Occurrence of virulence genes among Vibrio cholerae and Vibrio parahaemolyticus strains from treated wastewaters

Khouadja, Sadok; Suffredini, Elisabetta; Baccouche, Besma; Croci, Luciana; Bakhrouf, Amina

doi:10.1007/s10661-014-3900-9

Cited by 16 publications

(12 citation statements)

References 29 publications

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“…For instance, two phages VfO4K68 and VfO3K6 that carry the zona occludens toxin (Zot) and the accessory cholera enterotoxin (Ace), have been isolated from V. parahaemolyticus (4, 5). Zot and Ace are particularly common among vibriophages isolated from human pathogens, such as V. cholerae and V. parahaemolyticus (6-8) but are also present in prophage-like elements of non-human pathogens such as V. coralliilyticus (9) and V. anguillarum (10), suggesting frequent HGT among different vibrio species (11).…”

Section: Introductionmentioning

confidence: 99%

Closely relatedVibrio alginolyticusstrains encode an identical repertoire of prophages and filamentous phages

Chibani

Hertel

Hoppert

et al. 2019

Preprint

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Filamentous vibriophages represent a massive repertoire of virulence factors which can be transferred across species boundaries, leading to the emergence of deadly pathogens. All filamentous vibriophages that were characterized until today were isolated from human pathogens. Considering frequent horizontal gene transfer among vibrios, we predict that other environmental isolates, including non-human pathogens also carry filamentous phages, of which some may encode virulence factors.The aim of this study was to characterize the phage repertoire, consisting of prophages and filamentous phages, of a marine pathogen, Vibrio alginolyticus. To do so, we sequenced eight different V. alginolyticus strains, isolated from different pipefish and characterised their phage repertoire using a combination of morphological analyses and comparative genomics.We were able to identify a total of five novel phage regions (three different Caudovirales and two different Inoviridae), whereby only those two loci predicted to correspond to filamentous phages (family Inoviridae) represent actively replicating phages. Unique for this study was that all eight host strains, which were isolated from different eukaryotic hosts have identical bacteriophages, suggesting a clonal expansion of this strain after the phages had been acquired by a common ancestor. We further found that co-occurrence of two different filamentous phages leads to within-host competition resulting in reduced phage replication by one of the two phages. One of the two filamentous phages encoded two virulence genes (Ace and Zot), homologous to those encoded on the V. cholerae phage CTXΦ. The coverage of these zot-encoding phages correlated positively with virulence (measured in controlled infection experiments on the eukaryotic host), suggesting that this phages is an important virulence determinant.Impact statementMany bacteria of the genus Vibrio, such as V. cholerae or V. parahaemolyticus impose a strong threat to human health. Often, small viruses, known as filamentous phages encode virulence genes. Upon infecting a bacterial cell, these phages can transform a previously harmless bacterium into a deadly pathogen. While filamentous phages and their virulence factors are well-characterized for human pathogenic vibrios, filamentous phages of marine vibrios, pathogenic for a wide range of marine organisms, are predicted to carry virulence factors, but have so far not been characterized in depth. Using whole genome sequencing and comparative genomics of phages isolated from a marine fish pathogen V. alginolyticus, we show that also environmental strains harbour filamentous phages that carry virulence genes. These phages were most likely acquired from other vibrios by a process known as horizontal gene transfer. We found that these phages are identical across eight different pathogenic V. alginolyticus strains, suggesting that they have been acquired by a common ancestor before a clonal expansion of this ecotype took place. The phages characterized in this study have not been described before and are unique for the Kiel V. alginolyticus ecotype.Data SummaryThe GenBank accession numbers for all genomic sequence data analysed in the present study can be found in Table S1.All phage regions identified by PHASTER analysis of each chromosome and the respective coverage of active phage loci are listed in Table S2.GenBank files were deposited at NCBI for the two actively replicating filamentous phages VALGΦ6 (Accession number: MN719123) and VALGΦ8 (Accession number: MN690600)The virulence data from the infection experiments have been deposited at PANGAEA: Accession number will be provided upon acceptance of the manuscript.Data statementAll supporting data have been provided within the article or through supplementary data files. Four supplementary tables and six supplementary figures are available with the online version of this article.

