Ocena związku między omentyną-1 a markerami metabolizmu kostnego i cytokinami systemu RANKL/RANK/OPG u dziewcząt z jadłowstrętem psychicznym

Abstract

Introduction: Omentin-1, secreted by visceral adipose tissue, has been indicated in the regulation of bone metabolism in girls with anorexia nervosa (AN). The aim of the study was to evaluate the relationship between omentin-1 and bone metabolism in girls with AN as well as the potential involvement of OPG and RANKL in this relationship. Material and methods: Serum omentin-1, OC, CTx, OPG, and sRANKL were determined by ELISA in 49 girls with AN and in 30 healthy controls, aged 13 to 17 years. Results: Girls with AN exhibited significant reduction in body weight, BMI, and Cole index as well as a significant increase in serum omentin-1 levels, compared to healthy participants. These changes were associated with a significant decrease in serum OC and CTx levels and a significant increase in OPG and sRANKL while the OC/CTx and OPG/sRANKL ratios were significantly decreased. BMI and the Cole index correlated negatively and significantly with omentin-1 levels, positively with CTx levels and the OC/CTx ratio in the control group (C), girls with AN, and all study participants (C + AN). Girls with AN showed a significant negative correlation between BMI, the Cole index, and OPG levels. The combined group (C + AN) showed a significant positive correlation between BMI, the Cole index, and the OPG/sRANKL ratio. Omentin-1 levels correlated negatively and significantly with OC and CTx levels as well as with the OC/CTx and OPG/sRANKL ratios in the C, AN, and C + AN groups. Conclusions:The relationship between omentin-1, bone markers, and the OC/CTx and OPG/sRANKL ratios observed in girls with AN indicates the involvement of this adipokine in the regulation of dynamic balance between bone formation and resorption processes. Omentin-1 might exert a negative effect on bone remodelling in girls with AN by inhibiting both bone formation and resorption. The OPG/sRANKL system plays an important role in the latter. (Endokrynol Pol 2015; 66 (6): 514-520)

TGF-β1, metabolizm kostny, osteoprotegeryna i rozpuszczalny ligand receptora aktywatora czynnika jądrowego-B u dziewcząt z jadłowstrętem psychicznym

“…This is in contrast to normal-weight adolescents, who have increased levels of bone metabolism markers, particularly in earlier puberty, consistent with increased bone modelling [15]. On the other hand, serum sRANKL [25][26][27][28][29][30][31] and/or OPG levels [23][24][25][26][27][28][29][30][31] are significantly increased in girls with AN while the OPG/sRANKL ratio is significantly decreased [32,[25][26][27][28][29][30][31]. MunozCalvo et al [32] were the only researchers who did not find significant changes in OPG levels in girls with AN who exhibited a significantly increased sRANKL level and OPG/sRANKL suppression.…”

“…Pomeroy et al [38] found significant elevation of TGF-β, while Corcos et al [39] found a significant decrease of TGF-β2, in young women with AN compared to the control participants. All these data [2,7,8,36], along with our previous results [25][26][27][28][29][30][31], indicate that the abnormal TGF-β concentrations in women with AN demonstrated by several authors [38,39] might promote the development of osteoporosis in these patients, possibly through OPG and/or RANKL. To the best of our knowledge, the relationship between potential changes in TGF-β1 concentrations and bone markers in girls with AN has not been studied so far.…”

“…IL-1, IL-6, TNF-α, INF-γ, and PGE 2 ) [15,[25][26][27][28][29][30][31][33][34][35][36], which modulate or are modulated by TGF-β1 signalling in a number of ways. The above-mentioned osteotropic factors might modify bone remodelling either directly (via specific receptors) or/and indirectly (via the RANKL/RANK/OPG system), and with or without TGF-β1 as a mediator [1, 15, 27-32, 34, 45-47].…”

“…Several studies have reported a positive relationship between body mass, body mass index (BMI), and/or bone mineral density (BMD) in adolescents with AN [15,[17][18][19][20][21][22]. Adolescents with AN also have lower levels of biochemical markers of bone formation and resorption than normal-weight controls, indicating a decrease in bone turnover [15,[23][24][25][26][27][28][29][30][31]. This is in contrast to normal-weight adolescents, who have increased levels of bone metabolism markers, particularly in earlier puberty, consistent with increased bone modelling [15].…”

“…MunozCalvo et al [32] were the only researchers who did not find significant changes in OPG levels in girls with AN who exhibited a significantly increased sRANKL level and OPG/sRANKL suppression. Our previous studies [26][27][28][29][30][31] indicate some desynchronisation between the RANKL/RANK/OPG system and bone remodelling (especially bone resorption) in girls with AN.…”