Occurrence of spermic diploid and aspermic triploid forms of Fasciola in Vietnam and their molecular characterization based on nuclear and mitochondrial DNA

Itagaki, Tadashi; Sakaguchi, Keisuke; Terasaki, Kunio; Sasaki, Osamu; Yoshihara, Shinobu; Dung, Truong Van

doi:10.1016/j.parint.2008.11.003

Cited by 87 publications

(83 citation statements)

References 24 publications

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“…In particular, we aimed to determine a cut off with high sensitivity and specificity. We used the Itagaki et al (16) dataset as a test set to assess the external validity of our results. We applied the optimal cut off derived from our data to Itagaki et al (16) dataset.…”

Section: Discussionmentioning

confidence: 99%

“…We used the Itagaki et al (16) dataset as a test set to assess the external validity of our results. We applied the optimal cut off derived from our data to Itagaki et al (16) dataset. Results are presented in terms of sensitivity and specificity.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, the L/W ratio was proved as a sensitive and specific index for discriminating F. hepatica and F. gigantica isolates. We used the Itagaki et al (16) dataset based on molecular tools to test our morphometric model in a set of Fasciola isolates that were positively identified by rDNA as well as mitochondrial sequencing. The ROC curve analysis was conducted for the first time to find a discrimination threshold with the highest sensitivity and specificity for distinguishing Fasciola species.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, we postulated that all the isolates with an L/W value >3.55 are F. gigantica with 100% sensitivity and specificity. When we applied this cut off to the Itagaki et al (16) dataset, sensitivity and specificity values were 90% and 85%, respectively. As shown in Table 1, a significant difference in the body length and width as well as L/W was recorded between the two species of Fasciola (p<0.05).…”

Section: Morphometric Findingsmentioning

confidence: 99%

“…Additionally, the hybridization phenomenon occurs where both species co-exist. Fasciola forms intermediate between F. hepatica and F. gigantica have been reported from Asian countries, including Korea (11), Japan (12), Iran (13), China (14), and Vietnam (15,16) as well as African countries including Egypt (17,18).…”

Section: Introductionmentioning

confidence: 99%

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Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran

Akhlaghi¹,

Mohammadi²,

Ziaali³

et al. 2018

TurkiyeParazitolDerg

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Objective: The purpose of the present study was morphometric and molecular characterization of Fasciola isolates from ruminants in Iran. Methods: Flukes were collected from the livers of 54 naturally infected sheep and cattle. The proportion of body length to width (L/W) of each fresh fluke was measured using a digital caliper. We employed receiver operating characteristic (ROC) curve analysis to explore the reliability of L/W for differentiating the two species. Polymerase chain reaction (PCR)-sequencing was performed on ribosomal Internal Transcribed Spacers (ITS) and mitochondrial cytochrome c oxidase subunit 1 (cox1) genes. The sequences were then analyzed and phylogenetic relationships were investigated. Result: Forty-eight out of 54 isolates (88.9%) were identified as F. hepatica and four isolates (7.4%) as F. gigantica. All the sheep isolates were F. hepatica, while 4 out of 10 cattle were infected with F. gigantica. The morphometric study revealed an L/W ratio of 1.2 to 6.5 in Fasciola isolates with significantly higher L/W ratio in F. gigantica (p<0.00). According to the ROC curve analysis, the L/W value of 3.55 was regarded as the critical value to discriminate between the two species. Conclusions: Findings of the present study indicate the presence of both Fasciola species in southeastern Iran. The phylogenetic analysis revealed two different clades representing F. hepatica and F. gigantica. The two isolates in this study were described as Fasciola sp. The mitochondrial DNA of these isolates were similar to F. hepatica, while their ITS fragments were identical to F. gigantica.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Morphometric Findingsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphometric and Molecular Study of Fasciola Isolates from Ruminants in Iran

Akhlaghi¹,

Mohammadi²,

Ziaali³

et al. 2018

TurkiyeParazitolDerg

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Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Sharbatkhori

Nasibi

Mohammadi

et al. 2023

Veterinary Medicine & Sci

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Background: Fascioliasis, caused by the liver flukes Fasciola hepatica and Fasciola gigantica, is a global zoonotic helminthic disease. The livestock and human are the final hosts of the parasites. Northern Iran is an important endemic region for fascioliasis. Few studies have been conducted on the characterization of Fasciola isolates from eastern regions of the Caspian littoral of the country. Objective:The aim of the present study was to identify F. hepatica, F. gigantica and intermediate/hybrid forms of Fasciola isolates from livestock in Golestan province, northern Iran, using morphometric and molecular tools.Methods: Livestock livers naturally infected with Fasciola spp. were collected from Golestan slaughterhouse during 2019-2020. The worms were morphometrically studied using a calibrated stereomicroscope. Genomic DNA was extracted from all samples, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on internal transcribed spacer (ITS1) region using Rsa1 restriction enzyme. All the isolates were then analysed by multiplex PCR on Pepck region.Results: A total of 110 Fasciola isolates were collected from the infected livers, including 94 sheep, 12 cattle and 4 goats. Morphometric analysis of 61 adult Fasciola isolates indicated that, 44 and 17 isolates belonged to F. hepatica and F. gigantica, respectively.Eighty-one and 29 isolates belonged to F. hepatica and F. gigantica using ITS1-RFLP, respectively. However, Pepck Multiplex PCR indicated 72 F. hepatica, 26 F. gigantica and 12 intermediate/hybrid forms. All 12 hybrid isolates were found in sheep host. Two isolates were identified as F. gigantica using morphometry and F. hepatica using both molecular methods. Conclusion:The present study confirmed the existence of both F. hepatica and F. gigantica species and reported the first molecular evidence of hybrid Fasciola isolates in ruminants of Golestan province.

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