Oceanobacillus indicireducens sp. nov., a facultative alkaliphile that reduces an indigo dye

Abstract

An indigo-reducing facultatively alkaliphilic and halophilic strain, designated strain A21 T , was isolated from a fermented Polygonum indigo (Polygonum tinctorium Lour.) liquor sample aged for 4 days prepared in a laboratory. 16S rRNA gene sequence phylogeny suggested that strain A21 T was a member of the genus Oceanobacillus with the closest relative being the type strain of Oceanobacillus chironomi (similarity: 96.0 %). The cells of the isolate stained Gram-positive and were facultatively anaerobic straight rods that were motile by peritrichous flagella. The strain grew between 18 and 48 6C with optimum growth at 39 6C. It grew in the pH range of 7-12. It hydrolysed casein, gelatin and Tween 20 but not Tweens 40, 60 and 80, starch or DNA. No isoprenoid quinone was detected and the DNA G+C content was 39.7 mol%. The whole-cell fatty acid profile mainly consisted of iso-C 15 : 0 , anteiso-C 15 : 0 and C 16 : 0 . DNA-DNA hybridization experiments with O. chironomi revealed 13 % relatedness. Owing to the differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analyses based on 16S rRNA gene sequences and DNA-DNA relatedness data from reported Oceanobacillus species, the isolate merits classification as a representative of a novel species, for which the name Oceanobacillus indicireducens sp. nov. is proposed. The type strain is A21 T (5JCM 17251 T 5NCIMB 14685 T ).The description of the genus Oceanobacillus is also emended.

Sinibacillus soli gen. nov., sp. nov., a moderately thermotolerant member of the family Bacillaceae

“…Although similar to strains GD05 T and GD051 in that the predominant fatty acid was anteiso-C 15 : 0 , V. pantothenticus DSM 26 T had much larger proportions of anteiso-C 15 : 0 (49.7 %) and anteiso-C 17 : 0 (27.5 %). The fatty acid profile of Oceanobacillus iheyensis in this study was consistent with that described by Kim et al (2007), but was different from that reported by Hirota et al (2013) who detected anteiso-C 15 : 0 as the predominant fatty acid, which suggested that the fatty acid profile of Oceanobacillus iheyensis varies with culture conditions. In this study, the fatty acids of the new isolates and Oceanobacillus iheyensis were determined using cells cultured under the same conditions, so the comparison was credible.…”

Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid

“…Recently, a novel strain of Oceanobacillus indicireducens, which does not possess menaquinone, has been reported (Hirota et al, 2013).…”

Fermentibacillus polygoni gen. nov., sp. nov., an alkaliphile that reduces indigo dye

“…We have also isolated indigo-reducing bacteria belonging to the genus Amphibacillus, namely Amphibacillus indicireducens and Amphibacillus iburiensis (Hirota et al, 2013a, c). Furthermore, we have isolated Oceanobacillus indicireducens as an indigo-reducing bacterium (Hirota et al, 2013b). Surprisingly, although strains belonging to this genus possess isoprenoid quinone, this bacterium does not possess any isoprenoid quinone.…”