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Section: Introductionmentioning

confidence: 99%

Closely relatedVibrio alginolyticusstrains encode an identical repertoire of prophages and filamentous phages

Chibani

Hertel

Hoppert

et al. 2019

Preprint

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“…SH15-2 was isolated from mallard in Shenzhen. Ace and zot leaded cell membrane permeability to change of human small intestine, human diarrhea [25]. The same cholera toxin was integrated to nonmigratory birds VP through VfO3K6 and VfO4K68 in Mexico [26].…”

Section: Discussionmentioning

confidence: 98%

Virulence gene, antimicrobial resistance and phylogenetic characterization of Vibrio parahaemolyticus in migratory birds, Guangdong

Zheng

Chen²,

Guo

et al. 2020

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Background Vibrio parahaemolyticus is an intestinal pathogen that is transmitted by contaminated aquatic products.Migratory birds ate foods infected with Vibrio parahaemolyticus, excreting feces on other foods. Other organisms ate contaminated food again, leading to the spread of strains. It were reported previously that Vibrio parahaemolyticus was sensitive to most antibiotics except penicillin such as ampicillin and amoxicillin. However, due to the frequent used of antibiotics in recent years, Vibrio parahaemolyticus developed resistance to antibiotics. The recommended antibiotics for Vibrio parahaemolyticus treatment were tetracycline, β-lactam, quinolone and sulfonamide. In the storage migratory bird, Vibrio parahaemolyticus could complete the accumulation of drug resistance, resulting in the generation of drug resistance genes, or the horizontal drug resistance genes transfer from other bacteria, resulting obtain drug resistance genes. Results In this study, 122 isolates of Vibrio parahaemolyticus were obtained for further pathogenicity identification, antibacterial susceptibility testing of 18 antimicrobial compounds and genetic characterization based on the whole genome sequencing. There is no pandemic serotype isolated between migratory birds, but 37 strains with cholera toxin. Five strains had col3M plasmid-moderately resistant drug-resistant strains of quinolone mediated by the qnrD1 gene. For the first time in China, Vibrio parahaemolyticus with col3M plasmid-mediated qnrD1 gene was isolated from migratory birds. Conclusion According to the mutltilocus sequence typing analysis, authors found the bacterial genetic diversity is higher, the clones are less and there is regional aggregation in Shenzhen. And mallards are more susceptible to Vibrio parahaemolyticus.

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“…Abundance of Fusobacterium and Cetobacterium in birds NO.18 and NO.19 in intestine was little, and it was not the dominant genus of birds NO.18 and NO. 19. We believed that other phylum have begun to breed, especially Proteobacteia, resulting in the relative abundance of Fusobacteria reduced.…”

Section: Discussionmentioning

confidence: 99%

“…Abundance of Vibrio in lungs was higher than intestines and other organs. Vibrio infection was usually caused by eating food contaminated with Vibrio, which changed the permeability of the small intestinal epithelial cells, resulting in abnormal Na / K + pumps and diarrhea [19,20].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing

Zheng

Zhu

Guan

et al. 2020

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Background: In August 2018, a large number of migratory birds died in Chifeng, Neimeng. We were entrusted by local animal disease prevention and control center to collect the migratory bird epidemic materials and their living environmental water, and in 2019, we collect the local migratory bird stool and their living environmental water again. The bacterial communities in migratory bird epidemic materials, water and aquatic plants are profifiled by high-throughput sequencing of the V3–V4 hypervariable region of 16S rDNA gene.Results: We found that the dominant phylum between migratory bird epidemic materials, water and aquatic plant were Proteobacteia, Bacteroidetes, Firmicutes, Fusobacteria and Verrucomicrobia in 2018. One year later, we found that the dominant phylum between migratory bird stool, water and aquatic plant were Proteobacteia, Firmicutes, Cyanobacteria, Bacteroidetes. The relative abundance among bacterial phylum notably differed between two years. The relative abundance of Fusobacteria and Verrucomicrobia were higher in samples in 2018, while that of Cyanobacteria was higher in water, 2019. The relative abundance of Fusobacteria in migratory bird epidemic materials began to decline in the later period over time. At genus level, the relative abundance of Vibrio, Clostridium and other patnogenic bacteria decreased markedly and disappeared in 2019. The salt content and pH show a downward trend.Conclusions: Differences in diet and geographical location can lead to diversification in migratory bird intestinal flora. It is necessary to pay attention to diversification in intestinal flora of migratory birds, especially the abundance of vibrio in intestine. The overall structure of intestinal flora and relative abundance changes of various species are showed intuitively by 16S rDNA amplicon. But comparing to PCR which using specific primers, the accuracy and sensitivity are relatively poor. In the case of purposeful detection, it can be used in combination with both 16S rDNA amplicon and PCR which using specific primers. They are more accurately, because of supplement each other.

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Occurrence of virulence genes among Vibrio cholerae and Vibrio parahaemolyticus strains from treated wastewaters

Cited by 16 publications

References 29 publications

Closely relatedVibrio alginolyticusstrains encode an identical repertoire of prophages and filamentous phages

Closely relatedVibrio alginolyticusstrains encode an identical repertoire of prophages and filamentous phages

Virulence gene, antimicrobial resistance and phylogenetic characterization of Vibrio parahaemolyticus in migratory birds, Guangdong

Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing

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