Occurrence of motile Aeromonas in municipal drinking water and distribution of genes encoding virulence factors

Pablos, M; Rodríguez-Calleja, José M.; Santos, Jesús A.; Otero, Andrés; García-López, María-Luisa

doi:10.1016/j.ijfoodmicro.2009.08.020

Cited by 68 publications

(54 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ottaviani et al (2011) also reported that 96.4% of the food-borne Aeromonas isolates were positive for at least one toxin genes. However, Pablos et al (2009) reported that all the Aeromonas isolates tested in their study were positive for alt gene, which is at par with our findings. The overall prevalence of the alt, act/hlyA/aer and ast genes was recorded to be 89.8%, 72.9% and 20%, respectively.…”

Section: Discussionsupporting

confidence: 82%

“…The overall prevalence of the alt, act/hlyA/aer and ast genes was recorded to be 89.8%, 72.9% and 20%, respectively. In disagreement with some of the earlier reports (Sen and Rodger, 2004;Balsalobre et al, 2009) but in accordance with the others (Pablos et al, 2009;Bin Kingombe et al, 2010;Ottaviani et al, 2011;Pinto et al, 2012), the alt gene was found to be dominant among the MPCR positive isolates of Aeromonas spp. Balsalobre et al (2009) reported that 70.7%, 97.6% and 26.8% of the A. hydrophila strains tested were positive for act/hlyA/aer, ast and alt genes, respectively, and 2.4% and 36.6% of the A. jandaei strains were positive for act/hlyA/aer complex and alt genes.…”

Section: Discussioncontrasting

confidence: 66%

“…is multifactorial and incompletely understood (Pablos et al, 2009). A number of virulence factors such as extracellular enzymes, siderophores, cytotoxic and cytotonic enterotoxins, haemolysins, endotoxins and cell associated factors such as S-layers, pili, polar and lateral flagella, outer membrane proteins and plasmids were associated with pathogenicity of Aeromonas spp.…”

Section: Introductionmentioning

confidence: 99%

“…A number of virulence factors such as extracellular enzymes, siderophores, cytotoxic and cytotonic enterotoxins, haemolysins, endotoxins and cell associated factors such as S-layers, pili, polar and lateral flagella, outer membrane proteins and plasmids were associated with pathogenicity of Aeromonas spp. (Snowden et al, 2006;Pablos et al, 2009). Exotoxins are major virulence factors of Aeromonas spp.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Hussain¹,

Jeyasekaran²,

Shakila³

et al. 2013

AJAFST

View full text Add to dashboard Cite

A total of 103 representative colonies from 53 healthy and diseased food fish samples were tested for the incidence of Aeromonas spp. Of which 57% of colonies were positive for Aeromonas spp. by the multiplex PCR (MPCR). On an average, the prevalence was found to be 66% with highest incidence in diseased fishes (76%). About 48% of healthy fish samples were observed to contain Aeromonas. The biomoleculoar identification revealed that the selected virulent hemolytic and enterotoxigenic genes appeared in four different patterns viz. alt; act//hlyA/aer; alt and act//hlyA/aer; ast, alt and act//hlyA/aer, among the MPCR positive isolates of Aeromonas spp. The overall prevalence of the toxin genes alt, act/hlyA/aer and ast among the MPCR positive Aeromonas isolates was found to be 89.8%, 72.9% and 20%, respectively. The biochemical confirmation of the MPCR positive Aeromonas isolates revealed different species in the tested samples belonging to A. hydrophila, A. sobria, A. caviae, A. jandaei, A. veronii, A. schubertii and A. trota and the dominant species was found to be A. hydrophila (48%).

show abstract

Section: Discussionsupporting

confidence: 82%

Section: Discussioncontrasting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Hussain¹,

Jeyasekaran²,

Shakila³

et al. 2013

AJAFST

View full text Add to dashboard Cite

show abstract

“…Virulence of Aeromonas spp. is multifactorial and incompletely understood (Pablos et al 2009). A number of virulence factors derived from A. hydrophila and A. sobria have been examined in an effort to explain the pathogenesis of infections due to these organisms.…”

Section: Introductionmentioning

confidence: 99%

Detection of hemolytic strains of Aeromonas hydrophila and A. sobria along with other Aeromonas spp. from fish and fishery products by multiplex PCR

et al. 2013

View full text Add to dashboard Cite

Hemolytic strains of Aeromonas spp. from fish and fishery products were detected by multiplex PCR. The selected primers for the amplification of segments of ahh1, asa1 and 16S rRNA gene yielded products with the size of 130 bp, 249 bp and 356 bp, respectively. This assay was found to be highly sensitive, as it could detect 7 and 9 cells of Aeromonas hydrophila and A. sobria with a detection limit of 1 pg of pure genomic DNA. The assay, when screened for 73 commercial fish and fishery product samples consisting of freshwater, marine fish and shellfish, showed 56 % positive for Aeromonas spp., 16 % for Aeromonas hydrophila and 13 % for A . sobria. This assay provides specific and reliable results and can be a powerful tool for the simultaneous detection of hemolytic strains of A . hydrophila A . sobria and other Aeromonas spp. from fish and fishery products.

show abstract

Aeromonas*

Lamy

2023

ClinMicroNow

View full text Add to dashboard Cite

Members of the genus Aeromonas are Gram‐negative facultative anaerobes that are straight, coccobacillary to bacillary cells with rounded ends. Aeromonas species are environmental opportunistic pathogens, involved in numerous types of diseases, including gastroenteritis and infections at a variety of extraintestinal sites, of which blood and wounds are the most common. Most clinical infections with aeromonads are related to an exposure to some type of aquatic source, whether the clinical specimen is feces or extraintestinal, and to a lesser extent to the ingestion of foods. Aeromonads survive well in specimens, and any of the widely used transport media are acceptable for transport, with Cary‐Blair generally considered to be the best for feces transportation. Whole‐genome sequencing is the reference method to identify aeromonads or to describe a novel species. Strains show a propensity to acquire resistance genes and to disseminate them to other bacteria. Antimicrobial susceptibility testing of local isolates is necessary because of emerging resistance.

show abstract

Occurrence of motile Aeromonas in municipal drinking water and distribution of genes encoding virulence factors

Cited by 68 publications

References 36 publications

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Prevalence of Hemolytic and Enterotoxigenic Aeromonas Spp. in Healthy and Diseased Freshwater Food Fishes as Assessed by Multiplex PCR

Detection of hemolytic strains of Aeromonas hydrophila and A. sobria along with other Aeromonas spp. from fish and fishery products by multiplex PCR

Aeromonas*

Contact Info

Product

Resources

About