pathogen in SEL inoculated at 10 1 or 10 3 CFU/ml was superior to that in the respective individual enrichment broth, except in the case of RV, in which Salmonella cells inoculated at both concentrations grew equally well. In mixed-culture experiments with cells of the three species present in equal concentrations or at a 1:10:1,000 ratio, the overall growth was proportional to the initial inoculation levels; however, the growth of L. monocytogenes was markedly suppressed when cells of this species were present at lower concentrations than those of the other two species. Further, SEL was able to resuscitate acid-and cold-stressed cells, and recovery was comparable to that in nonselective tryptic soy broth containing 6% yeast extract but superior to that in the respective individual selective broths. SEL promoted the growth of all three pathogens in a mixture in ready-to-eat salami and in turkey meat samples. Moreover, each pathogen was readily detected by a pathogen-specific immunochromatographic lateral-flow or multiplex PCR assay. Even though the growth of each pathogen in SEL was comparable to that in UPB, SEL inhibited greater numbers of nontarget organisms than did UPB. In summary, SEL was demonstrated to be a promising new multiplex selective enrichment broth for the detection of the three most prominent food-borne pathogens by antibody-or nucleic acid-based methods.

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“…Alternatively, immunoassays using monoclonal antibodies specific for Listeria species are available. A recent study of the "Listeria rapid test" showed a sensitivity of 100% and specificity of 86% for detecting Listeria species organisms on animal carcasses [49]. The disadvantage of this test is its inability to determine the species of Listeria, which is important because human disease is only caused by L. monocytogenes.…”

Section: Diagnosismentioning

confidence: 99%

“…Because L. monocytogenes organisms are ubiquitous in the environment, food contamination is common and seemingly inevitable. One recent study showed that 32% of chicken carcasses are contaminated with L. monocytogenes organisms, and the general population frequently consumes food items that are undercooked or unpasteurized [49]. The latter is an important issue, as our society enjoys many readymade foods, including hot dogs and deli meats.…”

Section: Preventionmentioning

confidence: 99%

Update on listeria monocytogenes infection

Crum

2002

Curr Gastroenterol Rep

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Listeria monocytogenes is a gram-positive bacillus that causes meningitis, encephalitis, bacteremia, and febrile gastroenteritis. Most disease occurs in immunosuppressed individuals. Recent seroepidemiologic studies show that the infection is foodborne. Due to the increasing number of immunosuppressed individuals at risk for listeriosis, as well as the persistence of substantial foodborne outbreaks, L. monocytogenes has gained worldwide attention as an important pathogen. Heightened surveillance and quality control by the food industry have been instituted, leading to a reduction in the number of cases and deaths from this infection in the past decade. However, due to the ubiquity of the organism in the environment, outbreaks and sporadic disease continue to occur. The standard therapy for listeriosis is a combination of ampicillin and gentamicin or, for patients who are intolerant of b-lactam agents, trimethoprim-sulfamethazole. Despite the availability of therapy, the mortality rate remains high in those with T-cell immunodeficiencies.

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Occurrence of Listeria species in retail poultry meat and comparison of a cultural/immunoassay for their detection

Cited by 70 publications

References 36 publications

Prevalence of Listeria monocytogenes in poultry meat

Prevalence of Listeria monocytogenes in poultry meat

SEL, a Selective Enrichment Broth for Simultaneous Growth of Salmonella enterica , Escherichia coli O157:H7, and Listeria monocytogenes

Update on listeria monocytogenes infection

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