Occurrence of the mucinous differentiation antigen CA125 in genital tract and conductive airway epithelia of diverse mammalian species (rabbit, dog, monkey)

Nouwen, Etienne J.; Dauwe, Simonne; Broe, Marc E. De

doi:10.1111/j.1432-0436.1990.tb00473.x

Cited by 19 publications

(12 citation statements)

References 19 publications

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“…In contrast, MUC16 was localized to the cytoplasm of the goblet cells and the mucous cells in the submucosal glands. This finding is supported by other studies where the mucin has been identified in airway submucosal gland cells (Nouwen, Dauwe, & De Broe, 1990), lacrimal glands (Jäger et al, 2007) and conjunctival goblet cells (Argueso, Spurr-Michaud, Russo, Tisdale, & Gipson, 2003). In conjunctiva, MUC16 was seen in mucin packages in the goblet cells suggesting that the molecule may be co-localized in the secretory granulae with large oligomeric mucins.…”

Section: Discussionsupporting

confidence: 85%

MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells

Davies

Kirkham

Svitacheva

et al. 2007

The International Journal of Biochemistry & Cell Biology

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Section: Discussionsupporting

confidence: 85%

MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells

Davies

Kirkham

Svitacheva

et al. 2007

The International Journal of Biochemistry & Cell Biology

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“…2 B). In line with these results, the ortholog of Muc16 from human, monkey, rabbit and dog, called CA125 , was found to be expressed in the adult mesothelium [Nouwen et al, 1990], a structure derived from the coelomic epithelium. Of note, human CA125 is commonly used as a marker for diagnosis and following of epithelial ovarian carcinomas [Bast et al, 2005].…”

Section: Coelomic Epitheliumsupporting

confidence: 54%

WT1-Mediated Gene Regulation in Early Urogenital Ridge Development

Klattig

Sierig²,

Kruspe³

et al. 2007

Sex Dev

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The Wilms tumor protein WT1 is involved in the development of several organs, including the gonads. WT1 mutations in humans lead to syndromes associated with impaired sexual development and Wt1 knockout mice show regression of gonad anlagen. As a transcription factor, WT1 fulfills its function by regulating a set of target genes. With respect to gonad development only few in vivo WT1 targets, e.g. steroidogenic factor 1 (SF1) have been identified so far. To get a comprehensive view of WT1 targets in the gonad, we compared gene expression in urogenital ridges of wild-type and Wt1^–/– embryos. We found almost 150 genes differentially expressed higher than factor three, using microarray analysis. To confirm these results we performed quantitative real-time RT-PCR for many genes and observed a high degree of concordance between microarray and real-time RT-PCR results. Employing in situ hybridization we found ‘WT1 activated genes’ to be expressed in gonads, mesonephroi and coelomic epithelium – those parts of the urogenital ridge with Wt1 expression. Interestingly, many of the differentially expressed genes are known to show sex-specific expression at later time-points. These results provide a basis for investigation of developmental pathways in the urogenital ridge downstream of WT1 and for identification of new candidate genes involved in early urogenital ridge development. For example we provide a first potential target of WT1 in the coelomic epithelium – Muc16, and a gene regulated by the WT1 target SF1 – Gata4.

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“…CA125 is the most extensively studied biomarker for possible use in the early detection of ovarian carcinoma [23][24][25][26][27][28]. However, although CA125 is conserved in some mammals [29,30] its study is hampered by its lack of conservation in rodents. The absence of CA125 in rodents may be due to ovarian biological differences and thus it is difficult to extrapolate from mouse to human.…”

Section: Introductionmentioning

confidence: 99%

Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery

et al. 2007

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Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell attachment. We also showed that this assay supplies a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. We finally demonstrated that a mesothelin chimeric protein and anti-CA125 antibodies block CA125/mesothelin-dependent cell attachment.

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Occurrence of the mucinous differentiation antigen CA125 in genital tract and conductive airway epithelia of diverse mammalian species (rabbit, dog, monkey)

Cited by 19 publications

References 19 publications

MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells

MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells

WT1-Mediated Gene Regulation in Early Urogenital Ridge Development

Development of a CA125-mesothelin cell adhesion assay as a screening tool for biologics discovery

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