Occurrence of pathogenic Escherichia coli in commercially available fresh vegetable products in Korea

Kim, Hyun Jung; Koo, Minseon; Jeong, Aram; Baek, Seung-Youb; Cho, Joon-Il; Lee, Soonho; Hwang, Inchan

doi:10.1007/s13765-014-4073-5

Cited by 10 publications

(5 citation statements)

References 19 publications

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“…The total live bacteria content of eggs given at private and governmental hospitals varied significantly (P≤0.05) at all stages of the food chain, with a probable decrease in all samples during the processing step, where the egg was heated to boil. This is in line with the findings of (Kim et al, 2014), who found that the total bacterial count was reduced from 3.7 to 0.9 log10 CFU/g during and after cooking.…”

Section: Resultssupporting

confidence: 92%

The Impact of Food Services Protocol on the Quality and Safety of Egg Served by Hospitals in Khartoum State, Sudan

Farahat

Mustafa²,

Nada³

2022

Alexandria Journal of Food Science and Technology

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This work purpose was to assess the safety and quality of hospital meals and services. The egg was taken in several phases of the hospital food chain (raw food, storage, preparation, processing, and display) to highlight the cause of nutrient, safety, and quality changes in patient meals in Khartoum State. Chemical composition (moisture content, ash, protein and fat percents),total titrable acidity and pH value) and microbial loads (total viable count, yeast and mould, total Coliform bacteria, Salmonella and Staphylococcus spp.) were measured at all stages. The moisture content of eggs showed a significant (P≤0.05) slight decrease in private hospitals and a potential increase in government hospitals, while fat content showed a slight non-significant decrease in all samples, except for the sample taken from (D) governmental hospital, which showed a significant potential increase at the end-stage (processing and display stages), There were no significant differences (P≤0.05) in ash content, titratable acidity and pH served at all hospitals in all chain stages. Total bacterial count served at hospitals, all samples showed a significant (P≤0.05) decrease during food chain stages and total yeasts and moulds content served at privatized hospitals.

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Section: Resultssupporting

confidence: 92%

The Impact of Food Services Protocol on the Quality and Safety of Egg Served by Hospitals in Khartoum State, Sudan

Farahat

Mustafa²,

Nada³

2022

Alexandria Journal of Food Science and Technology

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“…This could lead to harm, especially when the people’s awareness on this matter is very low. Several publications reported the presence of pathogenic E. coli in fresh vegetables and fruits [6, 7]. Therefore, it is necessary to conduct a research on the detection and level of contamination of pathogenic E. coli from salad vegetables and fruits sold in traditional markets and grocery stores in Jakarta.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of pathogenic Escherichia coli from salad vegetable and fruits sold in Jakarta

2019

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Objective Escherichia coli is a normal inhabitant of mammalian’s gut, but some strains acquired virulence factor and became pathogenic. Enterotoxigenic E. coli (ETEC), enterohemorrhagic E. coli (EHEC), enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), and enteroaggregative E. coli (EAEC) are among pathogenic strains of E. coli. Vegetables and fruits could be sources of transmission. Samples were collected and subjected to three-tubes Most Probable Number (MPN) analysis followed by Multiplex PCR. Six sets of primer encoding virulence genes were used: stx , ipah , aggr , eae , elt and est . Results From this study we found, the highest maximum number for the MPN result reached > 1100 MPN/mL and the lowest is 3 MPN/mL. From first multiplex PCR showed 65 salad vegetable samples, 7.69% were positive and from the 63 fruit samples, 11.11% were positive. From second multiplex PCR for 76 isolates, 55 (72.37%) isolates were aggR positive (EAEC), 12 (15.79%) isolates were eae positive (EPEC), and 9 (11.84%) were elt positive (ETEC). Antimicrobial resistance assay showed that 83.33% of the isolates were multi resistant. Resistances are observed to 10 μg Ampicillin (22.22%), 5 μg Ciprofloxacin (11.11%), 10 μg Gentamycin (33.33%), 30 μg Kanamycin (38.89%), 10 μg Streptomycin (55.56%), 5 μg Trimethoprim (16.67%), and 300 U Polymyxin B (61.11%).

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“…Although E. coli is not a natural inhabitant of the soil or rhizosphere, it has been reported to grow in different soil types ranging from tropical to temperate regions ( Oliver et al, 2006 ; Semenov et al, 2008 ; Nautiyal, Rehman & Chauhan, 2010 ). Some studies report the presence of E. coli in salads (lettuce), vegetables and fruits and furthermore some strains have shown multidrug resistance capability ( Solomon, Yaron & Matthews, 2002 ; Buchholz et al, 2011 ; Chekabab et al, 2013 ; Kim et al, 2014 ; Waturangi, Hudiono & Aliwarga, 2019 ). Recently, E. coli has been reported to be endophytic in plant leaves ( Tharek et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of colonization and mutualistic endophytic symbiosis of Escherichia coli with tomato and Bermuda grass seedlings

Verma

Chen

White

2022

PeerJ

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Escherichia coli is generally considered a bacterium associated with animal microbiomes. However, we present evidence that E. coli may also mutualistically colonize roots of plant species, even to the extent that it may become endophytic in plants. In this study we used GFP tagged E. coli to observe its colonization and effects on tomato (Solanum lycopersicum) and Bermuda grass (Cynodon dactylon) seedling development and growth. Inoculation with the bacterium significantly improved root development of both seedlings tested. Treatment also increased the photosynthetic pigments in Bermuda grass seedlings. However, effects on shoot length in both seedlings were not significant. This bacterium was found to produce indole acetic acid (IAA) up to 8.68 ± 0.43 µg ml−1 in the broth medium amended with tryptophan. Effects on seedling root growth could, in part, be explained by IAA production. Bacteria successfully colonized the root surfaces and interiors of both seedlings. Tagged bacteria expressing the GFP were observed in the vascular tissues of Bermuda grass seedling roots. Seedlings with bacteria showed greater survival and were healthier than seedlings without bacteria, indicating that E. coli set up a successful mutualistic symbiosis with seedlings. E. coli is not commonly considered to be a plant endophyte but is more generally considered to be a crop contaminant. In this study we show that E. coli may also be an endophyte in plant tissues.

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Occurrence of pathogenic Escherichia coli in commercially available fresh vegetable products in Korea

Cited by 10 publications

References 19 publications

The Impact of Food Services Protocol on the Quality and Safety of Egg Served by Hospitals in Khartoum State, Sudan

The Impact of Food Services Protocol on the Quality and Safety of Egg Served by Hospitals in Khartoum State, Sudan

Prevalence of pathogenic Escherichia coli from salad vegetable and fruits sold in Jakarta

Evaluation of colonization and mutualistic endophytic symbiosis of Escherichia coli with tomato and Bermuda grass seedlings

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