TGF-β1, metabolizm kostny, osteoprotegeryna i rozpuszczalny ligand receptora aktywatora czynnika jądrowego-B u dziewcząt z jadłowstrętem psychicznym

Abstract

Introduction: Numerous investigations, and especially in vitro studies, indicate that TGF-β1 may act as an important regulator of bone remodelling. Thus, it could be expected that disturbances of this cytokine production observed by several researchers might play a role in the mechanism leading to the development of osteoporosis in girls with anorexia nervosa (AN). The aim of the study was to determine whether 1) girls with AN exhibited a relationship between TGF-β1 and bone metabolism (as assessed based on serum OC and CTx concentrations) and 2) whether OPG and sRANKL might modify the possible relationship between TGF-β1 and bone metabolism. Material and methods: Serum concentrations of TGF-β, OC, CTx, OPG, and its soluble ligand sRANKL were determined by ELISA in 60 girls with AN and in 20 healthy controls (C). All study participants were aged 13 to 17 years. Results: Body weight, BMI, BMI-SDS and the Cole index, serum TGF-β1, OC, CTx, and the OPG/sRANKL ratio were significantly reduced, while OPG and sRANKL levels were significantly increased, in girls with AN compared to healthy participants. BMI and the Cole index correlated negatively and significantly with serum CTx and OPG (AN group) or CTx only (groups C and C + AN). Girls with AN showed a positive and significant correlation between the Cole index and serum TGF-β1. The combination group (C + AN) showed a positive and significant correlation between BMI, the Cole index, and the OPG/sRANKL ratio and TGF-β1 concentration, while TGF-β1 correlated positively and significantly with OC concentrations and the OPG/sRANKL ratio. The Cole index and BMI were identified to be significant and independent predictors of CTx (C, AN, and C+AN groups) and OPG (AN group); the Cole index, BMI, and TGF-β1 independently predicted the OPG/sRANKL ratio (C, AN, and C + AN groups); TGF-β1 was found to be an independent predictor of OC (C + AN group). Conclusions: Changes in bone markers, OPG, and/or OPG/sRANKL ratio observed in girls with AN are associated with changes in serum TGF-β1 concentrations. TGF-β1 suppression in girls with AN might lead to disturbances in the relationship between bone metabolism and the OPG/sRANKL system, which, in turn, might compromise the mechanism compensating for bone remodelling disturbances. (Endokrynol Pol 2016; 67 (5): 493-500)

Modestly degraded microarchitecture and high serum levels of osteopontin in Swedish females with anorexia nervosa

“…We could not demonstrate any correlations between TBS and bone turnover markers, but as anticipated we found decreased serum levels of the Wnt signalling pathway inhibitor, DKK-1 [25]. For OPG we measured increased serum levels, which confirms a finding by Ostrowska et al [26], although in patients with AN an increased secretion of RANKL, and decreased levels would be expected due to estrogen deficiency [27]. Osteopontin (OPN) is a non-collagenous matrix protein expressed in bone tissue, which is involved in bone remodeling and capable of activating bone resorption [28].…”

Effects of physical activity on sclerostin concentrations

“…Bone metabolic markers may be useful in the diagnosis and monitoring of not only osteoporosis [21,22], but may also be applied in other diseases that secondarily lead to bone metabolic disorders [23][24][25]. In our study we have assessed the effect of physical activity on changes in sclerostin and other known bone metabolism markers levels.…”

Eating disorders and bone metabolism in women

“…In AN, OPG and sRANKL levels are elevated, but the ratio of OPG to sRANKL is reduced. TGF- β secretion is reduced in AN, which may compromise the mechanism compensating for bone remodelling [17]. Omentin-1, secreted by visceral adipose tissue, is increased in AN, and has recently been suggested to exert a negative effect on bone remodelling [18], independent of menstrual status.…”